Performance of MicroScan Overnight Panels for antibiotic susceptibility testing of staphylococci strains directly from positive blood culture bottles

The existing identification (ID) and antimicrobial susceptibility testing (AST) method requires at least two to three days to detect blood infection, and a fast and accurate detection method is very necessary for sepsis patients or Intensive Care Unit (ICU) patients. Here, we describe a direct isolated bacteria from a positive blood culture bottle (PBCB), and rapid AST method by femtosecond stimulated Raman scattering (SRS) imaging of deuterium oxide (D2O) metabolism, which can determine the antimicrobial susceptibility of bacteria from PBCB in 5-6 hours. The positive blood culture sample is passed through a filter membrane and mixed with cell lysis, after through the centrifugal which can directly isolated bacterium in order to identification by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS), following by antibiotic susceptibility testing by SRS imaging within a day. Overall, this rapid and rapid process combination of MALDI-TOF MS and SRS imaging of deuterium oxide (D2O) metabolism can solve the direct identification and antibiotic susceptibility testing of pathogen in positive blood cultures.

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Comparative evaluation of the QMAC-dRAST V2.0 system for rapid antibiotic susceptibility testing of Gram-negative blood culture isolates

Journal of Microbiological Methods ◽

10.1016/j.mimet.2020.105902 ◽

2020 ◽

Vol 172 ◽

pp. 105902

Author(s):

Patrick Grohs ◽

Emilie Rondinaud ◽

Myriam Fourar ◽

Karama Rouis ◽

Jean-Luc Mainardi ◽

...

Keyword(s):

Blood Culture ◽

Antibiotic Susceptibility ◽

Comparative Evaluation ◽

Susceptibility Testing ◽

Antibiotic Susceptibility Testing ◽

Negative Blood Culture ◽

Gram Negative

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144. Organism Identification and Antibiotic Susceptibilities with Verigene Blood Culture Assay: a Retrospective Single-Center Study

Open Forum Infectious Diseases ◽

10.1093/ofid/ofz360.219 ◽

2019 ◽

Vol 6 (Supplement_2) ◽

pp. S99-S100

Author(s):

Stephanie Tchen ◽

Steven Smoke ◽

Maria DeVivo

Keyword(s):

Blood Culture ◽

Antibiotic Susceptibility ◽

Susceptibility Testing ◽

Medical Center ◽

Academic Medical Center ◽

Blood Cultures ◽

Molecular Testing ◽

Antibiotic Susceptibility Testing ◽

Resistance Determinants ◽

Organism Identification

Abstract Background The Verigene blood culture assay is a rapid molecular testing platform for positive blood cultures. Verigene detects a limited number of bacteria and a limited number of antibiotic resistance determinants. While certain Verigene results have clear implications for optimal antibiotic therapy prior to complete antibiotic susceptibility testing, others do not. The purpose of this study was to compare the results of the Verigene blood culture assay with standard organism identification and antibiotic susceptibility testing. Methods This was a retrospective cohort study conducted at a single academic medical center. The study period was 14 months from November 2017 to December 2018. All Verigene results from the study period were reviewed and compared with the results of standard organism identification and susceptibility testing. Organism identification and antibiotic susceptibility testing were performed by Vitek MS and Vitek 2. Duplicate results from the same patient were excluded. The primary outcome was the percentage of blood cultures correctly identified by Verigene. Secondary outcomes included the antibiotic susceptibility of organisms identified by Verigene in the presence and absence of resistance determinants and the identity and frequency of organisms not detected by Verigene. Results A total of 782 Verigene results were screened. After exclusions, 675 Verigene results including 737 organisms from 597 patients were included. Of 737 organisms, Verigene correctly identified 611 (82.9%), incorrectly identified 19 (2.6%) and was unable to identify 107 (14.5%) off-panel organisms. Tables 1 and 2 outline the antibiotic susceptibility of organisms by the presence or absence of resistance determinants in Gram-negative and Gram-positive bacteria, respectively. Table 3 describes the identities of the organisms not detected by Verigene, stratified by Gram stain result. Conclusion The Verigene blood culture assay demonstrated accuracy in identifying organisms and predicting antibiotic susceptibility. These results will help inform the prospective interpretation of Verigene results and subsequent antibiotic selection at the study institution. Disclosures All authors: No reported disclosures.

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Rapid, Direct Antibiotic Susceptibility Testing of Blood Culture Isolates Using the Abbott Avantage® System

American Journal of Clinical Pathology ◽

10.1093/ajcp/86.5.665 ◽

1986 ◽

Vol 86 (5) ◽

pp. 665-669 ◽

Cited By ~ 3

Author(s):

Frederick S. Nolte ◽

Paul B. Contestable ◽

Durry Lincalis ◽

Amado Punsalang

Keyword(s):

Blood Culture ◽

Antibiotic Susceptibility ◽

Susceptibility Testing ◽

Antibiotic Susceptibility Testing

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Rapid and accurate direct antibiotic susceptibility testing of blood culture broths using MALDI Sepsityper combined with the BD Phoenix automated system

Journal of Medical Microbiology ◽

10.1099/jmm.0.075580-0 ◽

2014 ◽

Vol 63 (12) ◽

pp. 1590-1594 ◽

Cited By ~ 18

Author(s):

Briony Hazelton ◽

Lee C. Thomas ◽

Thomas Olma ◽

Jen Kok ◽

Matthew O’Sullivan ◽

...

Keyword(s):

Blood Culture ◽

Antibiotic Susceptibility ◽

Susceptibility Testing ◽

Turnaround Time ◽

Automated System ◽

Antibiotic Susceptibility Testing ◽

Gram Negative ◽

Cell Pellet ◽

Novel Method ◽

Susceptibility Tests

Antibiotic susceptibility testing with the BD Phoenix system on bacterial cell pellets generated from blood culture broths using the Bruker MALDI Sepsityper kit was evaluated. Seventy-six Gram-negative isolates, including 12 with defined multi-resistant phenotypes, had antibiotic susceptibility testing (AST) performed by Phoenix on the cell pellet in parallel with conventional methods. In total, 1414/1444 (97.9 %) of susceptibility tests were concordant, with only 1 (0.07 %) very major error. This novel method has the potential to reduce the turnaround time for AST results by up to a day for Gram-negative bacteraemias.

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