Implementation of an in-house real-time reverse transcription-PCR assay for the rapid detection of the SARS-CoV-2 Marseille-4 variant

Since 2013, group A rotavirus strains characterized as novel DS-1-like inter-genogroup reassortant ‘equine-like G3’ strains have emerged and spread across five continents among human populations in at least 14 countries. Here we report a novel one-step TaqMan quantitative real-time reverse transcription-PCR assay developed to genotype and quantify the viral load for samples containing rotavirus equine-like G3 strains. Using a universal G forward primer and a newly designed reverse primer and TaqMan probe, we developed and validated an assay with a linear dynamic range of 2.3 × 10 9 – 227 copies per reaction and a limit of detection of 227 copies. The percent positive agreement, percent negative agreement, and precision of our assay were 100.00%, 99.63%, and 100.00%, respectively. This assay can simultaneously detect and quantify the viral load for samples containing DS-1-like inter-genogroup reassortant equine-like G3 strains with high sensitivity and specificity, faster turnaround time, and decreased cost and will be valuable for high-throughput screening of stool samples collected to monitor equine-like G3 strain prevalence and circulation among human populations throughout the world.

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Development and validation of a real-time, reverse transcription PCR assay for rapid and low-cost genotyping of hepatitis C virus genotypes 1a, 1b, 2, and 3a

Journal of Virological Methods ◽

10.1016/j.jviromet.2017.02.009 ◽

2017 ◽

Vol 244 ◽

pp. 17-22 ◽

Cited By ~ 2

Author(s):

Andrea D. Olmstead ◽

Tracy D. Lee ◽

Ron Chow ◽

Kingsley Gunadasa ◽

Brian Auk ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Real Time ◽

Reverse Transcription ◽

Low Cost ◽

Pcr Assay ◽

Reverse Transcription Pcr ◽

Virus Genotypes ◽

Hepatitis C Virus Genotypes ◽

Development And Validation

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Real-time quantitative reverse transcription-PCR assay for renal cell carcinoma-associated antigen G250

Clinica Chimica Acta ◽

10.1016/s0009-8981(01)00799-9 ◽

2002 ◽

Vol 318 (1-2) ◽

pp. 33-40 ◽

Cited By ~ 9

Author(s):

Ye Chuanzhong ◽

Guan Ming ◽

Zhang Fanglin ◽

Chen Haijiao ◽

Lin Zhen ◽

...

Keyword(s):

Renal Cell Carcinoma ◽

Cell Carcinoma ◽

Real Time ◽

Reverse Transcription ◽

Renal Cell ◽

Pcr Assay ◽

Quantitative Reverse Transcription Pcr ◽

Reverse Transcription Pcr

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Rapid and Quantitative Detection of Hepatitis A Virus from Green Onion and Strawberry Rinses by Use of Real-Time Reverse Transcription-PCR

Applied and Environmental Microbiology ◽

10.1128/aem.71.9.5624-5626.2005 ◽

2005 ◽

Vol 71 (9) ◽

pp. 5624-5626 ◽

Cited By ~ 34

Author(s):

X. C. Shan ◽

P. Wolffs ◽

M. W. Griffiths

Keyword(s):

Real Time ◽

Reverse Transcription ◽

Hepatitis A ◽

Hepatitis A Virus ◽

Quantitative Detection ◽

Pcr Assay ◽

Reverse Transcription Pcr ◽

Immunomagnetic Capture ◽

Capture Method

ABSTRACT In this study, an immunomagnetic capture method and a real-time reverse transcription-PCR assay were used to quantify hepatitis A virus (HAV) in green onion and strawberry rinses. This combined protocol detected as low as 0.5 PFU HAV in produce rinses and concentrated HAV levels up to 20-fold.

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