The Utility of Cervista Human Papillomavirus Assay in Necrotic Fine-needle Aspiration Specimens of Metastatic Squamous Cell Carcinoma from Neck Lymph Nodes

Introduction. Primary pulmonary lymphoepithelial carcinoma is a poorly differentiated squamous cell carcinoma admixed with variable amounts of lymphoplasmacytic infiltrate, frequently associated with EBV. It is a rare cancer and have a better prognosis than other non-small cell lung cancer. The tumor can mimic metastatic non-keratinizing SCC of the naso-pharynx, poorly differentiated non-small cell carcinoma and NUT carcinoma arising in the lung, and non-Hodgkin lymphomas. Materials and methods. A 75-year-old man presented with peripheral mass of left lower lobe, maximal diameter was 5.1 cm, involving lingula and medial pleura, with mediastinal, and retroperitoneal lymphadenopathy revealed by computer tomography. Fine needle aspiration-EBUS was performed from hilar and interlobar lymph nodes. Papanicolaou smears and cell blocks were prepared. Additional CT-guided cor-needle biopsy and FNA were performed from lung lesion later. Results. A few large malignant epithelial cells, consistent with non-small lung cancer, were found on a background of lymphocytes in fine-needle aspiration from lymph nodes. Immunostain results: Pan-CK (AE1/AE3), p63 and Ki67 were positive in malignant cells, leukocyte common antigen (CD45) was positive in lymphocytes (negative in tumor cells), and negative markers were TTF1, chromogranin and synaptophysin. Cytological diagnosis was metastatic non-small lung cancer, favor squamous cell carcinoma. Biopsy and aspiration from left lung showed syncytial groups of large malignant epithelial cells with scant cytoplasm and prominent nucleoli on a background of prominent inflammatory infiltrate,consistent with lymphoepithelial carcinoma. Conclusion. It is impossible to correctly diagnose metastasis of lymphoepithelial carcinoma in lymph node by FNA only without FNA or biopsy of primary lesion, because cytological features and immunostains are identical to non-keratinizing squamous cell carcinoma.

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p16 Immunostaining in Metastatic Squamous Cell Carcinoma Diagnosed on Fine Needle Aspiration of the Neck Lymph Node

Journal of the American Society of Cytopathology ◽

10.1016/j.jasc.2019.08.148 ◽

2019 ◽

Vol 8 (5) ◽

pp. S66

Author(s):

Tejal Patel ◽

Israh Akhtar ◽

Varsha Manucha

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Node ◽

Cell Carcinoma ◽

Squamous Cell ◽

Fine Needle Aspiration ◽

Needle Aspiration ◽

Neck Lymph Node ◽

Fine Needle ◽

Metastatic Squamous Cell Carcinoma

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Lymph Node Staging of Oral and Maxillofacial Neoplasms in 31 Dogs and Cats

Journal of Veterinary Dentistry ◽

10.1177/089875640201900301 ◽

2002 ◽

Vol 19 (3) ◽

pp. 122-126 ◽

Cited By ~ 64

Author(s):

Erin S. Herring ◽

Mark M. Smith ◽

John L. Robertson

Keyword(s):

Squamous Cell Carcinoma ◽

Lymph Node ◽

Lymph Nodes ◽

Cell Carcinoma ◽

Metastatic Disease ◽

Squamous Cell ◽

Fine Needle Aspiration ◽

Needle Aspiration ◽

Regional Lymph Nodes ◽

Fine Needle

A retrospective study was performed to report the histologic examination results of regional lymph nodes of dogs and cats with oral or maxillofacial neoplasms. Twenty-eight dogs and 3 cats were evaluated. Histologic examination results of standard and serial tissue sectioning of regional lymph nodes were recorded. When available, other clinical parameters including mandibular lymph node palpation, thoracic radiographs, and pre- and postoperative fine needle aspiration of lymph nodes were compared with the histologic results. Squamous cell carcinoma, fibrosarcoma, and melanoma were the most common neoplasms diagnosed in dogs. Squamous cell carcinoma and fibrosarcoma were diagnosed in cats. Of the palpably enlarged mandibular lymph nodes, 17.0 % had metastatic disease histologically. Radiographically evident thoracic metastatic disease was present in 7.4 % of cases. Preoperative cytologic evaluation of the mandibular lymph node based on fine needle aspiration concurred with the histologic results in 90.5 % of lymph nodes examined. Postoperative cytologic evaluation of fine needle aspirates of regional lymph nodes concurred with the histologic results in 80.6 % of lymph nodes examined. Only 54.5 % of cases with metastatic disease to regional lymph nodes had metastasis that included the mandibular lymph node. Serial lymph node sectioning provided additional information or metastasis detection. Cytologic evaluation of the mandibular lymph node correlates positively with histology, however results may fail to indicate the presence of regional metastasis. Assessment of all regional lymph nodes in dogs and cats with oral or maxillofacial neoplasms will detect more metastatic disease than assessing the mandibular lymph node only.

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