Comparison of the phytohaemagglutinin from red kidney bean (Phaseolus vulgaris) purified by different affinity chromatography

2008 ◽  
Vol 108 (1) ◽  
pp. 394-401 ◽  
Author(s):  
Jiaoyan Ren ◽  
John Shi ◽  
Yukio Kakuda ◽  
Daniel Kim ◽  
Sophia Jun Xue ◽  
...  
1979 ◽  
Vol 183 (2) ◽  
pp. 381-388 ◽  
Author(s):  
F Serafini-Cessi ◽  
C Franceschi ◽  
S Sperti

Human Tamm-Horsfall glycoprotein inhibits lymphocyte transformation induced by leucoagglutinin and haemagglutinin from Phaseolus vulgaris (red kidney bean). The glycoprotein interacts with the two lectins, giving insoluble precipitates. The interaction with leucoagglutinin is highly specific, and the shape of the precipitin curve is that of an antigen-antibody reaction; precipitation is specifically inhibited by N-acetyl-D-galactosamine. Results are discussed, and it is suggested that inhibition of lymphocyte transformation is due to competition between human Tamm-Horsfall glycoprotein and carbohydrate receptors on lymphocytes for the two lectins. The interaction between human Tamm-Horsfall glycoprotein and Phaseolus vulgaris lectins has been used to develop a one-step procedure for the separation of the two lectins by affinity chromatography on (human Tamm-Horsfall-glycoprotein)-Sepharose.


Neonatology ◽  
2003 ◽  
Vol 84 (2) ◽  
pp. 152-158 ◽  
Author(s):  
Danuta Kruszewska ◽  
Pawel Kiela ◽  
Åsa Ljungh ◽  
Kennedy H. Erlwanger ◽  
Björn R. Weström ◽  
...  

1995 ◽  
Vol 108 (4) ◽  
pp. A730 ◽  
Author(s):  
K.H. Herzig ◽  
S. Bardocz ◽  
G. Grant ◽  
R. Nustede ◽  
U.R. Fölsch ◽  
...  

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