Simultaneous determination of phytohormones containing carboxyl in crude extracts of fruit samples based on chemical derivatization by capillary electrophoresis with laser-induced fluorescence detection

2011 ◽  
Vol 879 (20) ◽  
pp. 1802-1808 ◽  
Author(s):  
Hao Chen ◽  
Xiao-Feng Guo ◽  
Hua-Shan Zhang ◽  
Hong Wang
Keyword(s):  
Capillary Electrophoresis ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
Laser Induced Fluorescence ◽  
Chemical Derivatization ◽  
Laser Induced Fluorescence Detection ◽  
Crude Extracts ◽  
Fruit Samples

scholarly journals Optimisation of analytical conditions for simultaneous determination of phenol and chlorophenols by capillary electrophoresis with indirect laser-induced fluorescence detection

2021 ◽  
Vol 63 (11) ◽  
pp. 51-55
Author(s):  
Manh Huy Nguyen ◽  
◽  
Hong Anh Duong ◽  
Hung Viet Pham ◽  
◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Phenolic Compounds ◽  
Simultaneous Determination ◽  
Fluorescence Detection ◽  
Background Electrolyte ◽  
Laser Induced Fluorescence ◽  
Laser Induced Fluorescence Detection ◽  
Electrophoresis Method ◽  
Wastewater Samples

This work presented a capillary electrophoresis method with indirect laser-induced fluorescence detection for the simultaneous determination of phenol and four chlorophenol derivatives. The separation was obtained within 20 minutes with a background electrolyte composed of 5 mM borax and 1 mM fluorescein (pH=9.75), 17 kV of applied voltage, and 120 s of hydrostatic injection. At the optimal conditions, the limit of detections for phenolic compounds was in the range of 0.08-0.23 mg/l, and the RSD data of repeatability and reproducibility were less than 8.0% for both migration times and peak areas. This developed method was applied to analyse concentrations of phenolic compounds in surface water and wastewater samples, with the recoveries ranging from 59.4 to 102.5%.

Sign in / Sign up

Export Citation Format

Share Document