scholarly journals In vitro and in silico characterization of alkaline serine protease from Bacillus subtilis D9 recovered from Saudi Arabia

Heliyon ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. e08148
Author(s):  
Amal Mahmoud ◽  
Essam Kotb ◽  
Amany I. Alqosaibi ◽  
Ahmed A. Al-Karmalawy ◽  
Ibtesam S. Al-Dhuayan ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Bacillus Subtilis ◽  
Serine Protease ◽  
In Silico ◽  
Alkaline Serine Protease ◽  
In Silico Characterization

IN VITRO/IN SILICO CHARACTERIZATION OF ACTIVE AND PASSIVE STRESSES IN CARDIAC MUSCLE

2012 ◽  
Vol 10 (2) ◽  
pp. 171-188 ◽  
Author(s):  
Markus Boel ◽  
Oscar J. Abilez ◽  
Ahmed N Assar ◽  
Christopher K. Zarins ◽  
Ellen Kuhl
Keyword(s):  
Cardiac Muscle ◽  
In Silico ◽  
In Silico Characterization

In-Vitro and In-Silico Characterization of Xylose Reductase from Emericella nidulans

2019 ◽  
Vol 13 (2) ◽  
pp. 159-170 ◽  
Author(s):  
Vishal Ahuja ◽  
Aashima Sharma ◽  
Ranju Kumari Rathour ◽  
Vaishali Sharma ◽  
Nidhi Rana ◽  
...  
Keyword(s):  
Production Process ◽  
In Silico ◽  
Xylose Reductase ◽  
In Silico Analysis ◽  
Emericella Nidulans ◽  
Higher Temperature ◽  
In Silico Characterization ◽  
Silico Analysis

Background: Lignocellulosic residues generated by various anthropogenic activities can be a potential raw material for many commercial products such as biofuels, organic acids and nutraceuticals including xylitol. Xylitol is a low-calorie nutritive sweetener for diabetic patients. Microbial production of xylitol can be helpful in overcoming the drawbacks of traditional chemical production process and lowring cost of production. Objective: Designing efficient production process needs the characterization of required enzyme/s. Hence current work was focused on in-vitro and in-silico characterization of xylose reductase from Emericella nidulans. Methods: Xylose reductase from one of the hyper-producer isolates, Emericella nidulans Xlt-11 was used for in-vitro characterization. For in-silico characterization, XR sequence (Accession No: Q5BGA7) was used. Results: Xylose reductase from various microorganisms has been studied but the quest for better enzymes, their stability at higher temperature and pH still continues. Xylose reductase from Emericella nidulans Xlt-11 was found NADH dependent and utilizes xylose as its sole substrate for xylitol production. In comparison to whole cells, enzyme exhibited higher enzyme activity at lower cofactor concentration and could tolerate higher substrate concentration. Thermal deactivation profile showed that whole cell catalysts were more stable than enzyme at higher temperature. In-silico analysis of XR sequence from Emericella nidulans (Accession No: Q5BGA7) suggested that the structure was dominated by random coiling. Enzyme sequences have conserved active site with net negative charge and PI value in acidic pH range. Conclusion: Current investigation supported the enzyme’s specific application i.e. bioconversion of xylose to xylitol due to its higher selectivity. In-silico analysis may provide significant structural and physiological information for modifications and improved stability.

scholarly journals In vitro and in silico characterization of metagenomic soil-derived cellulases capable of hydrolyzing oil palm empty fruit bunch

2017 ◽  
Vol 15 ◽  
pp. 55-62 ◽  
Author(s):  
Laura Marcela Palma Medina ◽  
Diana Catalina Ardila ◽  
María Mercedes Zambrano ◽  
Silvia Restrepo ◽  
Andrés Fernando González Barrios
Keyword(s):  
Oil Palm ◽  
In Silico ◽  
Empty Fruit Bunch ◽  
In Silico Characterization

scholarly journals In vitro and in silico characterization of a novel dextranase from Pochonia chlamydosporia

3 Biotech ◽  
2018 ◽  
Vol 8 (3) ◽  
Author(s):  
Bruna Leite Sufiate ◽  
Filippe Elias de Freitas Soares ◽  
Samara Silveira Moreira ◽  
Angélica de Souza Gouveia ◽  
Evandro Ferreira Cardoso ◽  
...  
Keyword(s):  
In Silico ◽  
Pochonia Chlamydosporia ◽  
In Silico Characterization

scholarly journals In Vitro and In Silico Characterization of Antimalarial Myristicyl Propanoate

2020 ◽  
Vol 499 ◽  
pp. 012010
Author(s):  
Kamilia Mustikasari ◽  
Joshua Eka Harap ◽  
Noor Cahaya
Keyword(s):  
In Silico ◽  
In Silico Characterization

In Vitro and in Silico Characterization of Peroxiredoxin 6 Modified by 4-Hydroxynonenal and 4-Oxononenal

2008 ◽  
Vol 21 (12) ◽  
pp. 2289-2299 ◽  
Author(s):  
James R. Roede ◽  
David L. Carbone ◽  
Jonathan A. Doorn ◽  
Oleg V. Kirichenko ◽  
Philip Reigan ◽  
...  
Keyword(s):  
In Silico ◽  
Peroxiredoxin 6 ◽  
In Silico Characterization

In vitro and in silico characterization of fibrous scaffolds comprising alternate colistin sulfate-loaded and heat-treated polyvinyl alcohol nanofibrous sheets

2017 ◽  
Vol 523 (1) ◽  
pp. 151-158 ◽  
Author(s):  
István Sebe ◽  
Eszter Ostorházi ◽  
Zsolt Bodai ◽  
Zsuzsanna Eke ◽  
József Szakács ◽  
...  
Keyword(s):  
Polyvinyl Alcohol ◽  
In Silico ◽  
Fibrous Scaffolds ◽  
Colistin Sulfate ◽  
Heat Treated ◽  
In Silico Characterization

Purification and characterization of moderately halophilic alkaline serine protease from marine Bacillus subtilis AP-MSU 6

2013 ◽  
Vol 2 (2) ◽  
pp. 116-119 ◽  
Author(s):  
Thirumalai Maruthiah ◽  
Palanichamy Esakkiraj ◽  
Ganesan Prabakaran ◽  
Arunachalam Palavesam ◽  
Grasian Immanuel
Keyword(s):  
Bacillus Subtilis ◽  
Serine Protease ◽  
Purification And Characterization ◽  
Alkaline Serine Protease ◽  
Moderately Halophilic
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