Free radical scavenging action of the natural polyamine spermine in rat liver mitochondria

The relationship between the quantitative ratio of redox forms of ubiquinone and the degree of free radical damage to mitochondrial proteins in rat liver against the background of nutritional imbalance was investigated. The animals were divided into the following experimental groups: I – animals receiving full-value semi-synthetic ration (control group); II – animals receiving high-sucrose diet; III – animals receiving low-protein high-sucrose diet. The content of total and oxidized ubiquinone was determined spectrophotometrically at 275 nm, the content of reduced ubiquinone was determined by the difference between the content of total and oxidized ubiquinone. The intensity of the oxidative modification of proteins was assessed by the accumulation of carbonyl derivatives in the reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH), the content of free SH-groups was assessed by using the Elman reagent. It was found that the most pronounced decrease in the content of total ubiquinone (almost twice) and the redistribution of its redox forms (reduction of the content of reduced ubiquinone by 7.2 times against the background of an increase in the level of oxidized ubiquinone by 2 times) in rat liver mitochondria is observed in animals that received a diet high in sucrose against the background of alimentary protein deprivation. In addition, the animals of this group showed the most pronounced free radical oxidation of mitochondrial proteins, as evidenced by a 3.5-fold increase in the content of carbonyl derivatives and a 2.6-fold decrease in the content of free protein SH- groups. It was shown that nutritional protein deficiency is a critical factor affecting the intensity of free radical processes in mitochondria. The established changes in the ratio of the redox forms of ubiquinone and the degree of oxidative modification of mitochondrial proteins in rat liver could be considered as prerequisites for deepening the energy imbalance and violation of the functional activity of mitochondria under conditions of nutritional imbalance.

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Antioxidant and Free Radical-Scavenging Properties of Stevia rebaudiana (Bertoni) Extracts and L-NNA in Streptozotocine-Nicotinamide Induced Diabetic Rat Liver

Turkiye Klinikleri Journal of Medical Sciences ◽

10.5336/medsci.2009-16216 ◽

2011 ◽

Vol 31 (1) ◽

pp. 51-60 ◽

Cited By ~ 2

Author(s):

Cansu ÖZBAYER ◽

İrfan DEĞİRMENCİ ◽

Hülyam KURT ◽

Hilmi ÖZDEN ◽

Kısmet ÇİVİ ◽

...

Keyword(s):

Free Radical ◽

Rat Liver ◽

Radical Scavenging ◽

Stevia Rebaudiana ◽

Free Radical Scavenging ◽

Diabetic Rat ◽

Stevia Rebaudiana Bertoni ◽

Radical Scavenging Properties

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Effects of iron loading on free radical scavenging enzymes and lipid peroxidation in rat liver

Gastroenterology ◽

10.1016/0016-5085(89)91511-4 ◽

1989 ◽

Vol 97 (4) ◽

pp. 1011-1018 ◽

Cited By ~ 37

Author(s):

Linda M. Fletcher ◽

Faye D. Roberts ◽

Michael G. Irving ◽

Lawrie W. Powell ◽

June W. Halliday

Keyword(s):

Lipid Peroxidation ◽

Free Radical ◽

Rat Liver ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Iron Loading ◽

Scavenging Enzymes

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Free-radical metabolism of carbon tetrachloride in rat liver mitochondria. A study of the mechanism of activation

Biochemical Journal ◽

10.1042/bj2460313 ◽

1987 ◽

Vol 246 (2) ◽

pp. 313-317 ◽

Cited By ~ 60

Author(s):

A Tomasi ◽

E Albano ◽

S Banni ◽

B Botti ◽

F Corongiu ◽

...

Keyword(s):

Free Radical ◽

Carbon Tetrachloride ◽

Rat Liver ◽

Liver Microsomes ◽

Liver Mitochondria ◽

Rat Liver Mitochondria ◽

Radical Species ◽

Hypoxic Conditions ◽

Free Radical Species ◽

Transport Chain

Alterations in liver mitochondria as consequence of rat poisoning with carbon tetrachloride (CCl4) have been reported over many years, but the mechanisms responsible for causing such damage are still largely unknown. Isolated rat liver mitochondria incubated under hypoxic conditions with succinate and ADP were found able to activate CCl4 to a free-radical species identified as trichloromethyl free radical (CCl3) by e.s.r. spectroscopy coupled with the spin-trapping technique. The incubation of mitochondria in air decreased free-radical production, indicating that a reductive reaction was involved in the activation of CCl4. However, in contrast with liver microsomes (microsomal fractions), mitochondria did not require the presence of NADPH, and the process was not significantly influenced by inhibitors of cytochrome P-450. The addition of inhibitors of the respiratory chain such as antimycin A and KCN decreased free-radical formation by only 30%, whereas rotenone displayed a greater effect (approx. 84% inhibition), but only when preincubated for 15 min with mitochondria not supplemented with succinate. These findings suggest that the mitochondrial electron-transport chain is responsible for the activation of CCl4. A conjugated-diene band was observed in the lipids extracted from mitochondria incubated with CCl4 under anaerobic conditions, indicating that stimulation of lipid peroxidation was occurring as a result of the formation of free-radical species.

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Antioxidant and Antiplatelet Effects of Dang-Gui-Shao-Yao-San on Human Blood Cells

The American Journal of Chinese Medicine ◽

10.1142/s0192415x05003351 ◽

2005 ◽

Vol 33 (05) ◽

pp. 747-758 ◽

Cited By ~ 21

Author(s):

Ai-Yu Shen ◽

Trey-Shy Wang ◽

Mei-Han Huang ◽

Chang-Hui Liao ◽

Sheue-Jiun Chen ◽

...

Keyword(s):

Lipid Peroxidation ◽

Free Radical ◽

Rat Liver ◽

Superoxide Anion ◽

Radical Scavenging ◽

Liver Homogenate ◽

Free Radical Scavenging ◽

Human Neutrophils ◽

Dependent Manner ◽

Superoxide Formation

Dang-Gui-Shao-Yao-San (DGSYS) is a mixture of medicinal herbs, which has long been used in traditional Chinese medicine for treating anemia and ovulary disorders. Its preparation comprises Angelicae sinensis (Oliv.) Diels, Ligustucum chuanxiong Hort, Paeonia lactiflora pall, Poria cocos (Schw.) Wolf, Atractylodis macrocephala Koidz and Alisma orientalis (Sam.) Juzep. The present study examined the anti-superoxide formation, free radical scavenging and anti-lipid peroxidation activities of DGSYS by xanthine oxidase inhibition, cytochrome C system with superoxide anion released by the fMLP or PMA activating pathway in human neutrophils, and FeCl 2 ascorbic acid-induced lipid peroxidation effects on lipids in rat liver homogenate, respectively. DGSYS showed anti-superoxide formation and free radical scavenging activity in a concentration-dependent manner. It also inhibited PMA- but not fMLP-induced superoxide anion released from human neutrophils. These antioxidant actions of DGSYS showed beneficial cytoprotective effects against lipid peroxidation in rat liver homogenate, human platelet aggregation induced by arachidonic acid (AA) and adenosine diphosphate (ADP) and mitomycin C-mediated hemolytic in human erythrocytes.

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