Production of gymnemic acid from hairy root cultures of Gymnema sylvestre R. Br. as influenced by polyunsaturated fatty acids (PUFAs) and their antioxidant activity

2014 ◽  
Vol 54 ◽  
pp. 54-61 ◽  
Author(s):  
N. Praveen ◽  
M. Thiruvengadam ◽  
Y.S. Yang ◽  
S.H. Kim ◽  
H.N. Murthy ◽  
...  
2013 ◽  
Vol 35 (10) ◽  
pp. 3067-3073 ◽  
Author(s):  
P. Nagella ◽  
M. Thiruvengadam ◽  
S. J. Jung ◽  
H. N. Murthy ◽  
I. M. Chung

2013 ◽  
Vol 61 (48) ◽  
pp. 11744-11758 ◽  
Author(s):  
Cesar Nopo-Olazabal ◽  
John Hubstenberger ◽  
Luis Nopo-Olazabal ◽  
Fabricio Medina-Bolivar

2010 ◽  
Vol 26 (5) ◽  
pp. 1344-1351 ◽  
Author(s):  
Julie A. Abbott ◽  
Fabricio Medina-Bolivar ◽  
Elizabeth M. Martin ◽  
Abigail S. Engelberth ◽  
Hector Villagarcia ◽  
...  

2010 ◽  
Vol 65 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Vasil Georgiev Georgiev ◽  
Jost Weber ◽  
Eva-Maria Kneschke ◽  
Petko Nedyalkov Denev ◽  
Thomas Bley ◽  
...  

2018 ◽  
Vol 23 ◽  
pp. 302-307
Author(s):  
N. A. Matvieieva ◽  
O. A. Havryliuk ◽  
L. S. Yastremska

Aim. The aim of the work was to investigate the effect of short-term cold stress (+10°С) on the growth, flavonoid synthesis and antioxidant activity in Artemisia vulgaris L. and A. annua L. “hairy“ root cultures. Methods. Transgenic roots were cultivated during the first 1, 2 and 5 days at +10°C on Murashige and Skoog basal medium with twice reduced macrosalt content. The total flavonoids content in Rutin equivalent was determined using alcohol extract reaction with alumunium chloride. Antioxidant activity was studied using the DPPH method. Results. Short-term cold stress resulted in a reduction of mass increment by 12–76 %. The total flavonoid content in «hairy» roots ranged from 32.0±3.13 to 187.0±21.04 mg RE/g dry weight. Decrease of temperature has led to decrease of the flavonoids content in No. 1, No. 2 A. vulgaris root lines and No. 5 A. annua line by 18–33 %. The reaction of No. 3 A. vulgaris and No. 4 A. annua root lines was expressed in stimulation flavonoid synthesis by 62 % and 56.5 %. Cultivation of «hairy» roots under short-term cold stress has led to decrease of the antioxidant activity in all roots lines by 4–40 %. Conclusions. Сold stress had negative effect the “hairy“ roots growth, stimulated flavonoids accumulation only in two “hairy” root lines and reduced the level of antioxidant activity. Keywords: «hairy» roots culture, Artemisia spp., cold stress, flavonoids, antioxidant activity.


Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
T Hasanloo ◽  
H Rahnama ◽  
R Sepehrifar ◽  
MR Shams

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