Kinetics and substrate selectivity of a Triticum aestivum xylanase inhibitor (TAXI) resistant D11F/R122D variant of Bacillus subtilis XynA xylanase

2010 ◽  
Vol 146 (4) ◽  
pp. 207-214 ◽  
Author(s):  
Louise E. Rasmussen ◽  
Jens F. Sørensen ◽  
Anne S. Meyer
Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2557
Author(s):  
Dilara Maslennikova ◽  
Oksana Lastochkina

We evaluated the effect of endobacteria Bacillus subtilis (strain 10–4) as a co-inoculant for promoting plant growth and redox metabolism in two contrasting genotypes of Triticum aestivum L. (wheat): Ekada70 (drought tolerant (DT)) and Salavat Yulaev (drought susceptible (DS)) in early stages of adaptation to drought (12% PEG–6000). Results revealed that drought reduced growth and dramatically augmented oxidative stress markers, i.e., hydrogen peroxide (H2O2) and lipid peroxidation (MDA). Furthermore, the depletion of ascorbate (AsA) and glutathione (GSH), accompanied by a significant activation of ascorbate peroxidase (APX) and glutathione reductase (GR), in both stressed wheat cultivars (which was more pronounced in DS genotype) was found. B. subtilis had a protective effect on growth and antioxidant status, wherein the stabilization of AsA and GSH levels was revealed. This was accompanied by a decrease of drought-caused APX and GR activation in DS plants, while in DT plants additional antioxidant accumulation and GR activation were observed. H2O2 and MDA were considerably reduced in both drought-stressed wheat genotypes because of the application of B. subtilis. Thus, the findings suggest the key roles in B. subtilis-mediated drought tolerance in DS cv. Salavat Yulaev and DT cv. Ekada70 played are AsA and GSH, respectively; which, in both cases, resulted in reduced cell oxidative damage and improved growth in seedlings under drought.


2021 ◽  
Vol 12 (2) ◽  
pp. 87-95
Author(s):  
Agustín Yunuen Barajas-Vargas ◽  
Juan Luis Ignacio-De la Cruz ◽  
Liliana Márquez-Benavides ◽  
José Luis Hernández-Mendoza ◽  
Juan Manuel Sánchez-Yáñez

Author(s):  
Z. M. Kuramshina ◽  
R. M. Khairullin ◽  
Yu. V. Smirnova

In this study, we tested the effect of two strains of bacteria B. subtilis 26D and 11ВМ on three varieties of wheat Triticum aestivum L.: Omskaya 35, Kazakhstanskaya 10 (spring), Volzhskaya qualitative (winter).The peculiarity of the plants response to endophytic inoculation depended on the strain of the microorganism, the concentration of cells in the preparation, and the variety of wheat during the experiment in Petri dishes. Both strains showed a strong growth-stimulating effect when seed was inoculated with suspensions of bacteria with a concentration of 106 cells/ml. There was no effect when seed cells were inoculated with bacteria at a concentration of 109 cells / ml. Plants varieties Omskaya 35 were most responsive to inoculation with endophytes. The variety was well responsive to the inoculation of bacteria cells at different concentrations. The variety Volzhskaya quality had the least growth stimulation. Plants of this variety responded well when grown in soil, unlike experiments in Petri dishes. The variety Kazakhstanskaya 10 was less responsive when growing plants in Petri dishes. There was no difference between the size of the shoots of inoculated and non-inoculated plants of the variety Kazakh 10, only stimulation of root growth was observed. It was concluded that there is a pronounced responsiveness of wheat varieties to the effect of endophytic strains of bacteria B. subtilis 26D the basis of biofungicide (Fitosporin-M) and this must be considered when using biofungicide for wheat cultivation.


2009 ◽  
Vol 24 (3) ◽  
pp. 646-654 ◽  
Author(s):  
Ellen Fierens ◽  
Kurt Gebruers ◽  
Arnout R.D. Voet ◽  
Marc De Maeyer ◽  
Christophe M. Courtin ◽  
...  

2003 ◽  
Vol 23 (2-6) ◽  
pp. 249-255 ◽  
Author(s):  
Makoto Ashiuchi ◽  
Hisaaki Nakamura ◽  
Takashi Yamamoto ◽  
Tohru Kamei ◽  
Kenji Soda ◽  
...  

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