Rapid Detection of Mycosphaerella graminicola in Wheat Using Reverse Transcription-PCR Assay

2005 ◽  
Vol 153 (11-12) ◽  
pp. 674-679 ◽  
Author(s):  
J.-R. Guo ◽  
F. Schnieder ◽  
M. Beyer ◽  
J.-A. Verreet
Keyword(s):  
Reverse Transcription ◽  
Rapid Detection ◽  
Mycosphaerella Graminicola ◽  
Pcr Assay ◽  
Reverse Transcription Pcr

scholarly journals Implementation of an in-house real-time reverse transcription-PCR assay for the rapid detection of the SARS-CoV-2 Marseille-4 variant

2021 ◽  
pp. 104814
Author(s):  
Marielle Bedotto ◽  
Pierre-Edouard Fournier ◽  
Linda Houhamdi ◽  
Anthony Levasseur ◽  
Jeremy Delerce ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Reverse Transcription Pcr

scholarly journals Reverse transcription-PCR assay for the rapid detection of viable Vibrio cholerae from fresh and processed shrimp

2017 ◽  
Vol 64 ◽  
Author(s):  
G. Jeyasekaran ◽  
M. Palanikumar ◽  
A. Varatharajakumar ◽  
R. Jeya Shakila
Keyword(s):  
Vibrio Cholerae ◽  
Reverse Transcription ◽  
Rapid Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Product Size ◽  
Reverse Transcription Pcr ◽  
Food Borne ◽  
Pcr Product ◽  
Shrimp Tissue

Viable Vibrio cholerae in seafood was rapidly detected by the Reverse transcription-PCR (RT-PCR) assay developed by targeting mRNA ctxA gene without any pre-enrichment. The PCR product size of the selected gene was 308 bp, which aided in the identification of V. cholerae. This RT-PCR assay was rapid, as it detected V. cholerae from fresh shrimp tissue within 5 min on bio-inoculation when the cell count was 1,000,000 cells. The pathogen was detected in 5 h when the load was 110 cells. The assay even detected the pathogen in shrimp that has been cooked for 10 min, frozen for 30 days and even in dried shrimp. Other food-borne pathogens like Salmonella, Staphylococcus aureus and Listeria monocytogenes were not detected by this assay. The developed RT-PCR assay is thus specific and rapid in detecting the viable Vibrio cholerae in seafood without any pre-enrichment.

scholarly journals Implementation of an in-house real-time reverse transcription-PCR assay to detect the emerging SARS-CoV-2 N501Y variants

2021 ◽  
pp. 104868
Author(s):  
Marielle BEDOTTO ◽  
Pierre-Edouard FOURNIER ◽  
Linda HOUHAMDI ◽  
Philippe COLSON ◽  
Didier RAOULT
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Pcr Assay ◽  
Reverse Transcription Pcr

Comet and cup genes in Drosophila spermatogenesis: the first demonstration of post-meiotic transcription

2008 ◽  
Vol 36 (3) ◽  
pp. 540-542 ◽  
Author(s):  
Carine Barreau ◽  
Elizabeth Benson ◽  
Helen White-Cooper
Keyword(s):  
In Situ Hybridization ◽  
Expression Pattern ◽  
Reverse Transcription ◽  
Protein Product ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Single Cyst ◽  
Reverse Transcription Pcr

Post-meiotic transcription is widespread in mammalian spermatogenesis, but is generally believed to be absent from Drosophila spermatogenesis. Genes required during meiosis, in early spermatids or later in spermiogenesis are typically transcribed in primary spermatocytes in Drosophila. Their mRNAs are then stored in the cytoplasm until the protein product is needed. Recently, using in situ hybridization, we identified 17 Drosophila genes, collectively named ‘comets’ and ‘cups’, whose mRNAs are most abundant in, and localize to the distal ends of, elongating spermatids. Using a single-cyst quantitative RT–PCR (reverse transcription–PCR) assay, we confirmed this unusual expression pattern and conclusively demonstrate the existence of post-meiotic transcription in Drosophila spermatids. We found that transcription of comets and cups occurs just before protamines can be detected in spermatid nuclei.

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