Vascular endothelial growth factor-D is overexpressed in human cardiac allograft vasculopathy and diabetic atherosclerosis and induces endothelial permeability to low-density lipoproteins in vitro

2011 ◽  
Author(s):  
Brian W. Wong ◽  
Donald Wong ◽  
Honglin Luo ◽  
Bruce M. McManus
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Endothelial Permeability ◽  
Cardiac Allograft Vasculopathy ◽  
Low Density Lipoproteins ◽  
Cardiac Allograft ◽  
Low Density ◽  
Vascular Endothelial ◽  
Allograft Vasculopathy ◽  
Endothelial Growth Factor

In vitro release of vascular endothelial growth factor during platelet aggregation

1998 ◽  
Vol 275 (3) ◽  
pp. H1054-H1061 ◽  
Author(s):  
James P. Maloney ◽  
Christopher C. Silliman ◽  
Daniel R. Ambruso ◽  
Jun Wang ◽  
Rubin M. Tuder ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Platelet Aggregation ◽  
Growth Factor ◽  
Platelet Rich Plasma ◽  
In Vitro Release ◽  
Endothelial Permeability ◽  
Vascular Endothelial ◽  
Vessel Injury ◽  
Endothelial Growth Factor

Platelet aggregation is a cardinal feature of both vascular repair and vascular disease. During aggregation platelets release a variety of vasoactive substances; some of these promote angiogenesis, endothelial permeability, and endothelial growth, actions shared by vascular endothelial growth factor (VEGF). This study was undertaken to investigate the hypothesis that VEGF is released by aggregating platelets. We found that VEGF was secreted during the in vitro aggregation of platelet-rich plasma induced by thrombin, collagen, epinephrine, and ADP (range 23–518 pg VEGF/ml). Furthermore, serum VEGF levels were elevated compared with plasma (230 ± 63 vs. 38 ± 8 pg VEGF/ml), indicative of VEGF release during whole blood coagulation. Lysates of apheresed, leukocyte-poor platelet units contained significant amounts of VEGF (2.4 ± 0.8 pg VEGF/mg protein). VEGF message and protein were also present in a megakaryocytic cell line (Dami cell). These results suggest constitutive roles for platelet VEGF in the repair of intimal vessel injury and in the altered permeability and intimal proliferation seen at sites of platelet aggregation and thrombosis.

scholarly journals Streptococcus pneumoniae Induces Secretion of Vascular Endothelial Growth Factor by Human Neutrophils

2000 ◽  
Vol 68 (8) ◽  
pp. 4792-4794 ◽  
Author(s):  
Michiel van der Flier ◽  
Frank Coenjaerts ◽  
Jan L. L. Kimpen ◽  
Andy M. Hoepelman ◽  
Sibyl P. M. Geelen
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Vascular Permeability ◽  
Pneumococcal Disease ◽  
Endothelial Permeability ◽  
Human Neutrophils ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor ◽  
Stimulation Of

ABSTRACT Infection by pneumococci causes an acute inflammatory response associated with neutrophil influx, increased vascular permeability, and edema. Vascular endothelial growth factor (VEGF) is one of the most potent regulators of endothelial permeability. In vitro stimulation of neutrophils showed that pneumococci and purified pneumococcal cell wall induce VEGF secretion, independent of the presence of pneumolysin or polysaccharide capsule. The results of this study indicate VEGF is secreted in pneumococcal disease, suggesting a role as a mediator of increased vascular permeability.

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