scholarly journals Isolation of recombinant antibody fragments (scFv) by phage display technology for detection of almond allergens in food products

Food Control ◽  
2015 ◽  
Vol 54 ◽  
pp. 322-330 ◽  
Author(s):  
Silvia de la Cruz ◽  
Carolina Cubillos-Zapata ◽  
Inés María López-Calleja ◽  
Satyabrata Ghosh ◽  
Marcos Alcocer ◽  
...  
Keyword(s):  
Phage Display ◽  
Recombinant Antibody ◽  
Food Products ◽  
Antibody Fragments ◽  
Phage Display Technology ◽  
Display Technology ◽  
Recombinant Antibody Fragments

Characterization of 11-dehydro-thromboxane B2 recombinant antibody obtained by phage display technology

2003 ◽  
Vol 68 (4) ◽  
pp. 273-284 ◽  
Author(s):  
Lilian Rumi Tsuruta ◽  
Yoshihisa Tomioka ◽  
Takanori Hishinuma ◽  
Yoshinori Kato ◽  
Kunihiko Itoh ◽  
...  
Keyword(s):  
Phage Display ◽  
Recombinant Antibody ◽  
Thromboxane B2 ◽  
Phage Display Technology ◽  
Display Technology

scholarly journals Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7652
Author(s):  
Wei Sun ◽  
Yan Zhang ◽  
Zhigang Ju
Keyword(s):  
Phage Display ◽  
Recombinant Antibody ◽  
Recombinant Antibodies ◽  
Phage Display Library ◽  
Ease Of Use ◽  
Phage Library ◽  
Phage Display Technology ◽  
Random Peptide ◽  
Display Technology

Mycotoxins, the small size secondary metabolites of fungi, have posed a threat to the safety of medicine, food and public health. Therefore, it is essential to create sensitive and effective determination of mycotoxins. Based on the special affinity between antibody and antigen, immunoassay has been proved to be a powerful technology for the detection of small analytes. However, the tedious preparation and instability of conventional antibodies restrict its application on easy and fast mycotoxins detection. By virtue of simplicity, ease of use, and lower cost, phage display library provides novel choices for antibodies or hapten conjugates, and lead random peptide or recombinant antibody to becoming the promising and environmental friendly immune-reagents in the next generation of immunoassays. This review briefly describes the latest developments on mycotoxins detection using M13 phage display, mainly focusing on the recent applications of phage display technology employed in mycotoxins detection, including the introduction of phage and phage display, the types of phage displayed peptide/recombinant antibody library, random peptides/recombinant antibodies-based immunoassays, as well as simultaneous determination of multiple mycotoxins.

scholarly journals Improved Soluble ScFv ELISA Screening Approach for Antibody Discovery Using Phage Display Technology

2017 ◽  
Vol 22 (8) ◽  
pp. 1026-1034 ◽  
Author(s):  
Mohammad R. Tohidkia ◽  
Maryam Sepehri ◽  
Shirin Khajeh ◽  
Jaleh Barar ◽  
Yadollah Omidi
Keyword(s):  
Phage Display ◽  
Recombinant Antibody ◽  
False Negative ◽  
Protein A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Phage Display Technology ◽  
Negative Results ◽  
Specific Phage ◽  
Display Technology ◽  
The Individual

Phage display technology (PDT) is a powerful tool for the isolation of recombinant antibody (Ab) fragments. Using PDT, target molecule-specific phage-Ab clones are enriched through the “biopanning” process. The individual specific binders are screened by the monoclonal scFv enzyme-linked immunosorbent assay (ELISA) that may associate with inevitable false-negative results. Thus, in this study, three strategies were investigated for optimization of the scFvs screening using Tomlinson I and J libraries, including (1) optimizing the expression of functional scFvs, (2) improving the sensitivity of ELISA, and (3) preparing different samples containing scFvs. The expression of all scFv Abs was significantly enhanced when scFv clones were cultivated in the terrific broth (TB) medium at the optimum temperature of 30 °C. The protein A–conjugated with horseradish peroxidase (HRP) was found to be a well-suited reagent for the detection of Ag-bound scFvs in comparison with either anti-c-myc Ab or the mixing procedure. Based on our findings, it seems there is no universal media supplement for an improved expression of all scFvs derived from both Tomlinson I and J libraries. We thus propose that expression of scFv fragments in a microplate scale is largely dependent on a variety of parameters, in particular the scFv clones and relevant sequences.

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