A novel linear epitope at the C-terminal region of the classical swine fever virus E2 protein elicits neutralizing activity

2021 ◽  
Author(s):  
Qianru Xu ◽  
Junqing Guo ◽  
Fanshu Ma ◽  
Linke Liu ◽  
Yanan Wang ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Terminal Region ◽  
Fever Virus ◽  
Linear Epitope ◽  
E2 Protein ◽  
Neutralizing Activity

scholarly journals Development of whole-porcine monoclonal antibodies with potent neutralization activity against classical swine fever virus (CSFV) from single B cells

2018 ◽  
Author(s):  
Haisi Dong ◽  
Dongmei Lv ◽  
Ang Su ◽  
Lerong Ma ◽  
Jianwei Dong ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
B Cells ◽  
Virus Infection ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Economic Losses ◽  
E2 Protein ◽  
Neutralizing Activity

AbstractClassical swine fever (CSF) is a highly contagious swine disease found worldwide that has caused devastating economic losses. However, there are few efficacious mAbs against the CSF virus (CSFV) that can be used for treatment because most mAbs against CSFV are derived from mouse hybridoma cells and these murine mAbs have disadvantages of inefficient effector functions elicitations and high immunogenicity in vivo. Accordingly, we characterized whole-porcine anti-CSFV neutralizing mAbs (NAbs) isolated directly from single B cells sorted from a CSFV-vaccinated pig using the fluoresceinated conserved linear neutralizing epitope of the CSFV E2 protein and fluorophore conjugated goat anti-pig IgG. Immunoglobulin (Ig) genes were isolated via nested PCR, and two porcine mAbs termed HK24 and HK44 were produced. We determined that these mAbs can bind to E2 protein and recognize sites within this major antigenic epitope. In addition, we found that mAbs HK24 and HK44 exhibit potent neutralizing activity against CSFV, and they can protect PK-15 cells from infections in vitro with potent IC50 values of 9.3 μg/ml and 0.62 μg/ml, respectively. Notably, we demonstrated that these two mAbs can be used as novel reagents for detecting virus infection. These data suggest that our results not only provide a method for efficiently obtaining mAbs against CSFV but also offer promising mAb candidates for development of antibody-based diagnostic and antiviral agents.ImportanceNeutralizing monoclonal antibodies (NAbs) can prevent and may slow the spread of virus infection. The discovery of NAbs that recognize classical swine fever virus (CSFV) necessitates new technologies because the NAbs produced by immunization and hybridoma technology could not be transferred to in vivo research. Multiple full-length human therapeutic antibodies have been produced via single-cell polymerase chain reactions but whole-porcine NAbs for CSFV have not been generated. In this study, two whole-porcine mAbs, named HK24 and HK44, were isolated from epitope-specific single B cells. We demonstrate that these two mAbs have potent neutralizing activity against CSFV and can protect cells against viral infection. Therefore, they may facilitate the development of vaccines or antiviral drugs that offer the advantages of stability and low immunogenicity.

Identification of conformational epitopes and antigen-specific residues at the D/A domains and the extramembrane C-terminal region of E2 glycoprotein of classical swine fever virus

2012 ◽  
Vol 168 (1-2) ◽  
pp. 56-63 ◽  
Author(s):  
Chia-Yi Chang ◽  
Chin-Cheng Huang ◽  
Ming-Chung Deng ◽  
Yeou-Liang Huang ◽  
Yu-Ju Lin ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Terminal Region ◽  
Fever Virus ◽  
E2 Glycoprotein ◽  
Conformational Epitopes ◽  
A Domains

scholarly journals Immunogenicity in Swine of Orally Administered Recombinant Lactobacillus plantarum Expressing Classical Swine Fever Virus E2 Protein in Conjunction with Thymosin α-1 as an Adjuvant

2015 ◽  
Vol 81 (11) ◽  
pp. 3745-3752 ◽  
Author(s):  
Yi-Gang Xu ◽  
Xue-Ting Guan ◽  
Zhong-Mei Liu ◽  
Chang-Yong Tian ◽  
Li-Chun Cui
Keyword(s):  
Immune Responses ◽  
Lactobacillus Plantarum ◽  
Classical Swine Fever Virus ◽  
Oral Vaccine ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Economic Losses ◽  
E2 Protein ◽  
Content Type ◽  
Cellular Immune

ABSTRACTClassical swine fever, caused by classical swine fever virus (CSFV), is a highly contagious disease that results in enormous economic losses in pig industries. The E2 protein is one of the main structural proteins of CSFV and is capable of inducing CSFV-neutralizing antibodies and cytotoxic T lymphocyte (CTL) activitiesin vivo. Thymosin α-1 (Tα1), an immune-modifier peptide, plays a very important role in the cellular immune response. In this study, genetically engineeredLactobacillus plantarumbacteria expressing CSFV E2 protein alone (L. plantarum/pYG-E2) and in combination with Tα1 (L. plantarum/pYG-E2-Tα1) were developed, and the immunogenicity of each as an oral vaccine to induce protective immunity against CSFV in pigs was evaluated. The results showed that recombinantL. plantarum/pYG-E2 andL. plantarum/pYG-E2-Tα1 were both able to effectively induce protective immune responses in pigs against CSFV infection by eliciting immunoglobulin A (IgA)-based mucosal, immunoglobulin G (IgG)-based humoral, and CTL-based cellular immune responses via oral vaccination. Significant differences (P< 0.05) in the levels of immune responses were observed betweenL. plantarum/pYG-E2-Tα1 andL. plantarum/pYG-E2, suggesting a better immunogenicity ofL. plantarum/pYG-E2-Tα1 as a result of the Tα1 molecular adjuvant that can enhance immune responsiveness and augment specific lymphocyte functions. Our data suggest that the recombinantLactobacillusmicroecological agent expressing CSFV E2 protein combined with Tα1 as an adjuvant provides a promising strategy for vaccine development against CSFV.

An improved indirect ELISA for specific detection of antibodies against classical swine fever virus based on structurally designed E2 protein expressed in suspension mammalian cells

2018 ◽  
Vol 163 (7) ◽  
pp. 1831-1839
Author(s):  
Shengwei Ji ◽  
Yuzi Luo ◽  
Tengteng Zhang ◽  
Lina Shao ◽  
Xing-Yu Meng ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Mammalian Cells ◽  
Indirect Elisa ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Specific Detection ◽  
E2 Protein

Hypervariable antigenic region 1 of classical swine fever virus E2 protein impacts antibody neutralization

Vaccine ◽  
2016 ◽  
Vol 34 (33) ◽  
pp. 3723-3730 ◽  
Author(s):  
Xun Liao ◽  
Zuohuan Wang ◽  
Tong Cao ◽  
Chao Tong ◽  
Shichao Geng ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Antibody Neutralization ◽  
E2 Protein ◽  
Antigenic Region

Induction of immune responses in mice and pigs by oral administration of classical swine fever virus E2 protein expressed in rice calli

2014 ◽  
Vol 159 (12) ◽  
pp. 3219-3230 ◽  
Author(s):  
Myunghwan Jung ◽  
Yun Ji Shin ◽  
Ju Kim ◽  
Seung-Bin Cha ◽  
Won-Jung Lee ◽  
...  
Keyword(s):  
Immune Responses ◽  
Oral Administration ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Protein ◽  
Rice Calli

Immunogenicity of a lettuce-derived vaccine candidate expressing the E2 protein against classical swine fever virus

2013 ◽  
Vol 113 (3) ◽  
pp. 483-490 ◽  
Author(s):  
Jinn-Chin Yiu ◽  
Cheng-Wei Liu ◽  
Ruei-Yuan Su ◽  
Wan-Jun Lai ◽  
Menq-Jiau Tseng ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Vaccine Candidate ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Protein

Expression and purification of classical swine fever virus E2 protein from Sf9 cells using a modified vector

2017 ◽  
Vol 39 (12) ◽  
pp. 1821-1825 ◽  
Author(s):  
Ling Yang ◽  
Xingmeng Lu ◽  
Weihuan Fang
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Sf9 Cells ◽  
Expression And Purification ◽  
E2 Protein

scholarly journals Mutations in the carboxyl terminal region of E2 glycoprotein of classical swine fever virus are responsible for viral attenuation in swine

Virology ◽  
2007 ◽  
Vol 364 (2) ◽  
pp. 371-382 ◽  
Author(s):  
G.R. Risatti ◽  
L.G. Holinka ◽  
I. Fernandez Sainz ◽  
C. Carrillo ◽  
G.F. Kutish ◽  
...  
Keyword(s):  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Terminal Region ◽  
Fever Virus ◽  
E2 Glycoprotein ◽  
Carboxyl Terminal ◽  
Viral Attenuation
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