Effect of co-encapsulation using a calcium alginate matrix and fructooligosaccharides with gelatin coating on the survival of Lactobacillus paracasei cells

Author(s):  
Rosetânia Correia Neves da Conceição ◽  
Rayssa Dias Batista ◽  
Fernanda Munhoz dos Anjos Leal Zimmer ◽  
Ianna Kelly Martins Trindade ◽  
Alex Fernando de Almeida ◽  
...  
2005 ◽  
Vol 16 (1) ◽  
pp. 9-13 ◽  
Author(s):  
R. HURTEAUX ◽  
H. Benhayoune ◽  
F. Edwards-Levy ◽  
S. Bouthors ◽  
G. Balossier ◽  
...  

Biologia ◽  
2010 ◽  
Vol 65 (3) ◽  
Author(s):  
Shuvashish Behera ◽  
Rama Mohanty ◽  
Ramesh Ray

AbstractMahula (Madhuca latifolia L.) is a deciduous tree commonly found in the tropical rain forests of Asian and Australian continent. Corolla, the edible part of its flowers, is rich in fermentable sugar (37 ± 0.23%; on dry weight basis). Batch fermentation of mahula flowers was carried out using Zymomonas mobilis MTCC 92 free cells and cells immobilized in calcium alginate matrix. The ethanol productions were 122.9 ± 0.972 and 134.6 ± 0.104 g/kg flowers on dry weight basis using free and immobilized cells, respectively, after 96 h of fermentation, which showed that cells entrapped in calcium alginate matrix yielded 8.7% more ethanol than free cells. Further, the immobilized cells were physiologically active up to three more cycles of fermentation producing 132.7 ± 0.095, 130.5 ± 0.09 and 128.7 ± 0.056 g ethanol per kg flower in first, second and third cycle, respectively.


Author(s):  
Concetta Di Natale ◽  
Elena Lagreca ◽  
Valeria Panzetta ◽  
Marianna Gallo ◽  
Francesca Passannanti ◽  
...  

The intestinal microbiota is a real ecosystem composed of several bacterial species and a very huge amount of strains that through their metabolic activities play a crucial role in the development and performance of the immune system and other functions. Microbiota modulation by probiotics establishes a new era into the pharmaceutical and healthcare market. Probiotics play, in fact, an important role in helping and sustaining human health, but in order to produce benefits, their viability must be preserved throughout the production process up to consumption, and in addition, their bioactivity required to be safeguarded while passing through the gastrointestinal tract. In this frame, encouraging results come from encapsulation strategies that have proven to be very promising in protecting bacteria and their viability. However, specific effort has to be dedicated to the design optimization of the encapsulation process and, in particular, to the processing parameters that affect capsules microstructure. Herein, focusing on calcium alginate microspheres, after a preliminary selection of their processing conditions based on size distribution, we implemented a micro-rheological analysis, by using the multiple-particle tracking technique, to correlate the inner microstructure to the selected process conditions and to the viability of the Lactobacillus paracasei CBA L74. It was assessed that the explored levels of cross-linking, although changing the microorganism constriction, did not affect its viability. The obtained results confirm how this technology is a promising and a valid strategy to protect the microorganism viability and ensure its stability during the production process.


2008 ◽  
Vol 28 (2) ◽  
pp. 253-257 ◽  
Author(s):  
M.A. Morales ◽  
P.V. Finotelli ◽  
J.A.H. Coaquira ◽  
M.H.M. Rocha-Leão ◽  
C. Diaz-Aguila ◽  
...  

Polymers ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 170
Author(s):  
Lăcrămioara Rusu ◽  
Cristina-Gabriela Grigoraș ◽  
Andrei-Ionuț Simion ◽  
Elena-Mirela Suceveanu ◽  
Alexandra-Cristina Blaga ◽  
...  

Pharmaceuticals are recognized as emerging water microcontaminants that have been reported in several aquatic environments worldwide; therefore, the elimination of these pollutants is a global challenge. This study aimed to develop a biosorbent based on Saccharomyces pastorianus residual biomass encapsulated in a calcium alginate matrix and to evaluate its biosorption performance to remove Ethacridine Lactate (EL) from aqueous solutions. Firstly, the synthesis and characterization of biosorbent has been carried out. Then, the impact of main parameters on biosorption process were investigated by batch experiments. Finally, the kinetics behavior and equilibrium isotherms were evaluated. The resulted beads have an irregular and elongated shape with about 1.89 mm ± 0.13 mm in size with a homogeneous structure. The best removal efficiency for EL of over 85% was obtained at acidic pH 2 and 25 °C for 50 mg/L initial concentration and 2 g/L biosorbent dose. The pseudo-second-order and intraparticle diffusion kinetics describe the biosorption process. The maximum calculated biosorption capacity was 21.39 mg/g similar to that recorded experimentally. The equilibrium biosorption data were a good fit for Freundlich and Dubinin–Radushkevich isotherms. Our findings reveal that the low cost and eco-friendly obtained biosorbent can be easily synthesized and suitable to remove Ethacridine Lactate from water matrices.


2000 ◽  
Vol 42 (5-6) ◽  
pp. 85-90 ◽  
Author(s):  
Y.P. Ting ◽  
G. Sun

In this work, we compared the performance of a new method of cell immobilization in a novel matrix for biosorption. Yeast cells were entrapped in a polyvinyl alcohol (PVA) matrix, based on an adapted iterative freeze-thaw-freeze process. Spherical and uniform beads were produced, and SEM micrographs confirmed that the cells were uniformly dispersed within the PVA matrix. Further experiments revealed that the use of PVA as the immobilization matrix conferred better mechanical and chemical properties than the commonly used calcium alginate matrix. Experiments also showed that the PVA matrix gave rise to a lower mass transfer resistance than the alginate matrix. Finally, it was established that PVA-yeast biosorbent beads could be regenerated using dilute hydrochloric acid (10mM) and reused for at least five biosorption cycles with virtually no decrease in its bisorption capacity. Different metal/biosorbent in PVA-immobilized systems are currently being investigated.


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