scholarly journals Identification of Mycobacterium tuberculosis complex in clinical specimens of HIV-infected patients at Instituto de Infectologia Emilio Ribas, São Paulo-Brazil

2016 ◽  
Vol 45 ◽  
pp. 393
Author(s):  
M.D. Eira ◽  
E. Boccardo ◽  
R.J. Costa Silva ◽  
U. Barbosa ◽  
I. Moreira ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Sao Paulo ◽  
São Paulo ◽  
Tuberculosis Complex ◽  
Clinical Specimens

scholarly journals Detection of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria by multiplex polymerase chain reactions

1999 ◽  
Vol 5 (1) ◽  
pp. 61-70
Author(s):  
A. S. Mustafa
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Mycobacterium Tuberculosis Complex ◽  
Clinical Isolates ◽  
Tuberculosis Complex ◽  
Clinical Specimens ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Polymerase Chain

The ability of two-band and three-band multiplex polymerase chain reactions to detect and differentiate Mycobacterium tuberculosis complex from non-tuberculous mycobacteria was evaluated. The polymerase chain reactions differentiated between M. tuberculosis and non-tuberculous mycobacteria when standard strains and clinical isolates of mycobacteria were tested. The sensitivity of the two-band and three-band techniques to detect M. tuberculosis in clinical specimens, compared with smear and/or culture, was 88% and 75% respectively. Although both techniques showed 100% specificity, the superior sensitivity of the two-band technique suggests that it could be more useful in the diagnosis of tuberculosis and in differentiating M. tuberculosis complex from non-tuberculous mycobacteria

Sign in / Sign up

Export Citation Format

Share Document