scholarly journals The Specificity of Conditional Gene Targeting: A Case for Cre Reporters

Immunity ◽  
2019 ◽  
Vol 51 (4) ◽  
pp. 593-594 ◽  
Author(s):  
Boris Reizis
Keyword(s):  
Gene Targeting ◽  
Conditional Gene Targeting

scholarly journals Defining BMP functions in the hair follicle by conditional ablation of BMP receptor IA

2003 ◽  
Vol 163 (3) ◽  
pp. 609-623 ◽  
Author(s):  
Krzysztof Kobielak ◽  
H. Amalia Pasolli ◽  
Laura Alonso ◽  
Lisa Polak ◽  
Elaine Fuchs
Keyword(s):  
Hair Follicle ◽  
Progenitor Cells ◽  
Gene Targeting ◽  
Transcriptional Activation ◽  
Hair Shaft ◽  
Bmp Receptor ◽  
Root Sheath ◽  
Inner Root Sheath ◽  
Conditional Gene Targeting ◽  
Sequential Inhibition

Using conditional gene targeting in mice, we show that BMP receptor IA is essential for the differentiation of progenitor cells of the inner root sheath and hair shaft. Without BMPRIA activation, GATA-3 is down-regulated and its regulated control of IRS differentiation is compromised. In contrast, Lef1 is up-regulated, but its regulated control of hair differentiation is still blocked, and BMPRIA-null follicles fail to activate Lef1/β-catenin–regulated genes, including keratin genes. Wnt-mediated transcriptional activation can be restored by transfecting BMPRIA-null keratinocytes with a constitutively activated β-catenin. This places the block downstream from Lef1 expression but upstream from β-catenin stabilization. Because mice lacking the BMP inhibitor Noggin fail to express Lef1, our findings support a model, whereby a sequential inhibition and then activation of BMPRIA is necessary to define a band of hair progenitor cells, which possess enough Lef1 and stabilized β-catenin to activate the hair specific keratin genes and generate the hair shaft.

Conditional Gene Targeting in the Kidney

2005 ◽  
Vol 5 (5) ◽  
pp. 527-536 ◽  
Author(s):  
Alexander Gawlik ◽  
Susan Quaggin
Keyword(s):  
Gene Targeting ◽  
Conditional Gene Targeting

scholarly journals Optimized Vector for Conditional Gene Targeting in Mouse Embryonic Stem Cells

BioTechniques ◽  
2003 ◽  
Vol 34 (6) ◽  
pp. 1136-1140 ◽  
Author(s):  
Marcus Conrad ◽  
Markus Brielmeier ◽  
Wolfgang Wurst ◽  
Georg W. Bornkamm
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Gene Targeting ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Conditional Gene Targeting

Conditional gene targeting and its application in the skin

2000 ◽  
Vol 23 (3) ◽  
pp. 147-154 ◽  
Author(s):  
Junji Takeda ◽  
Shigetoshi Sano ◽  
Masahito Tarutani ◽  
Jiro Umeda ◽  
Gen Kondoh
Keyword(s):  
Gene Targeting ◽  
Conditional Gene Targeting

scholarly journals Microglia remodel the synaptic signaling required for context-dependent cognitive performance

2021 ◽  
Author(s):  
Renato Socodato ◽  
Tiago O. Almeida ◽  
Camila C. Portugal ◽  
Evelyn C. S. Santos ◽  
Joana Tedim-Moreira ◽  
...  
Keyword(s):  
Systems Biology ◽  
Gene Targeting ◽  
Cognitive Performance ◽  
Animal Behavior ◽  
Synaptic Activity ◽  
Rna Seq ◽  
Synaptic Remodeling ◽  
Conditional Gene Targeting ◽  
Context Dependent ◽  
The Brain

Microglia modulate synaptic activity, essential for context-dependent cognitive performance, allowing organism-level adaptations to different environmental scenarios. Yet, the microglial molecular drivers required for synaptic remodeling related to cognitive performance remain largely elusive. Here, combining conditional gene targeting, single-cell live imaging, RNA-seq, high-throughput proteomics, systems biology, and animal behavior, we mapped a molecular nexus between microglia and synapses that instruct cognitive performance. Specifically, we found that microglia use the RhoGTPase Rac1 as a relay switch to sense the brain microenvironment and drive synaptic remodeling required for experience-dependent sociability and learning related to memory. Targeting this microglial relay modifies context-dependent cognitive performance.

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