Ultrasound-assisted enzymatic extraction (UAEE) of phytochemical compounds from mulberry (Morus nigra) must and optimization study using response surface methodology

2015 ◽  
Vol 63 ◽  
pp. 214-225 ◽  
Author(s):  
William Tchabo ◽  
Yongkun Ma ◽  
Felix N. Engmann ◽  
Haining Zhang
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enzymatic Extraction ◽  
Morus Nigra ◽  
Optimization Study ◽  
Phytochemical Compounds ◽  
Ultrasound Assisted

scholarly journals Polysaccharides fromTrichosanthes Fructusvia Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Fujia Chen ◽  
Dahong Li ◽  
Hongqi Shen ◽  
Chunhong Wang ◽  
Enzhong Li ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Average Molecular Weight ◽  
Reducing Power ◽  
Extraction Temperature ◽  
Antitumor Activities ◽  
Enzymatic Extraction ◽  
Solid Ratio ◽  
Natural Medicine ◽  
Ultrasound Assisted

An efficient procedure for ultrasound-assisted enzymatic extraction of crude polysaccharides fromTrichosanthes Fructus(crude TFP) using response surface methodology (RSM) was developed. The Box–Behnken design was applied to optimize the effects of pH (X1), enzyme amount (X2), extraction temperature (X3), and liquid-to-solid ratio (X4) on the extraction. The statistical analysis indicated that the independent variables (X4,X2, andX3), the quadratic coefficients (X12,X22,X32, andX42), and the interaction coefficient (X1X3) had significant impact on the yield of crude TFP. The optimal conditions were determined as follows: pH 4.5, enzyme amount 5000 u/g, extraction temperature 45°C, and liquid-to-solid ratio 30 ml/g. The experimental yield of crude TFP was 6.58%, which was very close to the predicted yield of 6.71%. TFPI was then purified and characterized with Sephadex G-100 column, UV-Vis, GPC, and FT-IR. The average molecular weight of TFPI was calculated to be 1.49 × 105 Da. TFPI exhibited strong reducing power and possessed not only remarkable scavenging activities against ABTS•+and DPPH radicals, but also high antitumor activities in C4-2, DU145, and PC3 cells. The results suggest thatTrichosanthes Fructusand TFPI could be a novel potent natural medicine with antioxidant and antitumor activities.

Extraction of triterpenoids and phenolic compounds from Ganoderma lucidum: optimization study using the response surface methodology

2018 ◽  
Vol 9 (1) ◽  
pp. 209-226 ◽  
Author(s):  
Taofiq Oludemi ◽  
Lillian Barros ◽  
M. A. Prieto ◽  
Sandrina A. Heleno ◽  
Maria F. Barreiro ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Ganoderma Lucidum ◽  
Extraction Techniques ◽  
Ultrasound Assisted Extraction ◽  
Optimization Study ◽  
Assisted Extraction ◽  
Ultrasound Assisted

The extraction of triterpenoids and phenolic compounds from Ganoderma lucidum was optimized by response surface methodology, using heat and ultrasound assisted extraction techniques.

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