Isolation, characterization of melanin derived from Ophiocordyceps sinensis, an entomogenous fungus endemic to the Tibetan Plateau

2012 ◽  
Vol 113 (4) ◽  
pp. 474-479 ◽  
Author(s):  
Caihong Dong ◽  
Yijian Yao
2020 ◽  
Vol 15 (1) ◽  
Author(s):  
Jie Liu ◽  
Linong Guo ◽  
Zongwei Li ◽  
Zhe Zhou ◽  
Zhen Li ◽  
...  

Abstract Background Ophiocordyceps sinensis, which is only naturally found in the high-elevation extreme environment of the Tibetan Plateau, has been used in traditional Chinese medicine. Information concerning the evolutionary and geologic context of O. sinensis remains limited, however. Methods We constructed the high-quality genome of O. sinensis and provided insight into the evolution and ecology of O. sinensis using comparative genomics. Results We mapped the whole genome of the anamorph/asexual form Hirsutella of O. sinensis using Illumina and PacBio sequencing technologies and obtained a well assembled genome of 119.2 Mbp size. Long-read Single Molecule Real Time (SMRT) sequencing technology generated an assembly with more accurate representation of repeat sequence abundances and placement. Evolutionary analyses indicated that O. sinensis diverged from other fungi 65.9 Mya in the Upper Cretaceous, during the uplift of the Tibetan Plateau. Gene family expansions and contractions in addition to genome inflation via long terminal repeat (LTR) retrotransposon insertions were implicated as an important driver of O. sinensis divergence. The insertion rate of LTR sequences into the O. sinensis genome peaked ~ 30–40 Mya, when the Tibetan Plateau rose rapidly. Gene Ontology (GO) enrichment analysis suggested that O. sinensis contained more genes related to ice binding compared to other closely related fungi, which may aid in their adaptability to the cold Tibetan Plateau. Further, heavy metal resistance genes were in low abundance in the O. sinensis genome, which may help to explain previous observations that O. sinensis tissues contain high levels of heavy metals. Conclusions Our results reveal the evolutionary, geological, and ecological context for the evolution of the O. sinensis genome and the factors that have contributed to the environmental adaptability of this valuable fungus. These findings suggest that genome inflation via LTR retrotransposon insertions in O. sinensis coincided with the uplift of the Tibetan Plateau. LTRs and the specific genetic mechanisms of O. sinensis contributed to its adaptation to the environment on the plateau.


Oryx ◽  
2013 ◽  
Vol 48 (1) ◽  
pp. 30-38 ◽  
Author(s):  
Emily Woodhouse ◽  
Philip McGowan ◽  
E.J. Milner-Gulland

AbstractProvisioning ecosystem services include wild products that form an integral part of rural economies. Using quantitative and qualitative data from semi-structured interviews with 50 households in a Tibetan community in Western Sichuan, China, we explored the relationships of households with three diverse provisioning services on the Eastern Tibetan plateau: firewood, medicinal caterpillar fungus Ophiocordyceps sinensis and matsutake mushrooms Tricholoma matsutake. We examined (1) how they contribute to wealth and livelihoods, (2) what determines household access, and (3) how local use has changed over time. All households were reliant on firewood, and levels extracted were explained only by household size. A more complex set of factors explained access to caterpillar fungus: younger, larger, pastoralist households with lower dependency ratios tended to collect more, and education and household size explained variation in price gained for the product. Caterpillar fungus extraction has dramatically increased over the last 20 years, providing up to 72% of household income, but poorer households have received significantly less of their income from the fungus. Matsutake contributed much less to livelihoods because of its relatively low price. The results show a contrast between subsistence and market-driven products: access to the latter is affected by competition and power relationships. Overall access to provisioning services was related to facets of wealth, especially human capital. The study contributes a household level analysis of the diverse provisioning value of an under-researched part of the world, highlighting the heterogeneity and dynamism of the relationships of households with ecosystem services.


2001 ◽  
Vol 50 (3) ◽  
pp. 213-218 ◽  
Author(s):  
Toshitada FUJITA ◽  
Yoshifumi SOGI ◽  
Tetsuo YOSHIKAWA ◽  
Tomohiro NISHIO ◽  
Hiroshi TSUNO ◽  
...  

2012 ◽  
Vol 12 ◽  
pp. 103-110
Author(s):  
Bhushan Shrestha

Fungi are a part of the biodiversity that play a significant role in daily livelihood of the local communities. Yarsagumba (Ophiocordyceps sinensis) is one of the highly valued medicinal fungi that grow in the Tibetan Plateau of China and alpine grasslands of Nepal, Bhutan and India. Genus Cordyceps was recently revised and divided into four genera: Cordyceps, Elaphocordyceps, Metacordyceps and Ophiocordyceps, based on molecular phylogeny and morphology. The recent revision has consequently changed the scientific name of Yarsa gumba from Cordyceps sinensis (Berk.) Sacc. to Ophiocordyceps sinensis (Berk.) Sung et al. In Nepal, scientific study of Cordyceps species started about 60 years ago. During last 30 years, different Cordyceps species have been reported from Nepal. In this paper, Cordyceps species reported from Nepal have been discussed along with their synomyms, morphological characters, hosts and distributions in the global context.DOI: http://dx.doi.org/10.3126/njst.v12i0.6487 Nepal Journal of Science and Technology 12 (2011) 103-110 


2017 ◽  
Vol 206 ◽  
pp. 143-150 ◽  
Author(s):  
Yujing Yan ◽  
Yi Li ◽  
Wen-Jing Wang ◽  
Jin-Sheng He ◽  
Rui-Heng Yang ◽  
...  

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