scholarly journals Evaluation of Cytotoxicity and Biocompatibility of Ti2AlC in Rabbits

2021 ◽  
Vol 33 (4) ◽  
pp. 20-24
Author(s):  
Luma M Ibrahim ◽  
Raghdaa K Jassim ◽  
Ahmed Al Gabban
Keyword(s):  
Bone Formation ◽  
Dental Implant ◽  
Progenitor Cell ◽  
Histological Study ◽  
Tissue Response ◽  
Mtt Test ◽  
Study Materials ◽  
Viable Cells ◽  
New Zealand White Rabbits ◽  
Mature Bone

Background: The Titanium and its alloys are suitable for dental implant and medical applications. Biocompatibility of the materials is a major factor in determining the success of the implant and has a great impact on their rate of osseointegration. The aim of this study was to evaluate the biocompatibility and cytotoxicity of Ti2AlC in comparison to CPTi & Ti6Al7Nb in rabbits. Materials and Methods: 10 male New Zealand White rabbits, weighing (2-2.5 kg), aged (10-12 months) were used in this study. Cylindrical implants were prepared from the study materials (CPTi, Ti6Al7Nb and Ti2AlC) with (8mm) height and (3mm) diameter for the evaluation of tissue response and disc specimens were prepared with (6 mm) diameter and (2 mm) thickness for evaluation of cytotoxicity MTT test. A histological study was performed at 2 & 6 weeks post- surgical implant insertion. Results: Histological findings show that Ti2AlC has enhanced proliferation of osteo-progenitor cell and reported mature bone formation at 6 weeks. Moreover, Ti2AlC has recorded a higher percentage for viable cells by MTT test in comparison to CPTi and Ti6Al7Nb. Conclusion: The new Ti2AlC dental implant is considered biocompatible and has showed a better bone formation than the CPTi and Ti6Al7Nb materials at 2 & 6 weeks.

scholarly journals Acceleration of Bone Formation and Adhesion Ability on Dental Implant Surface via Plasma Electrolytic Oxidation in a Solution Containing Bone Ions

Metals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 106
Author(s):  
Mosab Kaseem ◽  
Han-Cheol Choe
Keyword(s):  
Bone Formation ◽  
Dental Implant ◽  
Plasma Electrolytic Oxidation ◽  
Adhesion Ability ◽  
Post Surgery ◽  
Dental Implant Surface ◽  
Significant Difference ◽  
Rabbit Tibia ◽  
Electrolytic Oxidation ◽  
Plasma Electrolytic

The present study examined the in vitro and in vivo bone formation and adhesion ability on the surface of a titanium dental implant made by plasma electrolytic oxidation (PEO) in electrolytes containing bioactive ions. To achieve this goal, screw-shaped fabricated Ti-6Al-4V alloy implants were processed via PEO using an electrolyte solution containing calcium (Ca), phosphorous (P), magnesium (Mg), zinc (Zn), strontium (Sr), silicon (Si), and manganese (Mn) species. The screw implants doped with bioactive elements via PEO were placed in rabbit tibia, and the results were compared to the sand-blasted Ti-6Al-4V alloy implants. At eight-week post-surgery, there was no significant difference in the values of removal torque between sand-blasted and PEO-treated implants. However, it was observed that the PEO treatment of dental implants led to the formation of more periphery bone as compared to the case of sand-blasted implants. Accordingly, the PEO-treated implants have the potential to be used as promising materials for dental applications.

Tissue Response of Chick Chorioallantoic Membranes to Dental Implant Materials

1966 ◽  
Vol 45 (3) ◽  
pp. 900-906 ◽  
Author(s):  
J.C. Thonard ◽  
R.F. Sisca ◽  
W.A. George ◽  
D.A. Lower
Keyword(s):  
Dental Implant ◽  
Tissue Response ◽  
Implant Materials

scholarly journals Experiments on skeletal growth and development in vitro in relation to the problem of avian phokomelia

1935 ◽  
Vol 118 (807) ◽  
pp. 133-154 ◽  
Keyword(s):  
Bone Formation ◽  
Bone Growth ◽  
Histological Study ◽  
Detailed Comparison ◽  
Specific Gene ◽  
Body Parts ◽  
Continue Development ◽  
Hatching Time ◽  
Stage Of Development

The experiments to be reported in the following pages were suggested by observations made by one of us on the so-called Creeper fowl. Creeper chickens are characterized by a disproportionate shortness of the long bones of the extremities. Histological study has shown that Creeper chickens belong in the same category as the disproportionate dwarfism of mammals known as chondrodystrophy or achondroplasia (Landauer, 1931) . The Creeper characters are inherited as a Mendelian dominant and are lethal in homozygous condition (Landauer and Dunn, 1930). Homozygous Creeper embryos generally die after about 72 hours of incubation, but in rare cases they survive beyond this stage and continue development up to nearly hatching time. These late stages of homozygous Creeper embryos exhibit striking malformations of the extremities which are known as phokomelia (Landauer, 1933). A study of the early embryonic development of homozygous Creeper embryos (Landauer, 1932) led to the conclusion that the effects of the Creeper mutation are not brought about by specific gene action on those body parts which later show deformities, but by a general retardation of body growth at a definite stage of development. This conclusion was strengthened by a detailed comparison of embryonic and post-natal bone growth in heterozygous Creeper and normal chickens (Landauer, 1934). All evidence which so far has been obtained in this work points to the conclusion that the characteristic traits of heterozygous as well as homozygous Creeper chicks are produced by an unspecific retardation of development at a time when formation of the buds of the extremities (and of the head which in homozygous embryos also shows deformities later on) are proceeding at a particularly rapid rate, thereby causing specific disturbances in the differentiation of these parts. It seemed to us that it should be possible to put these conclusions to an experimental test. The most promising way of approach appeared to be an attempt to produce in vitro the extreme abnormalities of bone formation shown by the extremities of phokomelic homozygous Creeper embryos. These abnormalities chiefly consist in (1) a general retardation of cartilage differentiation; (2) lack of bone formation; and (3) frequent partial fusion of ulna and radius on the one hand, tibia and fibula on the other, or presence of only one bone in these segments instead of two.

Tissue Reaction to Subcutaneous Implantation of SuperEBA (Reinforced Zinc Oxide Cement) Used as Root-End Filling Material: A Histological Study in Rats

2016 ◽  
Vol 695 ◽  
pp. 247-251
Author(s):  
Alexandru Andrei Iliescu ◽  
Cristian Marian Petcu ◽  
Ileana Cristiana Petcu ◽  
Irina Maria Gheorghiu ◽  
Andrei Iliescu ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Histological Study ◽  
Tissue Reaction ◽  
Tissue Response ◽  
Successful Outcome ◽  
Filling Material ◽  
Clinical Value ◽  
Filling Materials ◽  
Retrograde Filling ◽  
Specific Tissue

The retrograde filling is a critical step to a successful outcome of the endodontic surgery. Despite the progress in the technology of novel root-end filling materials, zinc oxide-eugenol cement superEBA is still preserving its clinical value on long-term basis. The study aimed to reconsider the tissue response to the initial irritating effect of this material. Silicon tubes filled with superEBA were subcutaneously implanted for 120 days in white Wistar rats which were afterwards sacrificed. The connective tissue surrounding the superEBA implants revealed fibroblast proliferation and a definite reparatory process without inflammatory reaction. A non-specific tissue healing in progress around the implants, without calcifications, necrosis, and apoptosis was also described after 4 months. SuperEBA proved on animal model that its cytotoxicity is reducing gradually in time until no adverse reaction is observed. The reduced content in eugenol compared to other surgical zinc oxide cements and the benefic effect of o-ethoxybenzoic acid are the support to reconsider SuperEBA as a biocompatible retrograde filling material.

Tissue Engineering of Bone by Osteoinductive Biomaterials

MRS Bulletin ◽  
1996 ◽  
Vol 21 (11) ◽  
pp. 36-39 ◽  
Author(s):  
Ugo Ripamonti ◽  
Nicolaas Duneas
Keyword(s):  
Tissue Engineering ◽  
Bone Formation ◽  
Type I Collagen ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Mesenchymal Cells ◽  
Tissue Response ◽  
Type I ◽  
New Bone Formation ◽  
Demineralized Bone

Recent advances in materials science and biotechnology have given birth to the new and exciting field of tissue engineering, in which the two normally disparate fields are merging into a profitable matrimony. In particular the use of biomaterials capable of initiating new bone formation via a process called osteoinduction is leading to quantum leaps for the tissue engineering of bone.The classic work of Marshall R. Urist and A. Hari Reddi opened the field of osteoinductive biomaterials. Urist discovered that, upon implantation of devitalized, demineralized bone matrix in the muscle of experimental animals, new bone formation occurs within two weeks, a phenomenon he described as bone formation by induction. The tissue response elicited by implantation of demineralized bone matrix in muscle or under the skin includes activation and migration of undifferentiated mesenchymal cells by chemotaxis, anchoragedependent cell attachment to the matrix, mitosis and proliferation of mesenchymal cells, differentiation of cartilage, mineralization of the cartilage, vascular invasion of the cartilage, differentiation of osteoblasts and deposition of bone matrix, and finally mineralization of bone and differentiation of marrow in the newly developed ossicle.The osteoinductive ability of the extracellular matrix of bone is abolished by the dissociative extraction of the demineralized matrix, but is recovered when the extracted component, itself inactive, is reconstituted with the inactive residue—mainly insoluble collagenous bone matrix. This important experiment showed that the osteoinductive signal resides in the solubilized component but needs to be reconstituted with an appropriate carrier to restore the osteoinductive activity. In this case, the carrier is the insoluble collagenous bone matrix—mainly crosslinked type I collagen.

scholarly journals Physical and Histological Comparison of Hydroxyapatite, Carbonate Apatite, and β-Tricalcium Phosphate Bone Substitutes

Materials ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 1993 ◽  
Author(s):  
Kunio Ishikawa ◽  
Youji Miyamoto ◽  
Akira Tsuchiya ◽  
Koichiro Hayashi ◽  
Kanji Tsuru ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Defect ◽  
Tricalcium Phosphate ◽  
Bone Substitutes ◽  
Physiological Solution ◽  
Tissue Response ◽  
Histological Evaluation ◽  
Carbonate Apatite ◽  
Artificial Bone ◽  
New Bone Formation

Three commercially available artificial bone substitutes with different compositions, hydroxyapatite (HAp; Neobone®), carbonate apatite (CO3Ap; Cytrans®), and β-tricalcium phosphate (β-TCP; Cerasorb®), were compared with respect to their physical properties and tissue response to bone, using hybrid dogs. Both Neobone® (HAp) and Cerasorb® (β-TCP) were porous, whereas Cytrans® (CO3Ap) was dense. Crystallite size and specific surface area (SSA) of Neobone® (HAp), Cytrans® (CO3Ap), and Cerasorb® (β-TCP) were 75.4 ± 0.9 nm, 30.8 ± 0.8 nm, and 78.5 ± 7.5 nm, and 0.06 m2/g, 18.2 m2/g, and 1.0 m2/g, respectively. These values are consistent with the fact that both Neobone® (HAp) and Cerasorb® (β-TCP) are sintered ceramics, whereas Cytrans® (CO3Ap) is fabricated in aqueous solution. Dissolution in pH 5.3 solution mimicking Howship’s lacunae was fastest in CO3Ap (Cytrans®), whereas dissolution in pH 7.3 physiological solution was fastest in β-TCP (Cerasorb®). These results indicated that CO3Ap is stable under physiological conditions and is resorbed at Howship’s lacunae. Histological evaluation using hybrid dog mandible bone defect model revealed that new bone was formed from existing bone to the center of the bone defect when reconstructed with CO3Ap (Cytrans®) at week 4. The amount of bone increased at week 12, and resorption of the CO3Ap (Cytrans®) was confirmed. β-TCP (Cerasorb®) showed limited bone formation at week 4. However, a larger amount of bone was observed at week 12. Among these three bone substitutes, CO3Ap (Cytrans®) demonstrated the highest level of new bone formation. These results indicate the possibility that bone substitutes with compositions similar to that of bone may have properties similar to those of bone.

scholarly journals BMSCs Seeding in Different Scaffold Incorporation with Hyperbaric Oxygen Treat Seawater Immersed Bony Defect

2021 ◽  
Author(s):  
gan zhang ◽  
Xiaosong Chen ◽  
Xunsheng Cheng ◽  
Xiuwu Ma ◽  
Congcong Chen
Keyword(s):  
Bone Formation ◽  
Bone Defect ◽  
Medullary Canal ◽  
Bony Defect ◽  
New Bone Formation ◽  
X Ray ◽  
New Zealand White Rabbits ◽  
Group A ◽  
Group B ◽  
Group D

Abstract Introduction: The experiment was undertaken to estimate the effect of BMSCs seeding in different scaffold incorporation with HBO on the repair of seawater immersed bone defect. And future compared n-HA/PLGA with β-TCP/PLGA as scaffold in treatment effect of seawater immersed bone defect.Methods: 60 New Zealand White rabbits with standard seawater defect in radius were randomly divided to group A (implant with nothing), group B (implanted with atuogenous bone), group C (implanted with n-HA/PLGA/BMSCs, and Group D ( implanted with β-TCP/PLGA/BMSCs). After implant, each rabbit receive HBO treatment at 2.4 ATA 100% oxygen for 120 minutes per day for 2 weeeks. Radiograph, histological and biomechanical examination were used to analyze osteogenesis.Result: X-ray analysis show that n-HA/PLGA/BMSCs and β-TCP/PLGA/BMSCs could accelerate the new bone formation, and the new bone formation in group C was lager than in group D or group A, and close to group B (P<0.05). After 12 weeks, in group A, defect without scaffold show a loose connect tissue filled in the areas. The medullary canal in group B was recanalizated. Defect in group C and D show a larger number of wove bone formation. The new wove bone formation in defect areas in group C was lager than D. The mechanical examination revealed ultimate strength at 12 weeks were group D>group C>group B>group A(P<0.05).Conclusion: Scaffold of n-HA/PLGA and β-TCP/PLGA incorporation with HBO and BMSCs were effective to treat seawater immersed bone defect, and n-HA/PLGA was more excellent than β-TCP/PLGA.

A histological study of tissue response to simulated cleft palate surgery at different ages in Beagle dogs

1991 ◽  
Vol 36 (11) ◽  
pp. 837-843 ◽  
Author(s):  
M.G.M.M. Wijdeveld ◽  
J.C. Maltha ◽  
E.M. Grupping ◽  
J. De Jonge ◽  
A.M. Kuijpers-Jagtman
Keyword(s):  
Cleft Palate ◽  
Histological Study ◽  
Tissue Response ◽  
Beagle Dogs ◽  
Different Ages

scholarly journals Platelet-rich plasma decreases fibroblastic activity and woven bone formation with no significant immunohistochemical effect on long-bone healing: An experimental animal study with radiological outcomes

2018 ◽  
Vol 26 (3) ◽  
pp. 230949901880249 ◽  
Author(s):  
İbrahim Deniz Canbeyli ◽  
Rahmi Can Akgun ◽  
Orcun Sahin ◽  
Aysen Terzi ◽  
İsmail Cengiz Tuncay
Keyword(s):  
Bone Formation ◽  
Callus Formation ◽  
Platelet Rich Plasma ◽  
Long Bone ◽  
Proliferation Index ◽  
Fibroblast Proliferation ◽  
Ki 67 ◽  
Woven Bone ◽  
Group B ◽  
Mature Bone

Purpose: This study aimed to analyze the immunohistochemical effect of platelet-rich plasma (PRP) on healing of long-bone fractures in terms of bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF), the Ki-67 proliferation index, and radiological and histological analyses. Methods: Sixteen adult rabbits, whose right femoral diaphysis was fractured and fixed with Kirschner wires, were randomly divided into two groups, control and PRP (groups A and B, respectively). PRP was given to group B at 1 week postoperatively, and all animals were euthanized after 12 weeks. Radiographic evaluations were performed periodically. Cortical callus formation, chondroid and woven bone area percentages, osteoblastic and fibroblastic activities, and mature bone formation were examined. The depths of BMP-2 and VEGF staining were measured. The Ki-67 proliferation index was also calculated. Results: The mean radiological union score of group B was significantly higher than that of group A. There were also statistically significant differences between groups A and B in terms of cortical callus formation, woven bone area percentage, fibroblast proliferation, and mature bone formation. Group B had significantly more cortical callus and mature bone formation with less woven bone and fibroblast proliferation. Immunohistochemical analysis revealed no statistically significant difference between the groups in terms of BMP-2 and VEGF staining and the Ki-67 index. Conclusions: PRP had no effect on BMP-2 or VEGF levels with no increase in the Ki-67 proliferation index, although its application had a positive effect on bone healing by increasing callus and mature bone formation with decreased woven bone and fibroblast proliferation.

Marrow-derived Progenitor Cell Injections Enhance New Bone Formation during Distraction

2000 ◽  
Vol 82 (4) ◽  
pp. 104
Author(s):  
Mark Richards ◽  
Barbara A. Huibregtse ◽  
Arnold I. Caplan ◽  
James A. Goulet ◽  
Steven A. Goldstein
Keyword(s):  
Bone Formation ◽  
Progenitor Cell ◽  
New Bone Formation
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