Simultaneous determination of tryptophan and kynurenine in plasma samples of children patients with Kawasaki disease by high-performance liquid chromatography with programmed wavelength ultraviolet detection

2009 ◽  
Vol 877 (16-17) ◽  
pp. 1678-1682 ◽  
Author(s):  
Xiaoqing Zhang ◽  
Yun He ◽  
Min Ding
2007 ◽  
Vol 90 (3) ◽  
pp. 872-878 ◽  
Author(s):  
Jinhui Zhou ◽  
Jianzhong Shen ◽  
Xiaofeng Xue ◽  
Jing Zhao ◽  
Yi Li ◽  
...  

Abstract A rapid and sensitive high-performance liquid chromatography (LC) method was developed for the simultaneous determination of metronidazole (MNZ), dimetridazole (DMZ), ronidazole (RNZ), tinidazole (TNZ), and 2-hydroxymethyl-1-methyl-5-nitroimidazole (HMMNI) in honey. After extraction with ethyl acetate and evaporation, the residue containing the nitroimidazoles was dissolved in ethyl acetatehexane and subjected to solid-phase extraction cleanup by amine extraction columns. The effluent was evaporated to dryness, and residues were dissolved and determined by LC with an ultraviolet detector set at 315 nm. The limits of detection were 1.02.0 ng/g for MNZ, DMZ, RNZ, TNZ, and HMMNI in honey. Average recoveries ranged from 71.5101.4% in honey fortified at 10, 20, 50, and 100 ng/g. The method was validated for the analysis of real honey samples.


2015 ◽  
Vol 7 (21) ◽  
pp. 9206-9210 ◽  
Author(s):  
Yunjeong Kim ◽  
Na Ha ◽  
Min-Gul Kim

A method combining high-performance liquid chromatography with ultraviolet detection (HPLC-UV) was developed and validated for the accurate determination ofl-ascorbic acid (AA) and dehydroascorbic acid (DHAA) concentrations in human plasma.


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