scholarly journals Developmental regulation of oocyte lipid intake through ‘patent’ follicular epithelium in Drosophila melanogaster

iScience ◽  
2021 ◽  
Vol 24 (4) ◽  
pp. 102275
Author(s):  
Sarayu Row ◽  
Yi-Chun Huang ◽  
Wu-Min Deng
Keyword(s):  
Drosophila Melanogaster ◽  
Developmental Regulation ◽  
Follicular Epithelium

scholarly journals Developmental regulation of oocyte lipid uptake via ‘patent’ follicular epithelium in Drosophila melanogaster

2020 ◽  
Author(s):  
Sarayu Row ◽  
Wu-Min Deng
Keyword(s):  
Transcription Factor ◽  
Drosophila Melanogaster ◽  
Spatial Pattern ◽  
Developmental Regulation ◽  
Model System ◽  
Follicular Epithelium ◽  
Lipid Uptake ◽  
Developmentally Regulated ◽  
Tissue Integrity ◽  
Evolutionarily Conserved

AbstractEpithelia form protective permeability barriers that selectively allow the exchange of material while maintaining tissue integrity under extreme mechanical, chemical, and bacterial loads. Here, we report in the Drosophila follicular epithelium a developmentally regulated and evolutionarily conserved process, ‘patency’, wherein a breach is created in the epithelium at tricellular contacts during mid-vitellogenesis. In Drosophila, patency exhibits a strict temporal range delimited by the transcription factor Tramtrack69, and a spatial pattern regulated by the dorsal-anterior signals of the follicular epithelium. Crucial for lipid uptake by the oocyte, patency is also exploited by endosymbionts such as Spiroplasma pulsonii. Our findings reveal an evolutionarily conserved non-typical epithelial function in a classic model system.

The 93D heat shock locus of Drosophila melanogaster: modulation by genetic and developmental factors

Genome ◽  
1989 ◽  
Vol 31 (2) ◽  
pp. 677-683 ◽  
Author(s):  
S. C. Lakhotia
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Developmental Regulation ◽  
Sequence Divergence ◽  
Unique Region ◽  
Reading Frame ◽  
Beta Alanine ◽  
Functional Conservation ◽  
Duplicate Loci ◽  
93D Locus

The 93D locus in Drosophila melanogaster and the 93D-like loci in other species of Drosophila, collectively termed hsr ω (heat shock RNA omega) locus, display several unique and intriguing features: (i) developmental regulation and selective induction by several agents like benzamide, colchicine, thiamphenicol, vit-B6; (ii) functional conservation in the genus but a very rapid DNA base sequence divergence; (iii) in spite of the rapid DNA sequence divergence, a strong conservation of organization (a 5′ unique region and a 3′ long tandem repeat region) and the pattern of multiple ω transcripts in the genus; (iv) a general nontranslatability of all the three major species of ω transcripts (an ~ 10-kb ω1, a 2.0-kb ω2, and a 1.2-kb ω3 species) although some recent evidence favours translatability of a small open reading frame (~ 23 – 27 amino acid long) in the ω3 transcript; (v) dispensability of the hsr ω locus for heat shock protein synthesis but indispensability for viability of flies. The heat shock inducibility of the 93D locus of D. melanogaster is selectively repressed by (i) combination of heat shock with another inducer of 93D; (ii) rearing of larvae at 10 °C; (iii) heterozygous deficiency for the 93D region; and (iv) conditions that alter levels of beta-alanine. In all cases of repression of the 93D locus during heat shock, the 87A and 87C loci (the two duplicate loci harbouring multiple copies for hsp70 and the alpha–beta repeat sequences (at 87C)) develop unequal puffs. The hsr ω locus appears to be under a complex system of regulation involving autoregulation as well as regulation by other factors in the cell which possibly operate through different control elements on the locus.Key words: benzamide, colchicine, beta-alanine, hsr ω, heat shock puffs, Drosophila.

scholarly journals Developmental regulation of an insulin-degrading enzyme from Drosophila melanogaster.

1988 ◽  
Vol 85 (10) ◽  
pp. 3469-3473 ◽  
Author(s):  
M. P. Stoppelli ◽  
J. V. Garcia ◽  
S. J. Decker ◽  
M. R. Rosner
Keyword(s):  
Drosophila Melanogaster ◽  
Developmental Regulation ◽  
Insulin Degrading Enzyme ◽  
Degrading Enzyme

scholarly journals Developmental regulation of N-terminal H2B methylation in Drosophila melanogaster

2011 ◽  
Vol 40 (4) ◽  
pp. 1536-1549 ◽  
Author(s):  
Ana Villar-Garea ◽  
Ignasi Forne ◽  
Irene Vetter ◽  
Elisabeth Kremmer ◽  
Andreas Thomae ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Developmental Regulation

Zygotic expression of the pebble locus is required for cytokinesis during the postblastoderm mitoses of Drosophila

Development ◽  
1992 ◽  
Vol 114 (1) ◽  
pp. 165-171 ◽  
Author(s):  
G. Hime ◽  
R. Saint
Keyword(s):  
Drosophila Melanogaster ◽  
Developmental Regulation ◽  
Single Cells ◽  
Embryonic Cell ◽  
Cell Cycles ◽  
Drosophila Embryogenesis ◽  
Zygotic Transcription ◽  
Homozygous Mutant ◽  
Mitotic Figures

Mutations at the pebble locus of Drosophila melanogaster result in embryonic lethality. Examination of homozygous mutant embryos at the end of embryogenesis revealed the presence of fewer and larger cells which contained enlarged nuclei. Characterization of the embryonic cell cycles using DAPI, propidium iodide, anti-tubulin and anti-spectrin staining showed that the first thirteen rapid syncytial nuclear divisions proceeded normally in pebble mutant embryos. Following cellularization, the postblastoderm nuclear divisions occurred (mitoses 14, 15 and 16), but cytokinesis was never observed. Multinucleate cells and duplicate mitotic figures were seen within single cells at the time of the cycle 15 mitoses. We conclude that zygotic expression of the pebble gene is required for cytokinesis following cellularization during Drosophila embryogenesis. We postulate that developmental regulation of zygotic transcription of the pebble gene is a consequence of the transition from syncytial to cellular mitoses during cycle 14 of embryogenesis.

scholarly journals Co-option of immune effectors by the hormonal signalling system triggering metamorphosis in Drosophila melanogaster

PLoS Genetics ◽  
2021 ◽  
Vol 17 (11) ◽  
pp. e1009916
Author(s):  
Catarina Nunes ◽  
Takashi Koyama ◽  
Élio Sucena
Keyword(s):  
Drosophila Melanogaster ◽  
Fat Body ◽  
Developmental Regulation ◽  
Bacterial Load ◽  
Systemic Effect ◽  
Dependent Manner ◽  
Downstream Target ◽  
Local Responses ◽  
Insect Metamorphosis

Insect metamorphosis is triggered by the production, secretion and degradation of 20-hydroxyecdysone (ecdysone). In addition to its role in developmental regulation, increasing evidence suggests that ecdysone is involved in innate immunity processes, such as phagocytosis and the induction of antimicrobial peptide (AMP) production. AMP regulation includes systemic responses as well as local responses at surface epithelia that contact with the external environment. At pupariation, Drosophila melanogaster increases dramatically the expression of three AMP genes, drosomycin (drs), drosomycin-like 2 (drsl2) and drosomycin-like 5 (drsl5). We show that the systemic action of drs at pupariation is dependent on ecdysone signalling in the fat body and operates via the ecdysone downstream target, Broad. In parallel, ecdysone also regulates local responses, specifically through the activation of drsl2 expression in the gut. Finally, we confirm the relevance of this ecdysone dependent AMP expression for the control of bacterial load by showing that flies lacking drs expression in the fat body have higher bacterial persistence over metamorphosis. In contrast, local responses may be redundant with the systemic effect of drs since reduction of ecdysone signalling or of drsl2 expression has no measurable negative effect on bacterial load control in the pupa. Together, our data emphasize the importance of the association between ecdysone signalling and immunity using in vivo studies and establish a new role for ecdysone at pupariation, which impacts developmental success by regulating the immune system in a stage-dependent manner. We speculate that this co-option of immune effectors by the hormonal system may constitute an anticipatory mechanism to control bacterial numbers in the pupa, at the core of metamorphosis evolution.

Closely related DNA sequences specify distinct patterns of developmental expression in Drosophila melanogaster

1990 ◽  
Vol 10 (6) ◽  
pp. 3272-3276
Author(s):  
H Xiao ◽  
J T Lis
Keyword(s):  
Drosophila Melanogaster ◽  
Dna Sequences ◽  
Developmental Regulation ◽  
Glucose Dehydrogenase ◽  
Regulatory Region ◽  
Transgenic Animals ◽  
Developmental Expression ◽  
Secretory Proteins ◽  
Regulatory Sequence ◽  
Hsp70 Gene

Three short synthetic DNA sequences, which are closely related to one another, confer three distinct patterns of developmental expression on the heat shock hsp70 gene in transgenic Drosophila melanogaster lines. These results show that small variations or even single base pair changes in a repeated element of a regulatory sequence can create promoters that display new specificities of tissue and developmental regulation. Interestingly, the three patterns of developmental expression conferred by the synthetic DNAs resemble in part those of the known developmental genes: glucose dehydrogenase (Gld), Dopa decarboxylase (Ddc), and salivary gland secretory proteins (Sgs), respectively. In each case, the defined regulatory region of the known developmental gene contains multiple sequences that are similar or identical to the synthetic sequence that confers a similar pattern of developmental expression on the hsp70 gene. Thus, these results are congruent with the view that short sequence elements in multiple copies can confer either simple or relatively complex patterns of developmental expression on a receptive promoter like that of hsp70. Furthermore, the fact that the three variants tested produced three distinct patterns of expression in transgenic animals suggests that the number of different elements is large.

scholarly journals Closely related DNA sequences specify distinct patterns of developmental expression in Drosophila melanogaster.

1990 ◽  
Vol 10 (6) ◽  
pp. 3272-3276 ◽  
Author(s):  
H Xiao ◽  
J T Lis
Keyword(s):  
Drosophila Melanogaster ◽  
Dna Sequences ◽  
Developmental Regulation ◽  
Glucose Dehydrogenase ◽  
Regulatory Region ◽  
Transgenic Animals ◽  
Developmental Expression ◽  
Secretory Proteins ◽  
Regulatory Sequence ◽  
Hsp70 Gene

Three short synthetic DNA sequences, which are closely related to one another, confer three distinct patterns of developmental expression on the heat shock hsp70 gene in transgenic Drosophila melanogaster lines. These results show that small variations or even single base pair changes in a repeated element of a regulatory sequence can create promoters that display new specificities of tissue and developmental regulation. Interestingly, the three patterns of developmental expression conferred by the synthetic DNAs resemble in part those of the known developmental genes: glucose dehydrogenase (Gld), Dopa decarboxylase (Ddc), and salivary gland secretory proteins (Sgs), respectively. In each case, the defined regulatory region of the known developmental gene contains multiple sequences that are similar or identical to the synthetic sequence that confers a similar pattern of developmental expression on the hsp70 gene. Thus, these results are congruent with the view that short sequence elements in multiple copies can confer either simple or relatively complex patterns of developmental expression on a receptive promoter like that of hsp70. Furthermore, the fact that the three variants tested produced three distinct patterns of expression in transgenic animals suggests that the number of different elements is large.

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