Silica nanoparticles induce conformational changes of tau protein and oxidative stress and apoptosis in neuroblastoma cell line

2019 ◽  
Vol 124 ◽  
pp. 1312-1320 ◽  
Author(s):  
Zahra Roshanfekrnahzomi ◽  
Paria Badpa ◽  
Behnaz Esfandiari ◽  
Saba Taheri ◽  
Mina Nouri ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Silica Nanoparticles ◽  
Cell Line ◽  
Conformational Changes ◽  
Tau Protein ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
And Oxidative Stress

Probing the interaction of silver nanoparticles with tau protein and neuroblastoma cell line as nervous system models

2017 ◽  
Vol 36 (15) ◽  
pp. 4057-4071 ◽  
Author(s):  
Sara Rahmani ◽  
Leila Mogharizadeh ◽  
Farnoosh Attar ◽  
Seyed Mahdi Rezayat ◽  
Seyyedeh Elaheh Mousavi ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Nervous System ◽  
Cell Line ◽  
Tau Protein ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
System Models

scholarly journals HIV Protease Inhibitors Apoptotic Effect in SH-SY5Y Neuronal Cell Line

2016 ◽  
Vol 39 (4) ◽  
pp. 1463-1470 ◽  
Author(s):  
Paola Maura Tricarico ◽  
Rafael Freitas de Oliveira Franca ◽  
Sabrina Pacor ◽  
Valentina Ceglia ◽  
Sergio Crovella ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Line ◽  
Protease Inhibitors ◽  
Heme Oxygenase ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
Hiv Protease ◽  
Hiv Protease Inhibitors ◽  
Cellular Models ◽  
The Impact

Background: Prophylactic treatment regimens to prevent mother-to-child HIV transmission include protease inhibitors Lopinavir and Ritonavir. Lopinavir and Ritonavir have been reported to be able to induce intracellular oxidative stress in diverse cellular models, however scarce informations are available about protease inhibitor effects of in the central nervous system (CNS). In our study we evaluated the impact of protease inhibitors on a cell neuronal model. Methods: We treated a neuroblastoma cell line (SH-SY5Y) with increasing doses of Lopinavir and Ritonavir (0.1-1-10-25-50 µM), used alone or in combination, evaluating the impact of these drugs in terms of mitochondrial activity, with MTT cell proliferation assay; mRNA expression of heme oxygenase (HemeOH) and reactive oxygen species (ROS) levels with 2',7'-dichlorofluorescin diacetate (H2DCFDA) in order to assess oxidative stress; apoptotic cell death with flow cytometry. Results: We observed that Lopinavir and Ritonavir treatment, at 25 and/or 50 µM concentrations, induced mitochondrial damage, increase of heme oxygenase RNA expression levels and ROS generation, followed by apoptosis in SH-SY5Y. Conclusions: Our in vitro model demonstrates a damaging effect of HIV protease inhibitors on the neuroblastoma cell line, thus partially mimicking the impact of these drugs on the CNS of children born to HIV positive mothers undergone to antiretroviral treatment.

scholarly journals Sulforaphane as an inducer of glutathione prevents oxidative stress-induced cell death in a dopaminergic-like neuroblastoma cell line

2009 ◽  
Vol 111 (5) ◽  
pp. 1161-1171 ◽  
Author(s):  
Andrea Tarozzi ◽  
Fabiana Morroni ◽  
Adriana Merlicco ◽  
Silvana Hrelia ◽  
Cristina Angeloni ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Cell Line ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line
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