scholarly journals Development of technology based on specific metabolic regulation of neurodegenerative disease (Research Center for Metabolic Regulation Neurodegenerative disease)

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S376
Author(s):  
Myeong Ok Kim ◽  
Myeong Ok Kim
Keyword(s):  
Neurodegenerative Disease ◽  
Metabolic Regulation ◽  
Research Center ◽  
Disease Research

scholarly journals A Simplified Brain Blocking Protocol Optimized for the Diagnosis of Neurodegenerative Disease Saves Time and Money While Preserving Anatomic Relationships

2020 ◽  
Author(s):  
Nathan F. Clement ◽  
John C. DeWitt ◽  
Matthew P. Frosch ◽  
Maria Martinez-Lage ◽  
Wesley R. Samore ◽  
...  
Keyword(s):  
Cost Effectiveness ◽  
Alzheimer Disease ◽  
Neurodegenerative Disease ◽  
Corticobasal Degeneration ◽  
Amyloid Angiopathy ◽  
Standard Protocol ◽  
Lewy Pathology ◽  
Disease Research ◽  
Sampling Protocols ◽  
Cerebral Amyloid

Context.— Postmortem evaluation for neurodegenerative disease is expensive in time and materials. These challenges can be met by implementing simpler sampling protocols while preserving anatomic relations. Objective.— To determine the diagnostic effectiveness and cost-effectiveness of a simplified brain blocking protocol compared with the standard blocking protocol used in our Alzheimer disease research center (ADRC). Design.— We prospectively compared the neuropathologic diagnoses established from our standard 19-cassette/19 brain sites ADRC protocol to a simplified 6-cassette/12 brain sites protocol in 52 consecutive cases. The simplified protocol generated 14 slides for comparison to 52 slides from our standard protocol. Results.— Compared with the ADRC protocol the simplified protocol produced Alzheimer Disease Neuropathologic Changes probability scores that were the same in 50 of 52 cases (r = 0.99). Staging for Lewy pathology was equivalent in 45 of 52 (r = 0.98), scoring for cerebral amyloid angiopathy was equivalent in 48 of 52 (r = 0.97), and grading for arteriolosclerosis was the same in 45 of 52 cases (r = 0.92). Progressive supranuclear palsy (n = 4), multiple system atrophy (n = 2), and corticobasal degeneration (n = 1) could be diagnosed by either protocol independently. The estimated savings per case was 72% or $1744.89 ($2436.37 [ADRC] versus $691.48 [simplified]). Conclusions.— The diagnosis of neurodegenerative disease at autopsy can be done accurately with a less expensive, simplified protocol. Our protocol is similar to those of previously published approaches, but it has a simpler organization scheme. This method should be valuable to institutions where autopsy cost considerations may be important.

scholarly journals Ontario Neurodegenerative Disease Research Initiative (ONDRI): Structural MRI Methods and Outcome Measures

2020 ◽  
Vol 11 ◽  
Author(s):  
Joel Ramirez ◽  
Melissa F. Holmes ◽  
Christopher J. M. Scott ◽  
Miracle Ozzoude ◽  
Sabrina Adamo ◽  
...  
Keyword(s):  
Neurodegenerative Disease ◽  
Outcome Measures ◽  
Structural Mri ◽  
Disease Research ◽  
Research Initiative

scholarly journals Human Autopsy-Derived Scalp Fibroblast Biobanking for Age-Related Neurodegenerative Disease Research

Cells ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 2383
Author(s):  
Suet Theng Beh ◽  
Carlye Frisch ◽  
David A. Brafman ◽  
Jared Churko ◽  
Jessica E. Walker ◽  
...  
Keyword(s):  
Neurodegenerative Disease ◽  
Disease Research ◽  
Body Tissues ◽  
Age Related ◽  
Neuropathological Diagnosis ◽  
Successful Establishment ◽  
Mrna Analysis ◽  
Induced Pluripotent ◽  
Procedure Development ◽  
Human Autopsy

The Arizona Study of Aging and Neurodegenerative Disorders/Brain and Body Donation Program at Banner Sun Health Research Institute (BSHRI) is a longitudinal clinicopathological study with a current enrollment of more than 900 living subjects for aging and neurodegenerative disease research. Annual clinical assessments are done by cognitive and movement neurologists and neuropsychologists. Brain and body tissues are collected at a median postmortem interval of 3.0 h for neuropathological diagnosis and banking. Since 2018, the program has undertaken banking of scalp fibroblasts derived from neuropathologically characterized donors with Alzheimer’s disease, Parkinson’s disease, and other neurodegenerative diseases. Here, we describe the procedure development and cell characteristics from 14 male and 15 female donors (mean ± SD of age: 83.6 ± 12.2). Fibroblasts from explant cultures were banked at passage 3. The results of mRNA analysis showed positive expression of fibroblast activation protein, vimentin, fibronectin, and THY1 cell surface antigen. We also demonstrated that the banked fibroblasts from a postmortem elderly donor were successfully reprogramed to human-induced pluripotent stem cells (hiPSCs). Taken together, we have demonstrated the successful establishment of a human autopsy-derived fibroblast banking program. The cryogenically preserved cells are available for request at the program website of the BSHRI.

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