Vertical distribution patterns and drivers of soil bacterial communities across the continuous permafrost region of northeastern China

Background Soil microorganisms in the thawing permafrost play key roles in the maintenance of ecosystem function and regulation of biogeochemical cycles. However, our knowledge of patterns and drivers of permafrost microbial communities is limited in northeastern China. Therefore, we investigated the community structure of soil bacteria in the active, transition and permafrost layers based on 90 soil samples collected from 10 sites across the continuous permafrost region using high-throughput Illumina sequencing. Results Proteobacteria (31.59%), Acidobacteria (18.63%), Bacteroidetes (9.74%), Chloroflexi (7.01%) and Actinobacteria (6.92%) were the predominant phyla of the bacterial community in all soil layers; however, the relative abundances of the dominant bacterial taxa varied with soil depth. The bacterial community alpha-diversity based on the Shannon index and the phylogenetic diversity index both decreased significantly with depth across the transition from active layer to permafrost layer. Nonmetric multidimensional scaling analysis and permutation multivariate analysis of variance revealed that microbial community structures were significantly different among layers. Redundancy analysis and Spearman’s correlation analysis showed that soil properties differed between layers such as soil nutrient content, temperature and moisture mainly drove the differentiation of bacterial communities. Conclusions Our results revealed significant differences in bacterial composition and diversity among soil layers. Our findings suggest that the heterogeneous environmental conditions between the three soil horizons had strong influences on microbial niche differentiation and further explained the variability of soil bacterial community structures. This effort to profile the vertical distribution of bacterial communities may enable better evaluations of changes in microbial dynamics in response to permafrost thaw, which would be beneficial to ecological conservation of permafrost ecosystems.

Vegetation Mapping in the Permafrost Region: A Case Study on the Central Qinghai-Tibet Plateau

An accurate and detailed vegetation map is of crucial significance for understanding the spatial heterogeneity of subsurfaces, which can help to characterize the thermal state of permafrost. The absence of an alpine swamp meadow (ASM) type, or an insufficient resolution (usually km-level) to capture the spatial distribution of the ASM, greatly limits the availability of existing vegetation maps in permafrost modeling of the Qinghai-Tibet Plateau (QTP). This study generated a map of the vegetation type at a spatial resolution of 30 m on the central QTP. The random forest (RF) classification approach was employed to map the vegetation based on 319 ground-truth samples, combined with a set of input variables derived from the visible, infrared, and thermal Landsat-8 images. Validation using a train-test split (i.e., 70% of the samples were randomly selected to train the RF model, while the remaining 30% were used for validation and a total of 1000 runs) showed that the average overall accuracy and Kappa coefficient of the RF approach were 0.78 (0.68–0.85) and 0.69 (0.64–0.74), respectively. The confusion matrix showed that the overall accuracy and Kappa coefficient of the predicted vegetation map reached 0.848 (0.844–0.852) and 0.790 (0.785–0.796), respectively. The user accuracies for the ASM, alpine meadow, alpine steppe, and alpine desert were 95.0%, 83.3%, 82.4%, and 86.7%, respectively. The most important variables for vegetation type prediction were two vegetation indices, i.e., NDVI and EVI. The surface reflectance of visible and shortwave infrared bands showed a secondary contribution, and the brightness temperature and the surface temperature of the thermal infrared bands showed little contribution. The dominant vegetation in the study area is alpine steppe and alpine desert. The results of this study can provide an accurate and detailed vegetation map, especially for the distribution of the ASM, which can help to improve further permafrost studies.

Organic Carbon Mineralization and Bacterial Community of Active Layer Soils Response to Short-Term Warming in the Great Hing’an Mountains of Northeast China

As a buffer layer for the energy and water exchange between atmosphere and permafrost, the active layer is sensitive to climate warming. Changes in the thermal state in active layer can alter soil organic carbon (SOC) dynamics. It is critical to identify the response of soil microbial communities to warming to better predict the regional carbon cycle under the background of global warming. Here, the active layer soils collected from a wetland-forest ecotone in the continuous permafrost region of Northeastern China were incubated at 5 and 15°C for 45 days. High-throughput sequencing of the 16S rRNA gene was used to examine the response of bacterial community structure to experimental warming. A total of 4148 OTUs were identified, which followed the order 15°C > 5°C > pre-incubated. Incubation temperature, soil layer and their interaction have significant effects on bacterial alpha diversity (Chao index). Bacterial communities under different temperature were clearly distinguished. Chloroflexi, Actinobacteria, Proteobacteria, and Acidobacteria accounted for more than 80% of the community abundance at the phylum level. Warming decreased the relative abundance of Chloroflexi and Acidobacteria, while Actinobacteria and Proteobacteria exhibited increasing trend. At family level, the abundance of norank_o__norank_c__AD3 and Ktedonobacteraceae decreased significantly with the increase of temperature, while Micrococcaccac increased. In addition, the amount of SOC mineralization were positively correlated with the relative abundances of most bacterial phyla and SOC content. SOC content was positively correlated with the relative abundance of most bacterial phyla. Results indicate that the SOC content was the primary explanatory variable and driver of microbial regulation for SOC mineralization. Our results provide a new perspective for understanding the microbial mechanisms that accelerates SOC decomposition under warming conditions in the forest-wetland ecotone of permafrost region.

Stability of the Foundation of Buried Energy Pipeline in Permafrost Region

During operation, a buried pipeline is threatened by a variety of geological hazards, particularly in permafrost regions, where freezing-thawing disasters have a significant influence on the integrity and safety of the buried pipelines. The topographical environmental conditions along the pipeline, as well as the influence of frost heave and thaw settlement on the pipeline’s foundation soil, must be considered in the design and construction stage. Theoretical analysis, numerical modeling, field testing, and mitigation measures on vital energy pipelines in permafrost have been widely documented, but no attempt has been made to review the freezing-thawing disasters, current research methodologies, and mitigation strategies. This article reviews the formation mechanisms and mitigation measures for frost hazards (e.g., differential frost heave, thaw settlement, slope instability, frost mounds, icing, river ice scouring, and pipeline floating) along buried pipelines in permafrost regions and summarizes and prospects the major progress in the research on mechanisms, analysis methods, model test, and field monitoring based on publications of studies of key energy pipelines in permafrost regions. This review will provide scholars with a basic understanding of the challenging freezing-thawing hazards encountered by energy pipelines in permafrost regions, as well as research on the stability and mitigation of pipeline foundation soils plagued by freezing-thawing hazards in permafrost regions under a warming climate and degrading permafrost environment.

A high-resolution inland surface water body dataset for the tundra and boreal forests of North America

Inland surface waters are abundant in the tundra and boreal forests in North America, essential to environments and human societies but vulnerable to climate changes. These high-latitude water bodies differ greatly in their morphological and topological characteristics related to the formation, type, and vulnerability. In this paper we present an inland surface water body inventory (SWBI) dataset for the tundra and boreal forests of North America. Nearly 6.7 million water bodies were identified, with approximately 6 million (~90 %) of them smaller than 0.1 km2. The dataset provides geometry coverage and morphological attributes for every water body. During this study we developed an automated approach for detecting surface water extent and identifying water bodies in the 10 m resolution Sentinel-2 multispectral satellite data to enhance the capability for delineating small water bodies and their morphological attributes. The approach was applied to the Sentinel-2 data acquired in 2019 to produce the water body dataset for the entire tundra and boreal forests in North America, providing a more complete representation of the region than existing regional datasets, e.g., Permafrost Region Pond and Lake (PeRL). Total accuracy of the detected water extent by SWBI dataset was 96.36 % by comparing to interpreted data for locations randomly sampled across the region. Compared to the 30 m or coarser resolution water datasets, e.g., JRC GSW yearly water history, HydroLakes, and Global Lakes and Wetlands Database (GLWD), the SWBI provided an improved ability on delineating water bodies, and reported higher accuracies in the size, number, and perimeter attributes of water body by comparing to PeRL and interpreted regional dataset. This dataset is available on the National Tibetan Plateau/Third Pole Environment Data Center (TPDC, http://data.tpdc.ac.cn): DOI: 10.11888/Hydro.tpdc.271021 (Feng et al., 2020).