Early mature bone formation using a bioactive membrane in dogs

Purpose: This study aimed to analyze the immunohistochemical effect of platelet-rich plasma (PRP) on healing of long-bone fractures in terms of bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF), the Ki-67 proliferation index, and radiological and histological analyses. Methods: Sixteen adult rabbits, whose right femoral diaphysis was fractured and fixed with Kirschner wires, were randomly divided into two groups, control and PRP (groups A and B, respectively). PRP was given to group B at 1 week postoperatively, and all animals were euthanized after 12 weeks. Radiographic evaluations were performed periodically. Cortical callus formation, chondroid and woven bone area percentages, osteoblastic and fibroblastic activities, and mature bone formation were examined. The depths of BMP-2 and VEGF staining were measured. The Ki-67 proliferation index was also calculated. Results: The mean radiological union score of group B was significantly higher than that of group A. There were also statistically significant differences between groups A and B in terms of cortical callus formation, woven bone area percentage, fibroblast proliferation, and mature bone formation. Group B had significantly more cortical callus and mature bone formation with less woven bone and fibroblast proliferation. Immunohistochemical analysis revealed no statistically significant difference between the groups in terms of BMP-2 and VEGF staining and the Ki-67 index. Conclusions: PRP had no effect on BMP-2 or VEGF levels with no increase in the Ki-67 proliferation index, although its application had a positive effect on bone healing by increasing callus and mature bone formation with decreased woven bone and fibroblast proliferation.

Download Full-text

Endometrial osseous metaplasia and mature bone formation with extramedullary hematopoiesis

Journal of Human Reproductive Sciences ◽

10.4103/0974-1208.82363 ◽

2011 ◽

Vol 4 (1) ◽

pp. 56 ◽

Cited By ~ 5

Author(s):

Prem Singh ◽

Kalyani Kapur ◽

Shveta Singla ◽

Navneet Naz

Keyword(s):

Bone Formation ◽

Extramedullary Hematopoiesis ◽

Osseous Metaplasia ◽

Mature Bone

Download Full-text

Ossified choroid plexus papilloma of the fourth ventricle: elucidation of the mechanism of osteogenesis in benign brain tumors

Journal of Neurosurgery Pediatrics ◽

10.3171/2013.3.peds12400 ◽

2013 ◽

Vol 12 (1) ◽

pp. 13-20 ◽

Cited By ~ 3

Author(s):

Sunil Manjila ◽

Erin Miller ◽

Amad Awadallah ◽

Shunichi Murakami ◽

Mark L. Cohen ◽

...

Keyword(s):

Brain Tumors ◽

Bone Formation ◽

Choroid Plexus ◽

Fourth Ventricle ◽

Choroid Plexus Papilloma ◽

Immunohistochemical Analysis ◽

Pluripotent Cell ◽

Bone Trabeculae ◽

Plexus Papilloma ◽

Mature Bone

True ossification within benign brain tumors is rare, and the molecular mechanism for this process is poorly understood. The authors report a case of ossified choroid plexus papilloma (CPP) and analyze it to help elucidate the underlying molecular basis of osteogenesis in benign brain tumors. A 21-year-old man presented with headache and depression that progressed over years. Computed tomography, MRI, and angiography demonstrated a large heavily calcified fourth ventricular tumor with a vascular blush and no hydrocephalus. The tumor was resected and was found to be an ossified CPP. Immunohistochemical staining for VEGF, Sox2, BMP-2, osterix, osteopontin, and osteocalcin was performed in an attempt to elucidate the mechanism of bone formation. The tumor was extensively ossified with mature bone trabeculae. Immunostaining for VEGF was positive. Additional staining showed the presence of osteocalcin in this ossified tumor but not in samples of nonossified CPPs collected from other patients. Staining for osterix and osteopontin was equivocally positive in the ossified CPP but also in the nonossified CPPs examined. The presence of osteocalcin in the ossified CPP demonstrates that there is true bone formation rather than simple calcification. Its appearance within cells around the trabeculae suggests the presence of osteoblasts. The presence of osterix suggests that a pluripotent cell, or one that is already partially differentiated, may be differentiated into an osteoblast through this pathway. This represents the first systematic immunohistochemical analysis of osteogenesis within choroid plexus tumors.

Download Full-text

Bone defect regeneration with bioactive glass implantation in rats

Journal of Applied Oral Science ◽

10.1590/s1678-77572004000200011 ◽

2004 ◽

Vol 12 (2) ◽

pp. 137-143 ◽

Cited By ~ 18

Author(s):

Nelson Luiz de Macedo ◽

Fábio da Silva Matuda ◽

Luís Guilherme Scavonne de Macedo ◽

Michel Borges Gonzales ◽

Suzana Martins Ouchi ◽

...

Keyword(s):

Bone Formation ◽

Bioactive Glass ◽

Bone Defects ◽

Male Rats ◽

New Bone Formation ◽

Osteoconductive Property ◽

Histological Processing ◽

Hematoxylin And Eosin ◽

Bone Trabeculae ◽

Mature Bone

The main goal of this study was to histologically evaluate the healing of surgically created defects on the tibiae of adult male rats after implantation of two types of bioactive glass. Sixteen adult Wistar rats (body weight of 300g) were divided into two groups: PerioGlas (PG) (n=8) and BioGran (BG) (n=8). Unicortical bone defects with 3-mm diameter were performed in both tibiae of the animals and filled with two types of glass particles. The rats were then sacrificed at 7, 14, 30 and 60 days, and the tissues were prepared for histological processing, sectioning, and staining with hematoxylin and eosin, as well as Mallory trichrome, and analyzed under light microscope. Within 7-14 days, both groups presented connective tissue septa with new bone formation, more intense in the PG group. In the subsequent periods (30 and 60 days), both groups presented more mature bone tissue around the glass particles. Bone trabeculae formed in all experimental periods were juxtaposed to the glass particles. It can be concluded that both materials promoted comparable bone formation over the entire extension of the defect, independently of the size of the granules, thus confirming their biological osteoconductive property.

Download Full-text

Evaluation of Cytotoxicity and Biocompatibility of Ti2AlC in Rabbits

Journal of Baghdad College of Dentistry ◽

10.26477/jbcd.v33i4.3015 ◽

2021 ◽

Vol 33 (4) ◽

pp. 20-24

Author(s):

Luma M Ibrahim ◽

Raghdaa K Jassim ◽

Ahmed Al Gabban

Keyword(s):

Bone Formation ◽

Dental Implant ◽

Progenitor Cell ◽

Histological Study ◽

Tissue Response ◽

Mtt Test ◽

Study Materials ◽

Viable Cells ◽

New Zealand White Rabbits ◽

Mature Bone

Background: The Titanium and its alloys are suitable for dental implant and medical applications. Biocompatibility of the materials is a major factor in determining the success of the implant and has a great impact on their rate of osseointegration. The aim of this study was to evaluate the biocompatibility and cytotoxicity of Ti2AlC in comparison to CPTi & Ti6Al7Nb in rabbits. Materials and Methods: 10 male New Zealand White rabbits, weighing (2-2.5 kg), aged (10-12 months) were used in this study. Cylindrical implants were prepared from the study materials (CPTi, Ti6Al7Nb and Ti2AlC) with (8mm) height and (3mm) diameter for the evaluation of tissue response and disc specimens were prepared with (6 mm) diameter and (2 mm) thickness for evaluation of cytotoxicity MTT test. A histological study was performed at 2 & 6 weeks post- surgical implant insertion. Results: Histological findings show that Ti2AlC has enhanced proliferation of osteo-progenitor cell and reported mature bone formation at 6 weeks. Moreover, Ti2AlC has recorded a higher percentage for viable cells by MTT test in comparison to CPTi and Ti6Al7Nb. Conclusion: The new Ti2AlC dental implant is considered biocompatible and has showed a better bone formation than the CPTi and Ti6Al7Nb materials at 2 & 6 weeks.

Download Full-text

Gastric gastrointestinal stromal tumor with osseous differentiation and stromal calcification: A case report and review of literature

SAGE Open Medical Case Reports ◽

10.1177/2050313x17746310 ◽

2017 ◽

Vol 5 ◽

pp. 2050313X1774631 ◽

Cited By ~ 3

Author(s):

Haneen Al-Maghrabi ◽

Abdelrazak Meliti

Keyword(s):

Case Report ◽

Bone Formation ◽

Gastrointestinal Stromal Tumor ◽

Gastrointestinal Stromal Tumors ◽

Gastric Wall ◽

Stromal Tumor ◽

Osseous Metaplasia ◽

Gastrointestinal Malignancies ◽

Stromal Tumors ◽

Mature Bone

Gastrointestinal stromal tumors are the most common primary mesenchymal tumors of the gastrointestinal tract accounting for 0.1%–3.0% of all gastrointestinal malignancies. The stomach is the most common site (60%) followed by the small bowel (30%–35%) particularly jejunum and ileum, colorectum (5%) and rarely affect esophagus and appendix. Most gastrointestinal stromal tumors arise sporadically, however, less commonly they develop in association with various clinical syndromes like Carney triad, Carney–Stratakis syndrome, familial gastrointestinal stromal tumor syndrome and neurofibromatosis type1 (NF1). We report a 65-year-old male patient presented with gastric mass (7.5 × 4.5 × 3.5 cm) arising from the posterior gastric wall. Histologic examination revealed neoplastic proliferation of spindled and epithelioid cells with focal plexiform pattern and low mitotic activity 3/50 HPF. No evidence of cytological atypia, abnormal mitosis or necrosis. Interestingly enough, there were focal areas of mature bone formation/osseous differentiation associated with calcification. The tumor cells were strongly positive for CD117, DOG1 with focal immunoreactivity against CD34. The morphologic features and the immunoprofile were diagnostic of gastrointestinal stromal tumor. Herein, we present a rare case of gastric gastrointestinal stromal tumor with mature bone formation, osseous metaplasia and calcification. To the best of our knowledge, this is the second case report of gastric gastrointestinal stromal tumor with osseous differentiation and mature bone formation.

Download Full-text