Peptide discs as precursors of biologically relevant supported lipid bilayers

2021 ◽  
Vol 585 ◽  
pp. 376-385
Author(s):  
Alessandra Luchini ◽  
Federica Sebastiani ◽  
Frederik Grønbæk Tidemand ◽  
Krishna Chaithanya Batchu ◽  
Mario Campana ◽  
...  
2015 ◽  
Vol 108 (2) ◽  
pp. 542a
Author(s):  
Souryvanh Nirasay ◽  
Antonella Badia ◽  
Jerome P. Claverie ◽  
Isabelle Marcotte

Coatings ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 981
Author(s):  
Palak Sondhi ◽  
Dhanbir Lingden ◽  
Keith J. Stine

Biomimetic membrane systems play a crucial role in the field of biosensor engineering. Over the years, significant progress has been achieved creating artificial membranes by various strategies from vesicle fusion to Langmuir transfer approaches to meet an ever-growing demand for supported lipid bilayers on various substrates such as glass, mica, gold, polymer cushions, and many more. This paper reviews the diversity seen in the preparation of biologically relevant model lipid membranes which includes monolayers and bilayers of phospholipid and other crucial components such as proteins, characterization techniques, changes in the physical properties of the membranes during molecular interactions and the dynamics of the lipid membrane with biologically active molecules with special emphasis on lipopolysaccharides (LPS).


2018 ◽  
Author(s):  
Luke Jordan ◽  
Nathan Wittenberg

This is a comprehensive study of the effects of the four major brain gangliosides (GM1, GD1b, GD1a, and GT1b) on the adsorption and rupture of phospholipid vesicles on SiO2 surfaces for the formation of supported lipid bilayer (SLB) membranes. Using quartz crystal microbalance with dissipation monitoring (QCM-D) we show that gangliosides GD1a and GT1b significantly slow the SLB formation process, whereas GM1 and GD1b have smaller effects. This is likely due to the net ganglioside charge as well as the positions of acidic sugar groups on ganglioside glycan head groups. Data is included that shows calcium can accelerate the formation of ganglioside-rich SLBs. Using fluorescence recovery after photobleaching (FRAP) we also show that the presence of gangliosides significantly reduces lipid diffusion coefficients in SLBs in a concentration-dependent manner. Finally, using QCM-D and GD1a-rich SLB membranes we measure the binding kinetics of an anti-GD1a antibody that has similarities to a monoclonal antibody that is a hallmark of a variant of Guillain-Barre syndrome.


2009 ◽  
pp. 5100 ◽  
Author(s):  
Juewen Liu ◽  
Alison Stace-Naughton ◽  
C. Jeffrey Brinker

Langmuir ◽  
2021 ◽  
Author(s):  
Hanna Ulmefors ◽  
Josefin Nissa ◽  
Hudson Pace ◽  
Olov Wahlsten ◽  
Anders Gunnarsson ◽  
...  

2012 ◽  
Vol 1 (3) ◽  
pp. 348-353 ◽  
Author(s):  
Katharine Epler ◽  
David Padilla ◽  
Genevieve Phillips ◽  
Peter Crowder ◽  
Robert Castillo ◽  
...  

2021 ◽  
Vol 120 (3) ◽  
pp. 146a
Author(s):  
Autumn A. Anthony ◽  
Aurelia R. Honerkamp-Smith

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