New insights into the NaCl impact on emulsifying properties of globular proteins

2022 ◽  
Vol 124 ◽  
pp. 107342
Author(s):  
Lili Zhang ◽  
Wei-Feng Lin ◽  
Yin Zhang ◽  
Chuan-He Tang
Author(s):  
J. L. Farrant ◽  
J. D. McLean

For electron microscope techniques such as ferritin-labeled antibody staining it would be advantageous to have available a simple means of thin sectioning biological material without subjecting it to lipid solvents, impregnation with plastic monomers and their subsequent polymerization. With this aim in view we have re-examined the use of protein as an embedding medium. Gelatin which has been used in the past is not very satisfactory both because of its fibrous nature and the high temperature necessary to keep its solutions fluid. We have found that globular proteins such as the serum and egg albumins can be cross-linked so as to yield blocks which are suitable for ultrathin sectioning.


2014 ◽  
Vol 11 (1) ◽  
pp. 29
Author(s):  
Nonnah Ismail ◽  
Juliana Mahmod ◽  
Awatif Khairul Fatihin Mustafa Kamal

In this study, Hydrolysate from angelwing clam (Pholas orientalis) was produced at 0, 1, 2 and 3 hrs and E/S ratio of0.5 and 3%using alcalase where the pH and temperature were kept constant at pH 8.5 and 60°C, respectively. The hydrolysates were analysed for antioxidant and functional properties such as solubility, emulsifying properties and water and oil holding capacity. Degree of hydrolysis (DH), yield, functional and antioxidant properties were influenced by the hydrolysis time and E/S ratio. Higher enzyme concentration (E/S 3%) and longer hydrolysis time increased the DH. Yield was higher at E/S 3% but reduced with hydrolysis time. Longer hydrolysis time produced more soluble hydrolysate and higher metal chelating activity but lower in emulsifying properties and DPPH activity. Higher enzyme concentration resulted in increase only in solubility and metal chelating activity. This study revealed that enzymatic hydrolysis using alcalase should be performed at shorter hydrolysis time using intermediate concentration of enzyme (E/S between 0.5 to 3%) in order to produce angelwing clam hydrolysate with collectively good functional and antioxidant properties. 


2013 ◽  
Vol 30 (1) ◽  
pp. 73
Author(s):  
Wei JING ◽  
Jun-sheng LI ◽  
Guo-xia HUANG ◽  
Liu-juan YAN ◽  
Chun-hua LAI

2014 ◽  
Vol 14 (5) ◽  
pp. 630-639 ◽  
Author(s):  
Najet Mahmoudi ◽  
Cedric Gaillard ◽  
Alain Riaublanc ◽  
Francois Boue ◽  
Monique Axelos

2021 ◽  
Vol 113 ◽  
pp. 106516
Author(s):  
Yongjian Cai ◽  
Lihua Huang ◽  
Bifen Chen ◽  
Jiaqi Su ◽  
Xiujie Zhao ◽  
...  

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