Separation and purification of water-soluble iridoid glucosides by high speed counter-current chromatography combined with macroporous resin column separation

2013 ◽  
Vol 936 ◽  
pp. 57-62 ◽  
Author(s):  
Hui-Lan Yue ◽  
Xiao-Hui Zhao ◽  
Qi-Lan Wang ◽  
Yan-Duo Tao
Keyword(s):  
High Speed ◽  
Water Soluble ◽  
Separation And Purification ◽  
Macroporous Resin ◽  
Resin Column ◽  
Column Separation ◽  
Iridoid Glucosides ◽  
Counter Current ◽  
Purification Of Water

scholarly journals Isolation and Identification of Anthocyanin Component in the Fruits of Acanthopanax Sessiliflorus (Rupr. & Maxim.) Seem. by Means of High Speed Counter Current Chromatography and Evaluation of Its Antioxidant Activity

Molecules ◽  
2020 ◽  
Vol 25 (8) ◽  
pp. 1781 ◽  
Author(s):  
Liang Chen ◽  
Xiulan Xin ◽  
Hui Feng ◽  
Shuangshi Li ◽  
Qiguang Cao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Speed ◽  
Radical Scavenging ◽  
Ionization Mass ◽  
Anthocyanin Content ◽  
Separation And Purification ◽  
Isolation And Identification ◽  
Antioxidant Power ◽  
Acanthopanax Sessiliflorus ◽  
Counter Current

Acanthopanax sessiliflorus (Rupr. & Maxim.) Seem. (Araliaceae) is one of the most abundant species of genus Acanthopanax. The fruits of A. sessiliflorus are used in traditional medical protocols as an analgesic, tonic, antidiabetic, antihypertensive, anti-inflammatory, antitumor, and immune-stimulating agent. In this work, we carried out a comprehensive investigation into the anthocyanin components in the fruits of A. sessiliflorus. The anthocyanin content in the fresh fruits of A. sessiliflorus was determined by high performance liquid chromatography-diode array detection (HPLC/DAD), and the anthocyanin component was isolated from these using high-speed counter-current chromatography (HSCCC) and elucidated by electro-spray ionization-mass spectrometry (ESI/MS), 1H- and 13C-NMR. Its antioxidant activity was evaluated by ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). We found that A. sessiliflorus contained a gross anthocyanin content of 121.35 mg/100 g. HSCCC was successfully used for separation and purification of the primary anthocyanin component, cyanidin 3-xylosyl-galactoside. The antioxidant and radical scavenging tests indicated that cyanidin 3-xylosyl-galactoside is a potent antioxidant.

Sign in / Sign up

Export Citation Format

Share Document