The discovery and characterization of a novel chitinase with dual catalytic domains from a Qinghai-Tibetan Plateau wetland soil metagenome

2021 ◽  
Author(s):  
Yumei Dai ◽  
Feng Yang ◽  
Xiao Liu ◽  
Hongling Wang
Keyword(s):  
Tibetan Plateau ◽  
Wetland Soil ◽  
Catalytic Domains ◽  
Plateau Wetland ◽  
Soil Metagenome

scholarly journals Cloning and characterization of a thermostable and alkaline fibrinolytic enzyme from a soil metagenome

2013 ◽  
Vol 12 (45) ◽  
pp. 6389-6399 ◽  
Author(s):  
Leedagger; Sun-Yi ◽  
Yudagger; Sun-Nyoung ◽  
Choi Hak-Jong ◽  
Kim Kwang-Youn ◽  
Kim Sang-Hun ◽  
...  
Keyword(s):  
Fibrinolytic Enzyme ◽  
Soil Metagenome

scholarly journals Biochemical characterization of a GH70 protein from Lactobacillus kunkeei DSM 12361 with two catalytic domains involving branching sucrase activity

2018 ◽  
Vol 102 (18) ◽  
pp. 7935-7950 ◽  
Author(s):  
Xiangfeng Meng ◽  
Joana Gangoiti ◽  
Xiaofei Wang ◽  
Pieter Grijpstra ◽  
Sander S. van Leeuwen ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Sucrase Activity ◽  
Catalytic Domains ◽  
Lactobacillus Kunkeei

Isolation and characterization of two lytic cold-active bacteriophages infecting Pseudomonas fluorescens from the Napahai plateau wetland

2018 ◽  
Vol 64 (3) ◽  
pp. 183-190 ◽  
Author(s):  
Yingying Xiang ◽  
Shuang Wang ◽  
Jiankai Li ◽  
Yunlin Wei ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Flood Control ◽  
Long Tail ◽  
Ph Values ◽  
Plateau Wetland ◽  
Isolation And Characterization ◽  
Adaptation Mechanisms ◽  
The Earth ◽  
Cold Active

As the “kidneys of the Earth”, wetlands play important roles as biodiversity reservoirs, in water purification, and in flood control. In this study, 2 lytic cold-active bacteriophages, named VW-6S and VW-6B, infecting Pseudomonas fluorescens W-6 cells from the Napahai plateau wetland in China were isolated and characterized. Electron microscopy showed that both VW-6S and VW-6B had an icosahedral head (66.7 and 61.1 nm, respectively) and a long tail (8.3 nm width × 233.3 nm length and 11.1 nm width × 166.7 nm length, respectively). The bacteriophages VW-6S and VW-6B were classified as Siphoviridae and had an approximate genome size of 30–40 kb. The latent and burst periods of VW-6S were 60 and 30 min, whereas those of VW-6B were 30 and 30 min, respectively. The optimal pH values for the bacteriophages VW-6S and VW-6B were 8.0 and 10.0, respectively, and their activity decreased rapidly at temperatures higher than 60 °C. These cold-active bacteriophages provide good materials for further study of cold-adaptation mechanisms and interaction with the host P. fluorescens.

Sign in / Sign up

Export Citation Format

Share Document