A pulsed field gel electrophoresis (PFGE) study that suggests a major world-wide clone of Salmonella enterica serovar Enteritidis

2007 ◽  
Vol 116 (3) ◽  
pp. 305-312 ◽  
Author(s):  
Jen-Chieh Pang ◽  
Tsai-Hsin Chiu ◽  
Reiner Helmuth ◽  
Andreas Schroeter ◽  
Beatriz Guerra ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
World Wide ◽  
Pulsed Field Gel Electrophoresis ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Pulsed Field

scholarly journals Genome Sequences of Two Strains of Salmonella enterica Serovar Enteritidis Isolated from Shell Eggs

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Matthew R. Moreau ◽  
Dona Saumya S. Wijetunge ◽  
Eranda Mangala K. Kurundu Hewage ◽  
Bhushan M. Jayarao ◽  
Subhashinie Kariyawasam
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Complete Genome ◽  
Pulsed Field Gel Electrophoresis ◽  
Genome Sequences ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Pulsed Field ◽  
Shell Eggs

This report presents the complete genome sequences of two Salmonella enterica serovar Enteritidis strains bearing the pulsed-field gel electrophoresis profile JEGX01.0004, which were isolated from the internal contents of eggs.

scholarly journals Suitability of PCR Fingerprinting, Infrequent-Restriction-Site PCR, and Pulsed-Field Gel Electrophoresis, Combined with Computerized Gel Analysis, in Library Typing of Salmonella enterica Serovar Enteritidis

2000 ◽  
Vol 66 (12) ◽  
pp. 5273-5281 ◽  
Author(s):  
Javier Garaizar ◽  
Nuria L�pez-Molina ◽  
Idoia Laconcha ◽  
Dorte Lau Baggesen ◽  
Aitor Rementeria ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Restriction Site ◽  
Pulsed Field Gel Electrophoresis ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Pulsed Field ◽  
Pcr Fingerprinting ◽  
Phage Types ◽  
High Discrimination ◽  
Dna Profiles

ABSTRACT Strains of Salmonella enterica (n = 212) of different serovars and phage types were used to establish a library typing computerized system for serovar Enteritidis on the basis of PCR fingerprinting, infrequent-restriction-site PCR (IRS-PCR), or pulsed-field gel electrophoresis (PFGE). The rate of PCR fingerprinting interassay and intercenter reproducibility was low and was only increased when DNA samples were extracted at the same time and amplified with the same reaction mixtures. Reproducibility of IRS-PCR technique reached 100%, but discrimination was low (D= 0.52). The PFGE procedure showed an intercenter reproducibility value of 93.3%. The high reproducibility of PFGE combined with the previously determined high discrimination directed its use for library typing. The use of PFGE with enzymes XbaI,BlnI, and SpeI for library typing of serovar Enteritidis was assessed with GelCompar 4.0 software. Three computer libraries of PFGE DNA profiles were constructed, and their ability to recognize new DNA profiles was analyzed. The results obtained pointed out that the combination of PFGE with computerized analysis could be suitable in long-term epidemiological comparison and surveillance ofSalmonella serovar Enteritidis, specially if the prevalence of genetic events that could be responsible for changes in PFGE profiles in this serovar was low.

scholarly journals Molecular Analyses of Salmonellaenterica Isolates from Fish Feed Factories and Fish Feed Ingredients

2003 ◽  
Vol 69 (2) ◽  
pp. 1075-1081 ◽  
Author(s):  
Live L. Nesse ◽  
Kerstin Nordby ◽  
Even Heir ◽  
Bjarne Bergsjoe ◽  
Traute Vardund ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Profile Analysis ◽  
Plasmid Profile ◽  
Pulsed Field Gel Electrophoresis ◽  
Fish Feed ◽  
Molecular Analyses ◽  
Feed Ingredients ◽  
Pulsed Field ◽  
Salmonella Serovars

ABSTRACT Isolates of the most commonly observed salmonella serovars in Norwegian fish feed factories from 1998 to 2000 (Salmonella enterica serovar Agona, S. enterica serovar Montevideo, S. enterica serovar Senftenberg, and S. enterica serovar Kentucky) were studied by pulsed-field gel electrophoresis (PFGE) and plasmid profile analysis and compared to isolates of the same serovars from fish feed ingredients, humans, and other sources (a total of 112 isolates). Within each serovar, a variety of distinct PFGE types (with similarity levels less than 90%) were observed in the feed ingredients and other sources, while only two distinct types of each serovar were identified in the factories. The combined results of PFGE and plasmid analyses showed that each factory harbored only a few S. enterica clones. Some of these clones persisted for at least 3 years in the factories, indicating that there was long-lasting contamination probably due to inadequate decontamination procedures.

scholarly journals Outbreak of Salmonella enterica serotype Muenster infections associated with goat's cheese, France, March 2008

2009 ◽  
Vol 14 (31) ◽  
Author(s):  
D van Cauteren ◽  
N Jourdan-da Silva ◽  
F X Weill ◽  
L King ◽  
A Brisabois ◽  
...  
Keyword(s):  
Food Consumption ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Pulsed Field Gel Electrophoresis ◽  
Gastrointestinal Illness ◽  
Pulsed Field

Salmonella enterica serotype Muenster (hereafter referred to as S. Muenster) is rare in France and in Europe. In France, a nationwide outbreak of gastrointestinal illness due to S. Muenster occurred during March and April 2008. Twenty-five laboratory-confirmed cases of S. Muenster were documented by telephone using a trawling questionnaire. Four patients were admitted to hospital and no death was recorded. Among the 21 interviewed cases, 16 reported consumption of goat's cheese in the days prior to symptoms. The investigation incriminated goat's cheese from producer X as being the most likely source of the outbreak. S. Muenster was isolated from both cases and the incriminated goat's cheese. The pulsed-field gel electrophoresis profiles of the food isolates of producer X and the isolates from cases were indistinguishable. Following the withdrawal of the contaminated batch of cheese, the number of cases decreased to its usual level. To our knowledge, this is the first published outbreak of S. Muenster associated with food consumption in Europe.

scholarly journals Nontyphoidal Salmonellae in United Kingdom Badgers: Prevalence and Spatial Distribution

2003 ◽  
Vol 69 (7) ◽  
pp. 4312-4315 ◽  
Author(s):  
J. Sian Wilson ◽  
Sarah M. Hazel ◽  
Nicola J. Williams ◽  
Amos Phiri ◽  
Nigel P. French ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
United Kingdom ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Social Groups ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

ABSTRACT Eighteen (72%) of 25 badger social groups were found to excrete Salmonella enterica serovar Ried, S. enterica serovar Binza, S. enterica serovar Agama, or S. enterica serovar Lomita. Each serovar was susceptible to a panel of antimicrobials. Based on results of pulsed-field gel electrophoresis, the S. enterica serovar Agama and S. enterica serovar Binza isolates were very similar, but two clones each of S. enterica serovar Lomita and S. enterica serovar Ried were found. Badgers excreting S. enterica serovar Agama were spatially clustered.

Sign in / Sign up

Export Citation Format

Share Document