scholarly journals Propidium monoazide combined with real-time quantitative PCR to quantify viable Alternaria spp. contamination in tomato products

2013 ◽  
Vol 165 (3) ◽  
pp. 214-220 ◽  
Author(s):  
Ana Crespo-Sempere ◽  
Núria Estiarte ◽  
Sonia Marín ◽  
Vicente Sanchis ◽  
Antonio J. Ramos
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Propidium Monoazide ◽  
Real Time Quantitative Pcr ◽  
Tomato Products

Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua

2011 ◽  
Vol 85 (2) ◽  
pp. 164-169 ◽  
Author(s):  
Trond Løvdal ◽  
Maria Befring Hovda ◽  
Benny Björkblom ◽  
Simon G. Møller
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Listeria Innocua ◽  
Propidium Monoazide ◽  
Real Time Quantitative Pcr

scholarly journals Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices

2006 ◽  
Vol 72 (12) ◽  
pp. 7894-7896 ◽  
Author(s):  
Silvia Bofill-Mas ◽  
Nestor Albinana-Gimenez ◽  
Pilar Clemente-Casares ◽  
Ayalkibet Hundesa ◽  
Jesus Rodriguez-Manzano ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
High Stability ◽  
Wastewater Sludge ◽  
Human Polyomavirus ◽  
Sewage Effluent ◽  
Viral Contamination ◽  
Human Adenoviruses ◽  
Urban Sewage ◽  
Real Time Quantitative Pcr

ABSTRACT Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

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