scholarly journals The effects of oral Aloe vera on the efficacy of transplanted human endothelial cells and the expression of matrix metalloproteinases in diabetic wound healing

Heliyon ◽  
2021 ◽  
pp. e08533
Author(s):  
Supassanan Kaewsrisung ◽  
Supakanda Sukpat ◽  
Nipan Issarasena ◽  
Suthiluk Patumraj ◽  
Juraiporn Somboonwong
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Matrix Metalloproteinases ◽  
Aloe Vera ◽  
Diabetic Wound ◽  
Human Endothelial Cells ◽  
Diabetic Wound Healing

scholarly journals The Role of Matrix Metalloproteinases in Diabetic Wound Healing in relation to Photobiomodulation

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Sandra Matabi Ayuk ◽  
Heidi Abrahamse ◽  
Nicolette Nadene Houreld
Keyword(s):  
Wound Healing ◽  
Matrix Metalloproteinases ◽  
Vascular Complications ◽  
Diabetic Patients ◽  
Main Function ◽  
Diabetic Wound ◽  
Impaired Wound Healing ◽  
Inflammatory Phase ◽  
Diabetic Wound Healing

The integration of several cellular responses initiates the process of wound healing. Matrix Metalloproteinases (MMPs) play an integral role in wound healing. Their main function is degradation, by removal of damaged extracellular matrix (ECM) during the inflammatory phase, breakdown of the capillary basement membrane for angiogenesis and cell migration during the proliferation phase, and contraction and remodelling of tissue in the remodelling phase. For effective healing to occur, all wounds require a certain amount of these enzymes, which on the contrary could be very damaging at high concentrations causing excessive degradation and impaired wound healing. The imbalance in MMPs may increase the chronicity of a wound, a familiar problem seen in diabetic patients. The association of diabetes with impaired wound healing and other vascular complications is a serious public health issue. These may eventually lead to chronic foot ulcers and amputation. Low intensity laser irradiation (LILI) or photobiomodulation (PBM) is known to stimulate several wound healing processes; however, its role in matrix proteins and diabetic wound healing has not been fully investigated. This review focuses on the role of MMPs in diabetic wound healing and their interaction in PBM.

scholarly journals Aloe Gel Enhances Angiogenesis in Healing of Diabetic Wound

2011 ◽  
Vol 3 (3) ◽  
pp. 204
Author(s):  
Djanggan Sargowo ◽  
Adeodatus Yuda Handaya ◽  
Mohammad Aris Widodo ◽  
Diana Lyrawati ◽  
Askandar Tjokroprawiro
Keyword(s):  
Nitric Oxide ◽  
Wound Healing ◽  
Wistar Rats ◽  
Aloe Vera ◽  
Diabetic Rats ◽  
Diabetic Wound ◽  
Aloe Vera Gel ◽  
Diabetic Wound Healing ◽  
Aloe Gel ◽  
Oxide Synthase

BACKGROUND: Diabetic micro and macroangiophathy lead to the incident of diabetic foot ulcers characterized by an increased number of circulating endothelial cells (CECs) and decreased function of endothelial progenitor cells (EPCs). This fact is correlated with ischemia and diabetic wound healing failure. Aloe vera gel is known to be able to stimulate vascular endothelial growth factor (VEGF) expression and activity by enhancing nitric oxide (NO) production as a result of nitric oxide synthase (NOS) enzyme activity. Aloe vera is a potential target to enhancing angiogenesis in wound healing.OBJECTIVE: The objective of this study was to explore the major role of Aloe vera gel in wound healing of diabetic ulcers by increasing the level of EPCs, VEGF, and endothelial nitric oxide synthase (eNOS), as well as by reducing the level of CECs involved in angiogenesis process of diabetic ulcers healing.METHODS: The experimental groups was divided into five subgroups consisting of non diabetic wistar rats, diabetic rats without oral administration of aloe gel, and treatment subgroup (diabetic rats) with 30, 60 and 120 mg/day of aloe gel doses for 14 days. All subgroups were wounded and daily observation was done on the wounds areas. Measurement of the number of EPCs (CD34), and CECs (CD45 and CD146) was done by flowcytometry, followed by measurement of VEGF and eNOS expression on dermal tissue by immunohistochemical method on day 0 and day 14 after treatment. The quantitative data were analyzed by One-Way ANOVA and Linear Regression, with a cofidence interval 5% and significance level (p<0.05) using SPSS 16 software to compare the difference and correlation between wound diameters, number of EPCs and CECs as well as the levels of VEGF and eNOS.RESULTS: The results of this study showed that aloe gel oral treatment in diabetic wistar rats was able to accelerate the wound healing process. It was shown by significant reduction of wound diameter (0.27±0.02); the increased number of CECs (0.42±0.57), respectively (p<0.05). On the other hand, the wound diameter and eNOS indicators showed significant differences at the dose of 60 mg, while the number of EPCs and CECs and the level of VEGF showed significantly different results at a dose of 120 mg. Aloe gel oral therapy showed a positive indication of wound healing acceleration at the optimum dose range 60-120 mg a day.CONCLUSIONS: Aloe gel is potential to be a herbal therapy candidate for diabetic wound healing through enhancing EPCs homing, decreasing the CECs number, and stimulating the increase of VEGF and eNOS levels,hence proving to be a dominant factor in the angiogenesis process.KEYWORDS: aloe gel, diabetes, wound healing, angiogenesis

scholarly journals Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects

2021 ◽  
Author(s):  
Xuejun Ni ◽  
Xiuying Shan ◽  
Lili Xu ◽  
Wenjun Yu ◽  
Mingliang Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Endothelial Cells ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Diabetic Wound ◽  
Closure Rate ◽  
Expression Levels ◽  
Sd Rats ◽  
Diabetic Wound Healing

Abstract Background: Wound healing is impaired in patients with diabetes due to the multifactorial etiology of the disease, which limits the therapeutic efficacy of various approaches. This study hypothesizes that the combination of adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) might achieve optimally efficient diabetic wound healing.Methods: ADSCs were isolated from the adipose tissues of Sprague-Dawley (SD) rats. PRP was prepared by using a two-step centrifugation technique. A diabetic wound model was established on the backs of SD rats to evaluate the effect of ADSCs incorporated into PRP. Hematoxylin and eosin staining, immunofluorescence and immunohistochemistry were performed to observe the changes in neovascularization. ELISA and Western blot were utilized to detect the angiogenesis-related protein expression levels. The proliferation of endothelial cells was assessed by the MTS assay.Results: ADSCs incorporated into PRP induced a higher wound closure rate than ADSCs, PRP and negative control. The expression levels of VEGF, p-STAT3 and SDF-1 in the ADSC-PRP group were higher than those in the other groups. Moreover, the proliferation of endothelial cells was strongly stimulated by treatment with the combination of ADSC-conditioned medium (ADSC-CM) and PRP.Conclusions: PRP enhanced diabetic wound healing induced by ADSCs, and its promoting effect involved neovascularization.

scholarly journals Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects

2020 ◽  
Author(s):  
Xuejun Ni ◽  
Xiuying Shan ◽  
Lili Xu ◽  
Wenjun Yu ◽  
Mingliang Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Endothelial Cells ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Diabetic Wound ◽  
Promoting Effect ◽  
Closure Rate ◽  
Sd Rats ◽  
Diabetic Wound Healing

Abstract Background: Wound healing is impaired in patients with diabetes due to the multifactorial etiology of the disease, which limits the therapeutic efficacy of various approaches. This study hypothesizes that the combination of adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) might achieve optimally efficient diabetic wound healing. Methods: ADSCs were isolated from the adipose tissues of Sprague-Dawley (SD) rats. PRP was prepared by using a two-step centrifugation technique. A diabetic wound model was established on the backs of SD rats to evaluate the effect of ADSCs incorporated into PRP. Hematoxylin and eosin staining, immunofluorescence and immunohistochemistry were performed to observe the changes in neovascularization. The proliferation of endothelial cells was assessed by the MTS assay. Results: ADSCs incorporated into PRP induced a higher wound closure rate than ADSCs, PRP and negative control. The expression levels of VEGF and SDF-1 in the ADSC-PRP group were higher than those in the other groups. Moreover, the proliferation of endothelial cells was strongly stimulated by treatment with the combination of ADSC-conditioned medium (ADSC-CM) and PRP. Conclusions: PRP enhanced diabetic wound healing induced by ADSCs, and its promoting effect involved neovascularization. Keywords: Adipose-derived stem cell, Platelet-rich plasma, Diabetic wound healing, Neovascularization.

scholarly journals Adipose-derived stem cells combined with platelet-rich plasma enhance wound healing in a rat model of full-thickness skin defects

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xuejun Ni ◽  
Xiuying Shan ◽  
Lili Xu ◽  
Wenjun Yu ◽  
Mingliang Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Endothelial Cells ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Diabetic Wound ◽  
Closure Rate ◽  
Expression Levels ◽  
Sd Rats ◽  
Diabetic Wound Healing

Abstract Background Wound healing is impaired in patients with diabetes due to the multifactorial etiology of the disease, which limits the therapeutic efficacy of various approaches. This study hypothesizes that the combination of adipose-derived stem cells (ADSCs) and platelet-rich plasma (PRP) might achieve optimally efficient diabetic wound healing. Methods ADSCs were isolated from the adipose tissues of Sprague-Dawley (SD) rats. PRP was prepared by using a two-step centrifugation technique. A diabetic wound model was established on the backs of SD rats to evaluate the effect of ADSCs incorporated into PRP. Hematoxylin and eosin staining, immunofluorescence, and immunohistochemistry were performed to observe the changes in neovascularization. ELISA and Western blot were utilized to detect the angiogenesis-related protein expression levels. The proliferation of endothelial cells was assessed by the MTS assay. Results ADSCs incorporated into PRP induced a higher wound closure rate than ADSCs, PRP, and negative control. The expression levels of VEGF, p-STAT3, and SDF-1 in the ADSC+PRP group were higher than those in the other groups. Moreover, the proliferation of endothelial cells was strongly stimulated by treatment with the combination of ADSC-conditioned medium (ADSC-CM) and PRP. Conclusions PRP enhanced diabetic wound healing induced by ADSCs, and its promoting effect involved neovascularization.

The diabetic wound healing effect of a two-herb formula and its mechanisms of action

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
CBS Lau ◽  
VKM Lau ◽  
CL Liu ◽  
PKK Lai ◽  
JCW Tam ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mechanisms Of Action ◽  
Diabetic Wound ◽  
Healing Effect ◽  
Diabetic Wound Healing ◽  
Wound Healing Effect

Critical Regulation of Angiogenesis by Nrf2 Signaling Is Absent in Diabetic Wound Healing

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 634-P
Author(s):  
PIUL S. RABBANI ◽  
JOSHUA A. DAVID ◽  
DARREN L. SULTAN ◽  
ALVARO P. VILLARREAL-PONCE ◽  
JENNIFER KWONG ◽  
...  
Keyword(s):  
Wound Healing ◽  
Diabetic Wound ◽  
Diabetic Wound Healing
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