Conjugational transfer system to shuttle giant DNA cloned by Bacillus subtilis genome (BGM) vector

Gene ◽  
2007 ◽  
Vol 399 (1) ◽  
pp. 72-80 ◽  
Author(s):  
Azusa Kuroki ◽  
Naoto Ohtani ◽  
Kenji Tsuge ◽  
Masaru Tomita ◽  
Mitsuhiro Itaya
Keyword(s):  
Bacillus Subtilis ◽  
Transfer System ◽  
Conjugational Transfer ◽  
Subtilis Genome

scholarly journals Targeted Isolation of a Designated Region of the Bacillus subtilis Genome by Recombinational Transfer

2004 ◽  
Vol 70 (4) ◽  
pp. 2508-2513 ◽  
Author(s):  
Satoshi Tomita ◽  
Kenji Tsuge ◽  
Yo Kikuchi ◽  
Mitsuhiro Itaya
Keyword(s):  
Bacillus Subtilis ◽  
Copy Number ◽  
Positional Cloning ◽  
Deletion Mutant ◽  
Essential Gene ◽  
Transfer System ◽  
Copy Number Plasmid ◽  
Low Copy Number ◽  
Subtilis Genome

ABSTRACT A method for positional cloning of the Bacillus subtilis genome was developed. The method requires a set of two small DNA fragments that flank the region to be copied. A 38-kb segment that carries genes ppsABCDE encoding five enzymes for antibiotic plipastatin synthesis and another genome locus as large as 100 kb including one essential gene were examined for positional cloning. The positional cloning vector for ppsABCDE was constructed using a B. subtilis low-copy-number plasmid that faithfully copied the precise length of the 38-kb DNA in vivo via the recombinational transfer system of this bacterium. Structure of the copied DNA was confirmed by restriction enzyme analyses. Furthermore, the unaltered structure of the 38-kb DNA was demonstrated by complementation of a ppsABCDE deletion mutant.

scholarly journals Far rapid synthesis of giant DNA in the Bacillus subtilis genome by a conjugation transfer system

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Mitsuhiro Itaya ◽  
Mitsuru Sato ◽  
Miki Hasegawa ◽  
Nobuaki Kono ◽  
Masaru Tomita ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Transfer System ◽  
Rapid Synthesis ◽  
Conjugation Transfer ◽  
Subtilis Genome

scholarly journals Subtilist: a relational database for the Bacillus subtilis genome

Microbiology ◽  
1995 ◽  
Vol 141 (2) ◽  
pp. 261-268 ◽  
Author(s):  
I. Moszer ◽  
P. Glaser ◽  
A. Danchin
Keyword(s):  
Bacillus Subtilis ◽  
Relational Database ◽  
Subtilis Genome

scholarly journals The systematic sequencing of the Bacillus subtilis genome in Japan

Microbiology ◽  
1996 ◽  
Vol 142 (11) ◽  
pp. 2993-2994 ◽  
Author(s):  
N. Ogasawara ◽  
H. Yoshikawa
Keyword(s):  
Bacillus Subtilis ◽  
Subtilis Genome

scholarly journals Application of recombination transfer to the cognate Bacillus subtilis genome

2003 ◽  
Vol 3 (1) ◽  
pp. 295-296
Author(s):  
S. Tomita ◽  
K. Tsuge ◽  
Y. Kikuchi ◽  
M. Itaya
Keyword(s):  
Bacillus Subtilis ◽  
Subtilis Genome
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