Entrapment of porous cross-linked enzyme aggregates of maltogenic amylase from Bacillus lehensis G1 into calcium alginate for maltooligosaccharides synthesis

2020 ◽  
Vol 150 ◽  
pp. 80-89 ◽  
Author(s):  
Noor Namirah Nawawi ◽  
Zanariah Hashim ◽  
Roshanida A. Rahman ◽  
Abdul Munir Abdul Murad ◽  
Farah Diba Abu Bakar ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Maltogenic Amylase

A Dual Sensor for Biogenic Amines and Oxygen Based on Genipin Immobilized in Edible Calcium Alginate Gel Beads

2020 ◽  
Author(s):  
Ian Mallov ◽  
Fiona Jeeva ◽  
Chris Caputo
Keyword(s):  
Biogenic Amines ◽  
Calcium Alginate ◽  
Alginate Beads ◽  
Colorimetric Sensing ◽  
Naturally Occurring ◽  
Gel Beads ◽  
Dual Sensor ◽  
Calcium Alginate Gel ◽  
Toxic Food

Food is often wasted due to real or perceived concerns about preservation and shelf life. Thus, precise, accurate and consumer-friendly methods of indicating whether food is safe for consumers are drawing great interest. The colorimetric sensing of biogenic amines released as food degrades is a potential way of determining the quality of the food. Herein, we report the use of genipin, a naturally occurring iridoid, as a dual colorimetric sensor for both oxygen and biogenic amines. Immobilization of genipin in edible calcium alginate beads demonstrates that it is a capable sensor for amine vapors and can be immobilized in a non-toxic, food-friendly matrix.

Nitrification and Autotrophic Denitrification in Calcium Alginate Beads

1987 ◽  
Vol 19 (1-2) ◽  
pp. 175-182 ◽  
Author(s):  
Z. Lewandowski ◽  
R. Bakke ◽  
W. G. Characklis
Keyword(s):  
Calcium Carbonate ◽  
Calcium Ions ◽  
Continuous Flow ◽  
Calcium Alginate ◽  
Microbial Respiration ◽  
Flow System ◽  
Alginate Beads ◽  
Alginate Gel ◽  
Gel Beads ◽  
Calcium Alginate Gel

Immobilization of nitrifiers and autotrophic denitrifiers (Thiobacillus denitrificans) within calcium alginate gel was demonstrated. Calcium carbonate reagent was immobilized along with bacteria as the stabilizing agent. Protons released as a result of microbial respiration reacted with calcium carbonate producing calcium ions which internally stabilized the calcium alginate gel. The microbially active gel beads were mechanically stable and active for three months in a continuous flow system without addition of calcium.

Effect of co-encapsulation using a calcium alginate matrix and fructooligosaccharides with gelatin coating on the survival of Lactobacillus paracasei cells

2021 ◽  
Author(s):  
Rosetânia Correia Neves da Conceição ◽  
Rayssa Dias Batista ◽  
Fernanda Munhoz dos Anjos Leal Zimmer ◽  
Ianna Kelly Martins Trindade ◽  
Alex Fernando de Almeida ◽  
...  
Keyword(s):  
Calcium Alginate ◽  
Lactobacillus Paracasei ◽  
Gelatin Coating ◽  
Alginate Matrix

Evaluation of calcium alginate bead formation kinetics: an integrated analysis through light microscopy, rheology and microstructural SAXS

2021 ◽  
pp. 118293
Author(s):  
Andrés Posbeyikian ◽  
Esteban Tubert ◽  
Alejandro Bacigalupe ◽  
Mariano Martin Escobar ◽  
Patricio Román Santagapita ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Calcium Alginate ◽  
Alginate Bead ◽  
Integrated Analysis ◽  
Calcium Alginate Bead ◽  
Bead Formation ◽  
Formation Kinetics

Characteristics and mechanism of Cu(II) adsorption on prepared calcium alginate/carboxymethyl cellulose@MnFe2O4

2021 ◽  
Author(s):  
Changjiang Yu ◽  
Han Li ◽  
Haoyue Ma ◽  
Limei Zhang ◽  
Yu Li ◽  
...  
Keyword(s):  
Carboxymethyl Cellulose ◽  
Calcium Alginate

scholarly journals Green Production of Cladribine by Using Immobilized 2′-Deoxyribosyltransferase from Lactobacillus delbrueckii Stabilized through a Double Covalent/Entrapment Technology

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 657
Author(s):  
Cintia Wanda Rivero ◽  
Natalia Soledad García ◽  
Jesús Fernández-Lucas ◽  
Lorena Betancor ◽  
Gustavo Pablo Romanelli ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Enzymatic Synthesis ◽  
Calcium Alginate ◽  
Covalent Immobilization ◽  
Chemical Processes ◽  
Lactobacillus Delbrueckii ◽  
Proof Of Concept ◽  
Enzymatic Production ◽  
Green Production ◽  
Efficient Alternative

Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60 °C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale.

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