Purification of a synthetic oligonucleotide by anion exchange chromatography: Method optimisation and scale-up

2005 ◽  
Vol 64 (3) ◽  
pp. 216-225 ◽  
Author(s):  
Jamil Shanagar
Keyword(s):  
Anion Exchange ◽  
Scale Up ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Chromatography Method ◽  
Synthetic Oligonucleotide

Separation of Synthetic Oligonucleotide Dithioates from Monothiophosphate Impurities by Anion-Exchange Chromatography on a Mono-Q Column

2002 ◽  
Vol 306 (1) ◽  
pp. 92-99 ◽  
Author(s):  
Xianbin Yang ◽  
Richard P. Hodge ◽  
Bruce A. Luxon ◽  
Robert Shope ◽  
David G. Gorenstein
Keyword(s):  
Anion Exchange ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Synthetic Oligonucleotide

scholarly journals Separation of Sb (V) and Sb (III) antimony compounds using anion exchange chromatography technique

2020 ◽  
Vol 12 (3) ◽  
pp. 164-169
Author(s):  
Herlinawati Herlinawati ◽  
◽  
Buchari Buchari ◽  
M. Bachri Amran ◽  
◽  
...  
Keyword(s):  
Anion Exchange ◽  
Flow Rate ◽  
Phosphate Buffer ◽  
Mobile Phase ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Capacity Factor ◽  
Optimum Conditions ◽  
Chromatography Method ◽  
Theoretical Plates

Separation of Sb (V) and Sb (III) antimony compounds using anion exchange chromatography technique have been done. To obtain the separation of Sb (V) and Sb (III) antimony compounds which is good in this study have been studied several parameters of separation in anion exchange chromatography technique. Parameters that influence the process of separation of Sb (V) and Sb (III) antimony compounds is the concentration and pH of the mobile phase (eluent) have been evaluated. The separation of Sb (V) and Sb (III) antimony compounds is good and optimum obtained using an eluent 200 mM phosphate buffer at pH 7 with a flow rate of 1 mL/min. Based on the optimum conditions for the separation of Sb (V) and Sb (III) antimony compounds with anion exchange chromatography method has generated value the capacity factor (k ') Sb (V) and Sb (III) obtained are respectively 1.77 and 3.01. While the value of selectivity (α), Number of theoretical plates (N) and Resolution (Rs) obtained are respectively 1.70; 369.48; and 1.48.

Sign in / Sign up

Export Citation Format

Share Document