Proteomic analysis of the effect of bile salts on the intestinal and probiotic bacterium Lactobacillus reuteri

2008 ◽  
Vol 137 (1-4) ◽  
pp. 14-19 ◽  
Author(s):  
KiBeom Lee ◽  
Hong-Gu Lee ◽  
Yun-Jaie Choi
Keyword(s):  
Proteomic Analysis ◽  
Bile Salts ◽  
Lactobacillus Reuteri ◽  
Probiotic Bacterium

The characteristics of lactic acid bacteria isolated from fermented food as potential probiotics

2021 ◽  
Vol 32 (4) ◽  
pp. 743-749
Author(s):  
Victoria Yulita Fitriani ◽  
Budi Suprapti ◽  
Muhammad Amin
Keyword(s):  
Lactobacillus Acidophilus ◽  
Bile Salts ◽  
Pathogenic Bacteria ◽  
Fruit Juice ◽  
Lactobacillus Reuteri ◽  
Antibacterial Properties ◽  
Fermented Food ◽  
Cow’S Milk ◽  
Cow's Milk

Abstract Objectives This study aims to determine the characteristics of Lactobacillus acidophilus and Lactobacillus reuteri from fermented soursop fruit juice and cow’s milk, respectively as probiotic candidate based on exposure to pH, bile salts, pathogenic bacteria, and antibiotics. Methods In vitro studies were conducted to examine the resistance of Lactobacillus acidophilus and Lactobacillus reuteri in pH 2, 2.5, 3.2, and 7.2, resistance to bile salts, resistance to pathogenic bacteria (Escherichia coli, Staphylococcus aureus and Enterococcus faecalis) and antituberculosis antibiotics. Results Viability of Lactobacillus acidophilus and Lactobacillus reuteri isolates remained unchanged (6.3 × 107 CFU/mL and 5.03 × 107 CFU/mL) at various acidic pH, and had a low survival rate in Ox gall 0.3% (bile salts). These isolates also showed antibacterial properties against pathogens in the gastrointestinal tract. Both of these bacteria are quite safe to be used together with ofloxacin, linezolid, moxifloxacin, and levofloxacin, antibiotic for tuberculosis therapy. Conclusions The results showed that Lactobacillus acidophilus and Lactobacillus reuteri from fermented soursop fruit juice and cow’s milk respectively fulfilled the characteristics of probiotic and could potentially be used as adjunct therapy in tuberculosis drug-resistance.

scholarly journals Complex responses to inflammatory oxidants by the probiotic bacterium Lactobacillus reuteri

2019 ◽  
Author(s):  
Poulami Basu Thakur ◽  
Abagail R. Long ◽  
Benjamin J. Nelson ◽  
Ranjit Kumar ◽  
Alexander F. Rosenberg ◽  
...  
Keyword(s):  
Immune System ◽  
Hypochlorous Acid ◽  
Lactobacillus Reuteri ◽  
Inflammatory Diseases ◽  
Small Heat Shock Protein ◽  
Probiotic Bacterium ◽  
Transcriptomic Response ◽  
E Coli ◽  
Anti Inflammatory ◽  
Associated Species

ABSTRACTInflammatory diseases of the gut are associated with increased intestinal oxygen concentrations and high levels of inflammatory oxidants, including hydrogen peroxide (H2O2) and hypochlorous acid (HOCl), which are antimicrobial compounds produced by the innate immune system. This contributes to dysbiotic changes in the gut microbiome, including increased populations of pro-inflammatory enterobacteria (Escherichia coli and related species) and decreased levels of health-associated anaerobic Firmicutes and Bacteroidetes. The pathways for H2O2 and HOCl resistance in E. coli have been well-studied, but little is known about how commensal and probiotic bacteria respond to inflammatory oxidants. In this work, we have characterized the transcriptomic response of the anti-inflammatory, gut-colonizing Gram-positive probiotic Lactobacillus reuteri to both H2O2 and HOCl. L. reuteri mounts distinct responses to each of these stressors, and both gene expression and survival were strongly affected by the presence or absence of oxygen. Oxidative stress response in L. reuteri required several factors not found in enterobacteria, including the small heat shock protein Lo18, polyphosphate kinase 2, and RsiR, an L. reuteri-specific regulator of anti-inflammatory mechanisms. These results raise the intriguing possibility of developing treatments for inflammatory gut diseases that could sensitize pro-inflammatory enterobacteria to killing by the immune system while sparing anti-inflammatory, health-associated species.IMPORTANCEIt is becoming increasingly clear that effective treatment of inflammatory gut diseases will require modulation of the gut microbiota. Preventing pro-inflammatory bacteria from blooming while also preserving anti-inflammatory and commensal species is a considerable challenge, but our results suggest that it may be possible to take advantage of differences in the way different species of gut bacteria resist inflammatory oxidants to accomplish this goal.

scholarly journals Bile salts and cholesterol induce changes in the lipid cell membrane of Lactobacillus reuteri

2003 ◽  
Vol 95 (1) ◽  
pp. 86-91 ◽  
Author(s):  
M.P. Taranto ◽  
M.L. Fernandez Murga ◽  
G. Lorca ◽  
G.F. Valdez
Keyword(s):  
Cell Membrane ◽  
Bile Salts ◽  
Lactobacillus Reuteri

Proteomic analysis of the response of Listeria monocytogenes to bile salts under anaerobic conditions

2013 ◽  
Vol 62 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Angela Payne ◽  
Ty B. Schmidt ◽  
Bindu Nanduri ◽  
Ken Pendarvis ◽  
Joseph R. Pittman ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Proteomic Analysis ◽  
Bile Salts ◽  
Anaerobic Conditions

scholarly journals Complex Responses to Hydrogen Peroxide and Hypochlorous Acid by the Probiotic Bacterium Lactobacillus reuteri

mSystems ◽  
2019 ◽  
Vol 4 (5) ◽  
Author(s):  
Poulami Basu Thakur ◽  
Abagail R. Long ◽  
Benjamin J. Nelson ◽  
Ranjit Kumar ◽  
Alexander F. Rosenberg ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Immune System ◽  
Stress Response ◽  
Hypochlorous Acid ◽  
Lactobacillus Reuteri ◽  
Probiotic Bacterium ◽  
Antimicrobial Compounds ◽  
Transcriptomic Response ◽  
Content Type ◽  
Anti Inflammatory

ABSTRACT Inflammatory diseases of the gut are associated with increased intestinal oxygen concentrations and high levels of inflammatory oxidants, including hydrogen peroxide (H2O2) and hypochlorous acid (HOCl), which are antimicrobial compounds produced by the innate immune system. This contributes to dysbiotic changes in the gut microbiome, including increased populations of proinflammatory enterobacteria (Escherichia coli and related species) and decreased levels of health-associated anaerobic Firmicutes and Bacteroidetes. The pathways for H2O2 and HOCl resistance in E. coli have been well studied, but little is known about how commensal and probiotic bacteria respond to inflammatory oxidants. In this work, we have characterized the transcriptomic response of the anti-inflammatory, gut-colonizing Gram-positive probiotic Lactobacillus reuteri to both H2O2 and HOCl. L. reuteri mounts distinct but overlapping responses to each of these stressors, and both gene expression and survival were strongly affected by the presence or absence of oxygen. Oxidative stress response in L. reuteri required several factors not found in enterobacteria, including the small heat shock protein Lo18, polyphosphate kinase 2, and RsiR, an L. reuteri-specific regulator of anti-inflammatory mechanisms. IMPORTANCE Reactive oxidants, including hydrogen peroxide and hypochlorous acid, are antimicrobial compounds produced by the immune system during inflammation. Little is known, however, about how many important types of bacteria present in the human microbiome respond to these oxidants, especially commensal and other health-associated species. We have now mapped the stress response to both H2O2 and HOCl in the intestinal lactic acid bacterium Lactobacillus reuteri.

Differential proteomic analysis of outer membrane enriched extracts of Bacteroides fragilis grown under bile salts stress

Anaerobe ◽  
2016 ◽  
Vol 39 ◽  
pp. 84-90 ◽  
Author(s):  
Renata F. Boente ◽  
Heidi Pauer ◽  
Deborah N.S. Silva ◽  
Joaquim Santos Filho ◽  
Vanessa Sandim ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Proteomic Analysis ◽  
Bile Salts ◽  
Bacteroides Fragilis

In vitro assessment of functional properties of lactic acid bacteria isolated from faecal microbiota of healthy dogs for potential use as probiotics

2013 ◽  
Vol 4 (3) ◽  
pp. 267-275 ◽  
Author(s):  
B.C. Silva ◽  
L.R.C. Jung ◽  
S.H.C. Sandes ◽  
L.B. Alvim ◽  
M.R.Q. Bomfim ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salts ◽  
Pathogenic Bacteria ◽  
Lactobacillus Reuteri ◽  
Cell Surface Hydrophobicity ◽  
Lactobacillus Paraplantarum ◽  
Production Of Hydrogen

Lactic acid bacteria were isolated and identified in the faeces of Chinese Crested and Yorkshire terrier pups and their probiotic features were investigated in vitro. Thirty seven isolates were identified as Lactobacillus or Enterococcus. Out of these isolates, 31 were lactic acid bacteria (LAB) and belonged to the species Lactobacillus reuteri (16/37; 43.3%), Lactobacillus animalis (7/37; 18.9%), Lactobacillus acidophilus (3/37; 8.1%), Lactobacillus sanfranciscensis (2/37; 5.4%), Lactobacillus murinus (2/37; 5.4%), and Lactobacillus paraplantarum (1/37; 2.7%), while six other LAB isolates were Enterococcus spp. (6/37; 16.2%). Strains were tested for resistance to gastric acidity (pH 2.5 for 3 h) and bile salts (0.3% ox gall), cell surface hydrophobicity by microbial adhesion to solvents, antagonism against pathogenic bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, Salmonella enterica serovar Typhimurium and Listeria monocytogenes), production of hydrogen peroxide, and antibiotic susceptibility. Thirty four strains were highly resistant to acidic conditions with slight (18 strains) to moderate (16 strains) growth inhibition by bile salts. Seven isolates had highly hydrophobic cellular surfaces and 28 strains exhibited strong antagonism against the bacterial pathogens tested, although 8 isolates tested against Leptospira interrogans had no effect on pathogen growth. All isolates produced low rates of hydrogen peroxide. Based on these results, two Lactobacillus strains showed promising probiotic-related features and merit investigation as probiotics for dogs.

Silac-based quantitative proteomic analysis of Lactobacillus reuteri CRL 1101 response to the presence of selenite and selenium nanoparticles

2019 ◽  
Vol 195 ◽  
pp. 53-65 ◽  
Author(s):  
Beatriz Gómez-Gómez ◽  
Teresa Pérez-Corona ◽  
Fernanda Mozzi ◽  
Micaela Pescuma ◽  
Yolanda Madrid
Keyword(s):  
Proteomic Analysis ◽  
Lactobacillus Reuteri ◽  
Selenium Nanoparticles ◽  
Quantitative Proteomic Analysis

Effect of glutathione on growth of the probiotic bacterium Lactobacillus reuteri

2011 ◽  
Vol 76 (4) ◽  
pp. 423-426 ◽  
Author(s):  
KiBeom Lee ◽  
Ho-Jin Kim ◽  
Beom-Seop Rho ◽  
Sang-Kee Kang ◽  
Yun-Jaie Choi
Keyword(s):  
Lactobacillus Reuteri ◽  
Probiotic Bacterium

scholarly journals Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach

2007 ◽  
Vol 73 (21) ◽  
pp. 6757-6767 ◽  
Author(s):  
Borja S�nchez ◽  
Marie-Christine Champomier-Verg�s ◽  
Birgitte Stuer-Lauridsen ◽  
Patricia Ruas-Madiedo ◽  
Patricia Anglade ◽  
...  
Keyword(s):  
Bile Salt ◽  
Resistant Strain ◽  
Bile Salts ◽  
Redox Status ◽  
Resistance Mechanisms ◽  
Probiotic Bacterium ◽  
Bacterial Survival ◽  
Bifidobacterium Animalis ◽  
Salt Response ◽  
Fermented Dairy

ABSTRACT Bile salts are natural detergents that facilitate the digestion and absorption of the hydrophobic components of the diet. However, their amphiphilic nature makes them very inhibitory for bacteria and strongly influences bacterial survival in the gastrointestinal tract. Adaptation to and tolerance of bile stress is therefore crucial for the persistence of bacteria in the human colonic niche. Bifidobacterium animalis subsp. lactis, a probiotic bacterium with documented health benefits, is applied largely in fermented dairy products. In this study, the effect of bile salts on proteomes of B. animalis subsp. lactis IPLA 4549 and its bile-resistant derivative B. animalis subsp. lactis 4549dOx was analyzed, leading to the identification of proteins which may represent the targets of bile salt response and adaptation in B. animalis subsp. lactis. The comparison of the wild-type and the bile-resistant strain responses allowed us to hypothesize about the resistance mechanisms acquired by the derivative resistant strain and about the bile salt response in B. animalis subsp. lactis. In addition, significant differences in the levels of metabolic end products of the bifid shunt and in the redox status of the cells were also detected, which correlate with some differences observed between the proteomes. These results indicate that adaptation and response to bile in B. animalis subsp. lactis involve several physiological mechanisms that are jointly dedicated to reduce the deleterious impact of bile on the cell's physiology.

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