scholarly journals The impact of a baked muffin matrix on the bioaccessibility and IgE reactivity of egg and peanut allergens

2021 ◽  
pp. 129879
Author(s):  
Hadeer Mattar ◽  
Phil Padfield ◽  
Angela Simpson ◽  
E.N. Clare Mills
Keyword(s):  
Ige Reactivity ◽  
Peanut Allergens ◽  
The Impact

scholarly journals The Effect of the Food Matrix on the In Vitro Bio‐Accessibility and IgE Reactivity of Peanut Allergens

2020 ◽  
Vol 64 (14) ◽  
pp. 1901093
Author(s):  
Huan Rao ◽  
Ivona Baricevic ◽  
Hervé Bernard ◽  
Frances Smith ◽  
Rebekah Sayers ◽  
...  
Keyword(s):  
Food Matrix ◽  
Ige Reactivity ◽  
Peanut Allergens

scholarly journals Hazelnut (Corylus avellana) vicilin Cor a 11: molecular characterization of a glycoprotein and its allergenic activity

2004 ◽  
Vol 383 (2) ◽  
pp. 327-334 ◽  
Author(s):  
Iris LAUER ◽  
Kay FOETISCH ◽  
Daniel KOLARICH ◽  
Barbara K. BALLMER-WEBER ◽  
Amedeo CONTI ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Corylus Avellana ◽  
Cd Spectroscopy ◽  
Mediator Release ◽  
Food Allergies ◽  
Ige Reactivity ◽  
Allergenic Activity ◽  
The Impact

In Europe, hazelnuts (Corylus avellana) are a frequent cause of food allergies. Several important hazelnut allergens have been previously identified and characterized. Specific N-glycans are known to induce strong IgE responses of uncertain clinical relevance, but so far the allergenic potential of glycoproteins from hazelnut has not been investigated. The aim of the study was the molecular characterization of the glycosylated vicilin Cor a 11 from hazelnut and the analysis of its allergenic activity. Although MALDI–TOF (matrix-assisted laser-desorption ionization–time-of-flight) MS showed that one of two potential glycosylation sites of Cor a 11 was glycosylated, CD spectroscopy indicated that recombinant and natural Cor a 11 share similar secondary structures. Thus to analyse the impact of the glycan residues of Cor a 11 on IgE binding, the allergenic activity of natural glycosylated Cor a 11 and recombinant Cor a 11 was compared. In addition, the IgE sensitization pattern to recombinant Cor a 11, Cor a 1, Cor a 2 and Cor a 8 of 65 hazelnut allergic patients was determined in vitro. The prevalence of IgE reactivity to hazelnut vicilin Cor a 11 was below 50%. Basophil histamine-release assays were used to determine the allergenic activity of both natural and recombinant Cor a 11 in comparison with Cor a 1, a birch (Betula verrucosa) pollen-related major hazelnut allergen. Both forms of Cor a 11 induced mediator release from basophils to a similar extent, indicating that the hazelnut allergic patients had cross-linking IgE antibodies binding to the protein backbone and not to carbohydrate structures. In comparison to Cor a 1, a 10000-fold higher concentration of Cor a 11 was required to induce similar basophil mediator release. In conclusion, the hazelnut vicilin Cor a 11 is a minor allergen both in regard to prevalence and allergenic potency, whereas its glycan does not contribute to its allergenic activity.

Work-Related Allergy and Asthma in Spice Mill Workers – The Impact of Processing Dried Spices on IgE Reactivity Patterns

2010 ◽  
Vol 152 (3) ◽  
pp. 271-278 ◽  
Author(s):  
Anita van der Walt ◽  
Andreas L. Lopata ◽  
Natalie E. Nieuwenhuizen ◽  
Mohamed F. Jeebhay
Keyword(s):  
Work Related ◽  
Ige Reactivity ◽  
The Impact ◽  
Allergy And Asthma

Roasting and lipid binding provide allergenic and proteolytic stability to the peanut allergen Ara h 8

2014 ◽  
Vol 395 (2) ◽  
pp. 239-250 ◽  
Author(s):  
Arnd Petersen ◽  
Sandra Rennert ◽  
Skadi Kull ◽  
Wolf-Meinhard Becker ◽  
Holger Notbohm ◽  
...  
Keyword(s):  
Birch Pollen ◽  
Lipid Binding ◽  
Homologous Proteins ◽  
Peanut Allergen ◽  
Maillard Reactions ◽  
Bet V 1 ◽  
Ige Reactivity ◽  
Hydrophobic Binding ◽  
Proteolytic Stability ◽  
The Impact

Abstract Ara h 8 is the peanut allergen homologous to the birch pollen allergen Bet v 1. Because Bet v 1 has been shown to bind lipophilic ligands, the aim of this investigation was to determine the impact of lipid binding and roasting on the Ara h 8 structure and their influences on allergenicity. For the characterization of natural Ara h 8 (nAra h 8) from roasted and unroasted peanuts, circular dichroism spectroscopy, hydrophobic binding assay, immunohistochemistry, and immunoblot with sera of peanut allergic patients were performed and compared with results from recombinant Ara h 8 (rAra h 8) and Bet v 1. rAra h 8 displayed stronger hydrophobicity than rBet v 1. Patients’ sera showed IgE reactivity with rAra h 8 and nAra h 8 from roasted peanuts, whereas fewer sera recognized nAra h 8 from unroasted peanuts. Simulated gastric digestion experiments demonstrated low proteolytic stability of rAra h 8, whereas the stability of nAra h 8 was increasingly higher in unroasted and roasted peanuts. The results demonstrate that IgE reactivity and thermal and proteolytic stability are reinforced in nAra h 8 after roasting, most likely due to Maillard reactions, lipid oxidations, and lipophilic associations. These aspects must be considered when estimating the allergenicity of Bet v 1-homologous proteins.

scholarly journals What makes peanuts so allergenic?

2013 ◽  
Vol 78 (3) ◽  
pp. 321-331 ◽  
Author(s):  
Arnd Petersen ◽  
Wolf Becker ◽  
Uta Jappe
Keyword(s):  
Storage Proteins ◽  
Food Allergies ◽  
Heat Denaturation ◽  
Allergic Reactions ◽  
Food Allergens ◽  
Geographical Differences ◽  
Ige Reactivity ◽  
Ara H 1 ◽  
Peanut Allergens ◽  
Inhalant Allergens

Peanut allergy belongs to one of the most severe food allergies. So far 12 peanut allergens have been registered by the IUIS Allergen Nomenclature Subcommittee. Here, we describe the different peanut allergens and factors that contribute to allergenicity. Peanut contains several class I food allergens (especially Ara h 1, 2, 3) that are stable against heat denaturation and proteolytic digestion and represent storage proteins. These allergens are often associated with severe allergic reactions. Additionally, peanut contains class II food allergens (Ara h 5 and 8), where the IgE reactivity is caused by cross reactions to inhalant allergens. These allergens are mostly associated with mild to moderate allergic reactions. But the severity of symptoms may change by involvement of additional factors. The peanut matrix consists of about 50% of lipids, and allergen - lipid associations have been shown for several peanut allergens. Further factors influencing allergenicity depend on peanut varieties, geographical differences and alterations in food processing. Finally, the physiological function of allergens and the mechanisms, by which they interact with the immune system, are further modulating factors. Thus, the specific allergen structure, matrix, genetic variations, geographic alterations and further augmentation factors are important parameters that induce and influence allergenicity.

Formation of Lunar Craters and Bright Rays as a Result of Meteorite Impacts

1962 ◽  
Vol 14 ◽  
pp. 415-418
Author(s):  
K. P. Stanyukovich ◽  
V. A. Bronshten
Keyword(s):  
Explosive Charge ◽  
The Moon ◽  
Meteorite Impacts ◽  
The Earth ◽  
Lunar Craters ◽  
The Impact

The phenomena accompanying the impact of large meteorites on the surface of the Moon or of the Earth can be examined on the basis of the theory of explosive phenomena if we assume that, instead of an exploding meteorite moving inside the rock, we have an explosive charge (equivalent in energy), situated at a certain distance under the surface.

The Geosciences Applied to Lunar Exploration

1962 ◽  
Vol 14 ◽  
pp. 169-257 ◽  
Author(s):  
J. Green
Keyword(s):  
Lunar Surface ◽  
Probable Mechanism ◽  
Point Of View ◽  
Lunar Exploration ◽  
Geological Model ◽  
Apply Geophysics ◽  
Surface Features ◽  
The Impact

The term geo-sciences has been used here to include the disciplines geology, geophysics and geochemistry. However, in order to apply geophysics and geochemistry effectively one must begin with a geological model. Therefore, the science of geology should be used as the basis for lunar exploration. From an astronomical point of view, a lunar terrain heavily impacted with meteors appears the more reasonable; although from a geological standpoint, volcanism seems the more probable mechanism. A surface liberally marked with volcanic features has been advocated by such geologists as Bülow, Dana, Suess, von Wolff, Shaler, Spurr, and Kuno. In this paper, both the impact and volcanic hypotheses are considered in the application of the geo-sciences to manned lunar exploration. However, more emphasis is placed on the volcanic, or more correctly the defluidization, hypothesis to account for lunar surface features.

Asteroid and Comet Impact Speeds upon Mars: Significance for Panspermia and the Supply of Organics

1997 ◽  
Vol 161 ◽  
pp. 197-201 ◽  
Author(s):  
Duncan Steel
Keyword(s):  
Probability Distributions ◽  
Regions Of Interest ◽  
Significant Fraction ◽  
Organic Chemicals ◽  
Impact Speed ◽  
The Mean ◽  
The Impact

AbstractWhilst lithopanspermia depends upon massive impacts occurring at a speed above some limit, the intact delivery of organic chemicals or other volatiles to a planet requires the impact speed to be below some other limit such that a significant fraction of that material escapes destruction. Thus the two opposite ends of the impact speed distributions are the regions of interest in the bioastronomical context, whereas much modelling work on impacts delivers, or makes use of, only the mean speed. Here the probability distributions of impact speeds upon Mars are calculated for (i) the orbital distribution of known asteroids; and (ii) the expected distribution of near-parabolic cometary orbits. It is found that cometary impacts are far more likely to eject rocks from Mars (over 99 percent of the cometary impacts are at speeds above 20 km/sec, but at most 5 percent of the asteroidal impacts); paradoxically, the objects impacting at speeds low enough to make organic/volatile survival possible (the asteroids) are those which are depleted in such species.

Sign in / Sign up

Export Citation Format

Share Document