Integrated system of multiple batches to evaluate the continuous performance of microbial cells in decolourization processes

The IRμs™ is the first fully integrated system for Fourier transform infrared (FT-IR) microscopy. FT-IR microscopy combines light microscopy for morphological examination with infrared spectroscopy for chemical identification of microscopic samples or domains. Because the IRμs system is a new tool for molecular microanalysis, its optical, mechanical and system design are described to illustrate the state of development of molecular microanalysis. Applications of infrared microspectroscopy are reviewed by Messerschmidt and Harthcock.Infrared spectral analysis of microscopic samples is not a new idea, it dates back to 1949, with the first commercial instrument being offered by Perkin-Elmer Co. Inc. in 1953. These early efforts showed promise but failed the test of practically. It was not until the advances in computer science were applied did infrared microspectroscopy emerge as a useful technique. Microscopes designed as accessories for Fourier transform infrared spectrometers have been commercially available since 1983. These accessory microscopes provide the best means for analytical spectroscopists to analyze microscopic samples, while not interfering with the FT-IR spectrometer’s normal functions.

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Fate of sperm membrane in fertilized ova

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100146047 ◽

1993 ◽

Vol 51 ◽

pp. 40-41

Author(s):

Frank J. Longo

Keyword(s):

Electron Microscopy ◽

Electrical Activity ◽

Sea Urchins ◽

Morphological Changes ◽

Integrated System ◽

Electrophysiological Recording ◽

Experimental Conditions ◽

The Status ◽

Sperm Membrane ◽

Temporal And Spatial

Measurement of the egg's electrical activity, the fertilization potential or the activation current (in voltage clamped eggs), provides a means of detecting the earliest perceivable response of the egg to the fertilizing sperm. By using the electrical physiological record as a “real time” indicator of the instant of electrical continuity between the gametes, eggs can be inseminated with sperm at lower, more physiological densities, thereby assuring that only one sperm interacts with the egg. Integrating techniques of intracellular electrophysiological recording, video-imaging, and electron microscopy, we are able to identify the fertilizing sperm precisely and correlate the status of gamete organelles with the first indication (fertilization potential/activation current) of the egg's response to the attached sperm. Hence, this integrated system provides improved temporal and spatial resolution of morphological changes at the site of gamete interaction, under a variety of experimental conditions. Using these integrated techniques, we have investigated when sperm-egg plasma membrane fusion occurs in sea urchins with respect to the onset of the egg's change in electrical activity.

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Simultaneous ERP and PET studies of continuous performance tasks

Electroencephalography and Clinical Neurophysiology ◽

10.1016/s0013-4694(97)87983-0 ◽

1997 ◽

Vol 103 (1) ◽

pp. 18

Author(s):

K Nagata

Keyword(s):

Continuous Performance ◽

Performance Tasks ◽

Continuous Performance Tasks

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Contingent Negative Variation (CNV) bei Kindern mit hyperkinetischem Syndrom - eine experimentelle Untersuchung mittels des Continuous Performance Test (CPT)

Zeitschrift für Kinder- und Jugendpsychiatrie und Psychotherapie ◽

10.1024//1422-4917.28.4.239 ◽

2000 ◽

Vol 28 (4) ◽

pp. 239-246 ◽

Cited By ~ 25

Author(s):

K. Hennighausen ◽

G. Schulte-Körne ◽

A. Warnke ◽

H. Remschmidt

Keyword(s):

Contingent Negative Variation ◽

Performance Test ◽

Continuous Performance Test ◽

Continuous Performance ◽

Experimentelle Untersuchung

Zusammenfassung Fragestellung: Gibt es neurophysiologische Korrelate der Aufmerksamkeitsstörung beim hyperkinetischen Syndrom (HKS) und welche Bedeutung haben diese für die Ätiologie der Störung. Methodik: Selektive Aufmerksamkeitsprozesse wurden anhand des zweistufigen Continuous Performance Test (CPT) bei 18 Jungen mit hyperkinetischem Syndrom (HKS) untersucht und mit einer nach dem Alter parallelisierten Kontrollgruppe von 21 Jungen verglichen. Die Altersspanne der Stichprobe betrug 6 bis 12 Jahre. Parallel dazu wurden ereigniskorrelierte Potentiale (EKP) während des Tests an den Elektrodenpositionen Fz, Cz, Pz und Oz mit Referenz zu verbundenen Ohren abgeleitet. Ergebnisse: Im EKP nach dem präparatorischen Stimulus konnten zwei Komponenten der Contingent Negative Variation (CNV) mit unterschiedlicher topographischer Verteilung identifiziert werden (CNV-1: 600 bis 1100 ms und CNV-2: 1000 bis 1500 ms nach Stimulus). Die Stichproben unterschieden sich nicht auf der Verhaltensebene (Fehlerrate und Reaktionszeit). Signifikante Gruppenunterschiede ergaben sich hinsichtlich der Topographie der beiden CNV-Komponenten. Kinder mit HKS zeigten im Vergleich zu Kontrollkindern eine signifikant niedrigere CNV-1 über der frontalen und eine Tendenz zu stärkerer Negativierung (CNV-1 und CNV-2) über der occipitalen Elektrode. Schlussfolgerungen: Die Ergebnisse unterstützen die Hypothese einer Unterfunktion frontaler inhibitorischer Prozesse bei Kindern mit HKS.

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Comparing the Mathematics Continuous Performance Test With Other Measures of Tests of Attention

European Journal of Psychological Assessment ◽

10.1027/1015-5759/a000261 ◽

2016 ◽

Vol 32 (4) ◽

pp. 291-297 ◽

Cited By ~ 1

Author(s):

Dubi Lufi ◽

Shachar Pan

Keyword(s):

Performance Test ◽

Continuous Performance Test ◽

Clinical Population ◽

Control Group ◽

Continuous Performance ◽

Adhd Assessment ◽

Research Reporting ◽

Hyperactivity Disorder ◽

Continuous Performance Tests ◽

Better Than

Abstract. Several studies have shown that Continuous Performance Tests (CPT) can diagnose Attention Deficit Hyperactivity Disorder (ADHD) better than other tests. Research reporting comparisons of two or more CPT-type tests is scarce. The purpose of the study was to compare the Mathematics Continuous Performance Test (MATH-CPT) with another CPT-type test (CPT II) and a questionnaire (the Brown Scale). The comparison was carried out by looking at correlations among subscales and checking the precision of detecting ADHD. Ninety-five high school and college students participated in the study, 41 with ADHD were the research group and 54 were the control group. The participants performed the two tests and answered the questionnaire. The results showed that the MATH-CPT correctly identified 74.50% of the participants of both groups as compared to the 71.60% of the CPT II. Correlations between the two CPT-type tests were moderate; however, they were similar to correlations found in other studies comparing similar tools. The MATH-CPT, final attention formula, showed significant correlations with the Brown scales, while the CPT II, confidence index associated with ADHD assessment, showed nonsignificant correlations with the questionnaire. The study indicated that MATH-CPT can be used with a clinical population of ADHD and for research purposes.

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