Hyaluronan microenvironment enhances cartilage regeneration of human adipose-derived stem cells in a chondral defect model

2018 ◽  
Vol 119 ◽  
pp. 726-740 ◽  
Author(s):  
Shun-Cheng Wu ◽  
Pei-Yi Huang ◽  
Chung-Hwan Chen ◽  
Benjamin Teong ◽  
Jhen-Wei Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cartilage Regeneration ◽  
Adipose Derived Stem Cells ◽  
Chondral Defect ◽  
Defect Model

scholarly journals Assessment of human cartilage regeneration in a patient with knee osteoarthritis using autologous adipose-tissue-derived stem cells and Platelet-rich plasma: a case study

2020 ◽  
pp. 73-78
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Physical Therapy ◽  
Platelet Rich Plasma ◽  
Cartilage Regeneration ◽  
Adipose Derived Stem Cells ◽  
Human Cartilage ◽  
Percutaneous Injection ◽  
Knee Oa ◽  
Mri Scans

Cartilage regenerative medicine has been met with much interest due to their ability to inhibit disease progression of osteoarthritis (OA). The use of adipose-derived stem cells has been suggested as a reliable method for OA treatment because of their potential to differentiate into a variety of cell lines and their potent capability to self-renewal and repair. The aim of this study is to assess adipose-derived stem cells in combination with PRP ability in treating a patient with knee OA. A 53-year- old man with osteoarthritis was selected for this treatment. Human abdominal subcutaneous adipose sample was obtained from a patient with knee OA. Stem cells were obtained from adipose tissue of abdominal origin by digesting lipoaspirate tissue with collagenase. ADSCs cultured in DMEM medium supplemented with 10% FBS. Also, ADSCs expanded and characterized by flow cytometry. These stem cells, along with platelet-rich plasma and calcium chloride, were injected into the right knee. Pre-treatment and post-treatment MRI scans, physical therapy, and pain score data were then analyzed. The MRI data for the patient demonstrated significant positive changes. Probable cartilage regeneration was sensible in the patient. Along with MRI evidence, the measured physical therapy outcomes, subjective pain, and functional status all improved. Autologous adipose-derived stem cell injection, in conjunction with platelet-rich plasma is a promising minimally invasive therapy for osteoarthritis of human knees. The present clinical case report demonstrated that a combination of percutaneous injection of autologous ADSCs and PRPmay be able to regenerate cartilage in human knee OA.

scholarly journals Auricular Cartilage Regeneration with Adipose-Derived Stem Cells in Rabbits

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Se-Joon Oh ◽  
Hee-Young Park ◽  
Kyung-Un Choi ◽  
Sung-Won Choi ◽  
Sung-Dong Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cartilage Defect ◽  
Collagen Type ◽  
Cartilage Regeneration ◽  
Control Group ◽  
Adipose Derived Stem Cells ◽  
Type Ii ◽  
Collagen Type Ii ◽  
Auricular Cartilage

Tissue engineering cell-based therapy using induced pluripotent stem cells and adipose-derived stem cells (ASCs) may be promising tools for therapeutic applications in tissue engineering because of their abundance, relatively easy harvesting, and high proliferation potential. The purpose of this study was to investigate whether ASCs can promote the auricular cartilage regeneration in the rabbit. In order to assess their differentiation ability, ASCs were injected into the midportion of a surgically created auricular cartilage defect in the rabbit. Control group was injected with normal saline. After 1 month, the resected auricles were examined histopathologically and immunohistochemically. The expression of collagen type II and transforming growth factor-β1 (TGF-β1) were analyzed by quantitative polymerase chain reaction. Histopathology showed islands of new cartilage formation at the site of the surgically induced defect in the ASC group. Furthermore, Masson’s trichrome staining and immunohistochemistry for S-100 showed numerous positive chondroblasts. The expression of collagen type II and TGF-β1 were significantly higher in the ASCs than in the control group. In conclusion, ASCs have regenerative effects on the auricular cartilage defect of the rabbit. These effects would be expected to contribute significantly to the regeneration of damaged cartilage tissue in vivo.

scholarly journals Differentiation of adipose-derived stem cells into functional chondrocytes by a small molecule that induces Sox9

2020 ◽  
Vol 52 (4) ◽  
pp. 672-681 ◽  
Author(s):  
Jiyun Lee ◽  
Chang Youn Lee ◽  
Jun-Hee Park ◽  
Hyang-Hee Seo ◽  
Sunhye Shin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Small Molecule ◽  
Cartilage Regeneration ◽  
Hypertrophic Chondrocytes ◽  
Joint Disease ◽  
Chondrocyte Differentiation ◽  
Adipose Derived Stem Cells ◽  
Key Factor

Abstract Osteoarthritis (OA) is a common joint disease that results from the disintegration of joint cartilage and the underlying bone. Because cartilage and chondrocytes lack the ability to self-regenerate, efforts have been made to utilize stem cells to treat OA. Although various methods have been used to differentiate stem cells into functional chondrocytes, the currently available methods cannot induce stem cells to undergo differentiation into chondrocyte-like cells without inducing characteristics of hypertrophic chondrocytes, which finally lead to cartilage disintegration and calcification. Therefore, an optimized method to differentiate stem cells into chondrocytes that do not display undesired phenotypes is needed. This study focused on differentiating adipose-derived stem cells (ASCs) into functional chondrocytes using a small molecule that regulated the expression of Sox9 as a key factor in cartilage development and then explored its ability to treat OA. We selected ellipticine (ELPC), which induces chondrocyte differentiation of ASCs, using a GFP-Sox9 promoter vector screening system. An in vivo study was performed to confirm the recovery rate of cartilage regeneration with ASC differentiation into chondrocytes by ELPC in a collagenase-induced animal model of OA. Taken together, these data indicate that ellipticine induces ASCs to differentiate into mature chondrocytes without hypertrophic chondrocytes in vitro and in vivo, thus overcoming a problem encountered in previous studies. These results indicate that ELPC is a novel chondrocyte differentiation-inducing drug that shows potential as a cell therapy for OA.

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