Emperipolesis-like Invasion of Neoplastic Lymphocytes into Hepatocytes in Feline T-cell Lymphoma

2011 ◽  
Vol 144 (4) ◽  
pp. 312-316 ◽  
Author(s):  
M. Suzuki ◽  
Y. Kanae ◽  
Y. Kagawa ◽  
N. Ano ◽  
K. Nomura ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Lymphoma ◽  
T Cell Lymphoma ◽  
Neoplastic Lymphocytes

Immunophenotyping of intraepithelial lymphocytes in canine chronic enteropathy and intestinal T-cell lymphoma using endoscopic samples

2021 ◽  
pp. 030098582110572
Author(s):  
Kazuhiro Kojima ◽  
James K. Chambers ◽  
Ko Nakashima ◽  
Yuko Goto-Koshino ◽  
Kazuyuki Uchida
Keyword(s):  
T Cell ◽  
Cell Lymphoma ◽  
Intraepithelial Lymphocytes ◽  
T Cell Lymphoma ◽  
Double Labeling ◽  
Chronic Enteropathy ◽  
High Proliferative Activity ◽  
Neoplastic Lymphocytes ◽  
Mass Lesions ◽  
Intraepithelial Lymphocytosis

Human enteropathy-associated T-cell lymphoma (EATL) is considered to be derived from intraepithelial lymphocytes (IELs); however, the origin of canine intestinal T-cell lymphoma (ITCL) remains unclear. Histological, immunohistochemical, and clonality examinations were performed using endoscopically collected canine duodenum samples of mucosal lesions of chronic enteropathy (CE; 73 cases) and ITCL without transmural neoplastic mass lesions (64 cases). Histopathological examinations revealed the intraepithelial accumulation of lymphocytes (called “intraepithelial lymphocytosis”) in 54/73 CE cases (74%) and the epitheliotropism of neoplastic lymphocytes in 63/64 ITCL cases (98%). Immunohistochemically, IELs in CE with intraepithelial lymphocytosis (IEL+CE) were diffusely immunopositive for CD3, with scattered immunopositivity for CD5, CD8, CD20, and granzyme B (GRB). The percentage of CD8+ in CD3+ IELs was significantly lower in IEL+CE than in CE without intraepithelial lymphocytosis (IEL−CE). Double-labeling immunohistochemistry revealed a high percentage of GRB expression in CD8− IEL among IEL+CE. Among 64 ITCL cases, CD3 was immunopositive in 64 (100%), CD5 in 22 (34%), CD8 in 8 (13%), CD20 in 12 (19%), CD30 in 13 (20%), and GRB in 49 (77%). In CD3+ cells, Ki67 immunopositivity was highest in ITCL, intermediate in IEL+CE, and lower in IEL−CE. A clonal TCR gene rearrangement was detected in 1/19 IEL−CE cases (5%), 15/54 IEL+CE (28%), and 38/58 ITCL (66%). These results indicate that the immunophenotype of canine ITCL (CD8−GRB+) is similar to that of the increased IELs in CE. The high proliferative activity and clonality of T cells in IEL+CE suggest that canine ITCL originates from these IELs, similar to human EATL.

scholarly journals Epitheliotropic T-cell lymphoma in 2 half-sibling bontebok

2020 ◽  
pp. 104063872098411
Author(s):  
Sierra M. Imanse ◽  
Colleen F. Monahan ◽  
Kimberly A. Thompson ◽  
Judilee C. Marrow ◽  
Sarah M. Corner
Keyword(s):  
T Cell ◽  
Fibrous Material ◽  
Cell Lymphoma ◽  
Abdominal Distension ◽  
Neoplastic Cell ◽  
T Cell Lymphoma ◽  
History Of ◽  
Neoplastic Lymphocytes ◽  
Particle Length ◽  
Half Sibling

We diagnosed epitheliotropic T-cell lymphoma of the forestomachs in 2 aged, half-sibling, zoo-managed bontebok ( Damaliscus pygargus pygargus). One bontebok also had mesenteric lymph node and cutaneous involvement. Both animals had a history of chronic abdominal distension and diminished body condition that resulted in euthanasia. At autopsy, both animals had marked ruminal distension with diffusely blunted ruminal papillae and reticular crests. In case 1, there was an increased amount and particle length of the ruminoreticular fibrous material with scant fluid, and a 2-cm diameter focus of cutaneous crusting adjacent to a mammary teat. In case 2, the rumen and reticulum were fluid-distended with decreased fibrous material. Histologically in case 1, the rumen, reticulum, omasum, and skin had intraepithelial nests and sheets of neoplastic small lymphocytes; in case 2, the rumen and reticulum had a similar neoplastic cell population. Immunohistochemically, neoplastic lymphocytes were immunoreactive for CD3 and negative for CD20, confirming the diagnosis of epitheliotropic T-cell lymphoma.

scholarly journals Immunohistochemical Profiling of Canine Intestinal T-Cell Lymphomas

2018 ◽  
Vol 56 (1) ◽  
pp. 50-60 ◽  
Author(s):  
Isao Matsumoto ◽  
Ko Nakashima ◽  
Yuko Goto-Koshino ◽  
James Kenn Chambers ◽  
Hajime Tsujimoto ◽  
...  
Keyword(s):  
T Cell ◽  
Plasma Cells ◽  
Cell Transformation ◽  
Cell Lymphoma ◽  
Large Cell ◽  
T Cell Lymphoma ◽  
Type I ◽  
Type Ii ◽  
T Cell Lymphomas ◽  
Neoplastic Lymphocytes

Human enteropathy-associated T-cell lymphoma (EATL) is classified into 2 distinct subgroups based on their phenotypes (type I and type II). Canine intestinal T-cell lymphoma can be morphologically classified into large and small cell lymphomas (LCL and SCL, respectively). Their association with human EATL or immunohistochemical and biological features has not been well characterized. In this study, the immunohistochemical profiles of 17 cases of LCL and 33 cases of SCL were evaluated with markers used for human EATL classification. Morphologically, LCL was characterized by sheet-like proliferation of large to moderately sized neoplastic lymphocytes, with scant clear cytoplasm and pleomorphic, irregularly shaped nuclei containing distinctive nucleoli and scattered chromatin. In contrast, SCL was characterized by the proliferation of monomorphic small neoplastic lymphocytes, accompanied by infiltration of nonneoplastic plasma cells. Interestingly, 8 cases demonstrated mixed LCL and SCL morphologies. Granular cytoplasmic expression of granzyme B was observed in most LCL and SCL cases. Membranous expression of CD56 was demonstrated in only 2 of 17 LCL and 0 of 33 SCL cases. Coexpression of CD20 by neoplastic T cells was observed in more SCL cases (16/33; 48%) than LCL cases (1/17; 6%). The CD56-positive cells in 2 cases were negative for CD20. Although canine LCL shares common features with human EATL type I, canine SCL cells and human EATL type II differ in their immunophenotype. Canine intestinal T-cell lymphoma had a homogeneous immunophenotype regardless of cell morphology. The findings of this study may indicate large cell transformation of SCL rather than 2 distinct entities.

scholarly journals Disseminated T-cell lymphoma in a bonobo (Pan paniscus)

2011 ◽  
Vol 24 (1) ◽  
pp. 238-240 ◽  
Author(s):  
Katherine N. Gibson-Corley ◽  
Joseph S. Haynes
Keyword(s):  
T Cell ◽  
Pan Paniscus ◽  
Cell Lymphoma ◽  
Vena Cava ◽  
T Cell Lymphoma ◽  
Tissue Mass ◽  
Thoracic Inlet ◽  
Facial Swelling ◽  
Neoplastic Lymphocytes ◽  
Cluster Of Differentiation

Disseminated lymphoma was diagnosed in an 8-year-old male bonobo ( Pan paniscus). The male bonobo presented with a 4–6 week history of dyspnea and facial swelling around the eyes; thoracic radiographs and computed tomography scan indicated a craniodorsal mediastinal soft tissue mass. Upon gross examination, there was a large, cream to white mass expanding the mediastinum and pericardial sac. The mass extended along the thoracic aorta and cranial vena cava, through the thoracic inlet, along and encircling the trachea, and bilaterally into the thyroid glands. Microscopically, neoplastic lymphocytes were present in the thymus, trachea, lungs, kidney, heart, and numerous other tissues. Immunohistochemical staining of neoplastic lymphocytes revealed diffuse immunoreactivity for cluster of differentiation (CD)3 indicating T-cell lymphoma. Routine viral screening was negative via polymerase chain reaction.

Sign in / Sign up

Export Citation Format

Share Document