Inverse relationship between toxic shock syndrome toxin-1 antibodies and interferon-γ and interleukin-6 in peripheral blood mononuclear cells from patients with pediatric tonsillitis caused by Staphylococcus aureus

2017 ◽  
Vol 97 ◽  
pp. 211-217 ◽  
Author(s):  
Yinshuang Chen ◽  
Yanmei Huang ◽  
Bingshao Liang ◽  
Hui Dong ◽  
Shuwen Yao ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Interleukin 6 ◽  
Toxic Shock Syndrome ◽  
Inverse Relationship ◽  
Mononuclear Cells ◽  
Interferon Γ ◽  
Toxic Shock ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Toxic Shock Syndrome Toxin

scholarly journals Interaction of Staphylococcal Toxic Shock Syndrome Toxin-1 and Enterotoxin A on T Cell Proliferation and TNFα Secretion in Human Blood Mononuclear Cells

1999 ◽  
Vol 10 (6) ◽  
pp. 403-409
Author(s):  
Monica L De Boer ◽  
Winnie WS Kum ◽  
Anthony W Chow
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
Toxic Shock Syndrome ◽  
Mononuclear Cells ◽  
Human Pbmcs ◽  
Toxic Shock ◽  
Blood Mononuclear Cells ◽  
Toxic Shock Syndrome Toxin ◽  
Culture Supernatants

BACKGROUND: The majority of menstrual toxic shock syndrome (MTSS) cases are caused by a single clone ofStaphylococcus aureusthat produces both toxic shock syndrome toxin-1 (TSST-1) and staphylococcal enterotoxin A (SEA).OBJECTIVE: To determine whether the two superantigens interact to cause an enhancement of biological activity in human peripheral blood mononuclear cells (PBMCs).DESIGN: PBMCs from nine healthy donors were stimulated with TSST-1 or SEA, either alone or in combination at their minimum effective concentrations.SETTING: In vitro study.INTERVENTIONS: Human PBMCs were stimulated in vitro with TSST-1 (1 pg/mL), SEA (0.1 pg/mL) or combination for 20 to 72 h. Mitogenic response was determined by [3H]-thymidine incorporation. PBMC culture supernatants were assayed for the presence of tumour necrosis factor-alpha (TNFα), interleukin (IL)-1β and IL-6 by ELISA.MAIN RESULTS: The combination of TSST-1 and SEA induced significantly greater mitogenesis in human PBMCs compared with either toxin alone (P<0.05, paired Student’sttest, two-tailed). Similarly, the production of TNFα in culture supernatants was significantly greater in the combination of TSST-1 and SEA compared with either TSST-1 or SEA alone (P<0.05). In contrast, no enhancement in the levels IL-1 or IL-6 was observed.CONCLUSIONS: These data suggest that the co-production of TSST-1 and SEA byS aureusmay provide some biological advantage to the organism throughs an enhanced effect of these superantigens on T cell activation and TNF secretion.

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