In situ observation of elementary growth steps on the surface of protein crystals by laser confocal microscopy

2004 ◽  
Vol 262 (1-4) ◽  
pp. 536-542 ◽  
Author(s):  
Gen Sazaki ◽  
Takuro Matsui ◽  
Katsuo Tsukamoto ◽  
Noritaka Usami ◽  
Toru Ujihara ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Protein Crystals ◽  
In Situ Observation ◽  
Laser Confocal Microscopy

Highly Purified Glucose Isomerase Crystals Under Microgravity Conditions Grow as Fast as Those on the Ground Do

2020 ◽  
Author(s):  
Yoshihisa Suzuki
Keyword(s):  
Growth Rates ◽  
Glucose Isomerase ◽  
Spiral Growth ◽  
Protein Crystals ◽  
In Situ Observation ◽  
Lateral Growth ◽  
Impurity Incorporation ◽  
Microgravity Conditions

<p>Suppression of convection flows (solute transportation) and that of impurity incorporation into crystals seem to be the main reasons why the quality of protein crystals becomes better under microgravity conditions, whereas each precise mechanism has not been completely clarified yet. We tried to clarify the former reason by the in-situ observation of spiral growth hillocks on the {110} faces of highly purified glucose isomerase (GI) crystals under microgravity conditions and on the ground. Lateral growth rates <i>V</i><sub>lateral</sub> of a spiral hillock on the {110} face of a glucose isomerase crystal in situ under microgravity conditions and step velocities <i>V</i><sub>step</sub> of the same configuration on the ground took similar values as far as the maximum values are compared each other. This similarity indicates there are less influences of the convection flows on the growth rates of protein crystals contrary to conventional expectations.</p>

scholarly journals In Situ Observation of Elementary Growth Processes of Protein Crystals by Advanced Optical Microscopy

2012 ◽  
Vol 19 (7) ◽  
pp. 743-760 ◽  
Author(s):  
Gen Sazaki ◽  
Alexander E.S. Van Driessche ◽  
Guoliang Dai ◽  
Masashi Okada ◽  
Takuro Matsui ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Protein Crystals ◽  
In Situ Observation ◽  
Growth Processes

In situ observation of iron ore particle reduction above 1373 K by confocal microscopy

2018 ◽  
Vol 26 (1) ◽  
pp. 32-41 ◽  
Author(s):  
Lei Guo ◽  
Jin-tao Gao ◽  
Sheng-ping Zhong ◽  
Qi-peng Bao ◽  
Zhan-cheng Guo
Keyword(s):  
Confocal Microscopy ◽  
Iron Ore ◽  
In Situ Observation

Highly Purified Glucose Isomerase Crystals Under Microgravity Conditions Grow as Fast as Those on the Ground Do

2020 ◽  
Author(s):  
Yoshihisa Suzuki
Keyword(s):  
Growth Rates ◽  
Glucose Isomerase ◽  
Spiral Growth ◽  
Protein Crystals ◽  
In Situ Observation ◽  
Lateral Growth ◽  
Impurity Incorporation ◽  
Microgravity Conditions

<p>Suppression of convection flows (solute transportation) and that of impurity incorporation into crystals seem to be the main reasons why the quality of protein crystals becomes better under microgravity conditions, whereas each precise mechanism has not been completely clarified yet. We tried to clarify the former reason by the in-situ observation of spiral growth hillocks on the {110} faces of highly purified glucose isomerase (GI) crystals under microgravity conditions and on the ground. Lateral growth rates <i>V</i><sub>lateral</sub> of a spiral hillock on the {110} face of a glucose isomerase crystal in situ under microgravity conditions and step velocities <i>V</i><sub>step</sub> of the same configuration on the ground took similar values as far as the maximum values are compared each other. This similarity indicates there are less influences of the convection flows on the growth rates of protein crystals contrary to conventional expectations.</p>

scholarly journals In situ Observation of Calcium Oxide Treatment of Inclusions in Molten Steel by Confocal Microscopy

2017 ◽  
Vol 48 (3) ◽  
pp. 1409-1415 ◽  
Author(s):  
Bharat Khurana ◽  
Stephen Spooner ◽  
M. B. V. Rao ◽  
Gour Gopal Roy ◽  
Prakash Srirangam
Keyword(s):  
Confocal Microscopy ◽  
Calcium Oxide ◽  
Molten Steel ◽  
In Situ Observation

In Situ Observation of Dislocations in Protein Crystals during Growth by Advanced Optical Microscopy

2005 ◽  
Vol 5 (5) ◽  
pp. 1729-1735 ◽  
Author(s):  
Gen Sazaki ◽  
Katsuo Tsukamoto ◽  
Satomi Yai ◽  
Masashi Okada ◽  
Kazuo Nakajima
Keyword(s):  
Optical Microscopy ◽  
Protein Crystals ◽  
In Situ Observation

In Situ Observation of Catalyzed Gasification of Graphite by Nickel

1981 ◽  
Vol 39 ◽  
pp. 76-77
Author(s):  
R. T. K. Baker ◽  
R. D. Sherwood
Keyword(s):  
Objective Lens ◽  
In Situ Observation ◽  
Gas Flows ◽  
Catalytic Gasification ◽  
Television Camera ◽  
Viewing Screen ◽  
Fuels And Chemicals ◽  
Gasification Of Carbon ◽  
Transmission Microscope

The catalytic gasification of carbon at high temperature by microscopic size metal particles is of fundamental importance to removal of coke deposits and conversion of refractory hydrocarbons into fuels and chemicals. The reaction of metal/carbon/gas systems can be observed by controlled atmosphere electron microscopy (CAEM) in an 100 KV conventional transmission microscope. In the JEOL gas reaction stage model AGl (Fig. 1) the specimen is positioned over a hole, 200μm diameter, in a platinum heater strip, and is interposed between two apertures, 75μm diameter. The control gas flows across the specimen and exits through these apertures into the specimen chamber. The gas is further confined by two apertures, one in the condenser and one in the objective lens pole pieces, and removed by an auxiliary vacuum pump. The reaction zone is <1 mm thick and is maintained at gas pressure up to 400 Torr and temperature up to 1300<C as measured by a Pt-Pt/Rh 13% thermocouple. Reaction events are observed and recorded on videotape by using a Philips phosphor-television camera located below a hole in the center of the viewing screen. The overall resolution is greater than 2.5 nm.

In situ observation of the martensitic transformation in (Mg,Y)-PSZ

1986 ◽  
Vol 44 ◽  
pp. 500-501
Author(s):  
R-R. Lee
Keyword(s):  
Martensitic Transformation ◽  
High Strength ◽  
Nucleation And Growth ◽  
In Situ Observation ◽  
Considerable Potential ◽  
Transmission Electron ◽  
Structural Applications ◽  
Applied Stresses ◽  
Kinetics Of

Partially-stabilized ZrO2 (PSZ) ceramics have considerable potential for advanced structural applications because of their high strength and toughness. These properties derive from small tetragonal ZrO2 (t-ZrO2) precipitates in a cubic (c) ZrO2 matrix, which transform martensitically to monoclinic (m) symmetry under applied stresses. The kinetics of the martensitic transformation is believed to be nucleation controlled and the nucleation is always stress induced. In situ observation of the martensitic transformation using transmission electron microscopy provides considerable information about the nucleation and growth aspects of the transformation.

Sign in / Sign up

Export Citation Format

Share Document