Optimization of alkali pretreatment for bioconversion of poplar (Populus deltoides) biomass into fermentable sugars using response surface methodology

2013 ◽  
Vol 44 ◽  
pp. 220-226 ◽  
Author(s):  
Rekha Rawat ◽  
B.K. Kumbhar ◽  
Lakshmi Tewari
2013 ◽  
Vol 59 (No. 12) ◽  
pp. 537-542 ◽  
Author(s):  
K. Jaisamut ◽  
L. Paulová ◽  
P. Patáková ◽  
M. Rychtera ◽  
K. Melzoch

Alkali pretreatment of wheat straw was optimized by response surface methodology to maximize yields of fermentable sugars in subsequent enzymatic hydrolysis and to remove maximum lignin in order to improve rheological attributes of the media. The effects of pretreatment conditions on biomass properties were studied using the Expert Designer software. Concentration of sodium hydroxide and temperature were the factors most affecting pretreatment efficiency. At the optimum (80°C, 39 min, 0.18 g NaOH and 0.06 g lime per g of raw biomass), 93.1 ± 1.0% conversion of cellulose to glucose after enzymatic hydrolysis and 80.3 ± 1.2% yield of monosaccharides (glucose plus xylose and arabinose) from cellulose and hemicellulose of wheat straw were achieved.


2014 ◽  
Vol 670-671 ◽  
pp. 240-243
Author(s):  
Ning Xu ◽  
Ben Lin Dai ◽  
Zhen Wu ◽  
Fei Hu Mu ◽  
Ji Ming Xu

Pretreatment is a critical step in the conversion of lignocellulose to fermentable sugars. In this work, Miscanthus straw was pretreated with ammonium carbonate (Na2CO3), which was shown by earlier works to be an effective pretreatment of grass stovers and other plant materials in the context of ethanol production. Here, we explored several key parameters including Na2CO3 concentration (2–6%), temperature (120–160°C), and reaction time (10–30 min) to evaluate their effects on enzymatic digestibility using response surface methodology. The best conditions were found to be Na2CO3 of 4.4%, temperature of 150.7°C, and reaction time of 20.0 min, under which glucose yield reached to 294.5 g/Kg biomass.


2012 ◽  
Vol 622-623 ◽  
pp. 117-121 ◽  
Author(s):  
Sairudee Duangwang ◽  
Chayanoot Sangwichien

Oil palm empty fruit bunch (OPEFB) is a potential source of cellulose which can serve as a promising raw material for the production of ethanol. The increasing interest on use of lignocellulosic waste for bioconversion to fuels and chemicals are justifiable as these materials are low cost, renewable and widespread sources of sugars. The objective of the present study was to determine the effect of NaOH concentration, reaction temperature and reaction time for alkali pretreatment to increase the amount of cellulose for hydrolysis to achieve high glucose yield. Batch reactions were carried out under various reaction temperature, reaction time and NaOH concentration. Response Surface Methodology (RSM) was followed to optimize the alkali pretreatment in order to obtain high amount of cellulose (%). The optimum reaction temperature, reaction time and NaOH concentration were found to be 130 ˚c, 40 min and 15% (w/v), respectively. The maximum value of cellulose was obtained 68.8% by using the above condition. 19.96 g/L glucose was achieved when reaction was carried out at 130 ˚c for time period of 90 min when 6% (w/v) of H2SO4 concentration maintained.


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