FR-IR Molecular Microanalysis System

1990 ◽  
Vol 48 (2) ◽  
pp. 270-271
Author(s):  
John A. Reffner ◽  
William T. Wihlborg
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Integrated System ◽  
Morphological Examination ◽  
Fully Integrated ◽  
Ir Microscopy ◽  
Normal Functions ◽  
Infrared Microspectroscopy ◽  
Infrared Spectral ◽  
Ft Ir

The IRμs™ is the first fully integrated system for Fourier transform infrared (FT-IR) microscopy. FT-IR microscopy combines light microscopy for morphological examination with infrared spectroscopy for chemical identification of microscopic samples or domains. Because the IRμs system is a new tool for molecular microanalysis, its optical, mechanical and system design are described to illustrate the state of development of molecular microanalysis. Applications of infrared microspectroscopy are reviewed by Messerschmidt and Harthcock.Infrared spectral analysis of microscopic samples is not a new idea, it dates back to 1949, with the first commercial instrument being offered by Perkin-Elmer Co. Inc. in 1953. These early efforts showed promise but failed the test of practically. It was not until the advances in computer science were applied did infrared microspectroscopy emerge as a useful technique. Microscopes designed as accessories for Fourier transform infrared spectrometers have been commercially available since 1983. These accessory microscopes provide the best means for analytical spectroscopists to analyze microscopic samples, while not interfering with the FT-IR spectrometer’s normal functions.

scholarly journals Advanced Drug-Eluting Poly (Vinyl Chloride) Surfaces Deposited by Spin Coating

Medicina ◽  
2019 ◽  
Vol 55 (8) ◽  
pp. 421
Author(s):  
Oana Cristina Duta ◽  
Maxim Maximov ◽  
Roxana Trusca ◽  
Anton Ficai ◽  
Denisa Ficai ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Side Effects ◽  
Spin Coating ◽  
Large Scale ◽  
Fourier Transform Infrared ◽  
Culture Collection ◽  
Ir Microscopy ◽  
Good Ability ◽  
Deposition Parameters ◽  
Ft Ir

Background and objectives: Medical devices such as catheters are used on a large scale to treat heart and cardiovascular diseases. Unfortunately, they present some important drawbacks (structure failure, calcifications, infections, thrombosis, etc.), with the main side effects occurring due to adhesion and proliferation of bacteria and living cells on the surface of the implanted devices. The aim of this work is to modify the surface of polyvinyl chloride (PVC), an affordable biocompatible material, in order to reduce these aforementioned side effects. Materials and Methods: The surface of PVC was modified by depositing a thin layer also of PVC that incorporates an active substance, dicoumarol (a well-known anticoagulant), by spin coating process. The modified surfaces were analyzed by Fourier-transform infrared (FT-IR) microscopy, Fourier-transform infrared (FT-IR) spectroscopy, Ultraviolet-visible spectroscopy (UV-VIS), and Scanning electron microscopy (SEM) in order to determine the surface morphology and behavior. The samples were tested for Gram-positive (S. aureus ATCC 25923) and Gram-negative (P. aeruginosa ATCC 27853) standard strains from American Type Culture Collection (ATCC). Results: The material obtained had a smooth surface with a uniform distribution of dicoumarol, which is released depending on the deposition parameters. The concentration of dicoumarol at the surface of the material and also the release rate is important for the applications for which the surface modification was designed. PVC modified using the proposed method showed a good ability to prevent salt deposition and decreased the protein adhesion, and the resistance to bacterial adherence was improved compared with standard PVC.

Forensic Analysis of Architectural Finishes Using Fourier Transform Infrared and Raman Spectroscopy, Part II: White Paint

2005 ◽  
Vol 59 (11) ◽  
pp. 1340-1346 ◽  
Author(s):  
Steven E. J. Bell ◽  
Louise A. Fido ◽  
S. James Speers ◽  
W. James Armstrong ◽  
Sharon Spratt
Keyword(s):  
Raman Spectroscopy ◽  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Forensic Analysis ◽  
The Other ◽  
Large Group ◽  
Ir Microscopy ◽  
Infrared And Raman Spectroscopy ◽  
Ft Ir ◽  
Ir And Raman

White household paints are commonly encountered as evidence in the forensic laboratory but they often cannot be readily distinguished by color alone so Fourier transform infrared (FT-IR) microscopy is used since it can sometimes discriminate between paints prepared with different organic resins. Here we report the first comparative study of FT-IR and Raman spectroscopy for forensic analysis of white paint. Both techniques allowed the 51 white paint samples in the study to be classified by inspection as either belonging to distinct groups or as unique samples. FT-IR gave five groups and four unique samples; Raman gave seven groups and six unique samples. The basis for this discrimination was the type of resin and/or inorganic pigments/extenders present. Although this allowed approximately half of the white paints to be distinguished by inspection, the other half were all based on a similar resin and did not contain the distinctive modifiers/pigments and extenders that allowed the other samples to be identified. The experimental uncertainty in the relative band intensities measured using FT-IR was similar to the variation within this large group, so no further discrimination was possible. However, the variation in the Raman spectra was larger than the uncertainty, which allowed the large group to be divided into three subgroups and four distinct spectra, based on relative band intensities. The combination of increased discrimination and higher sample throughput means that the Raman method is superior to FT-IR for samples of this type.

scholarly journals Fourier-Transform Infrared Microspectroscopy (FT-IR) Study on Caput and Cauda Mouse Spermatozoa

Proceedings ◽  
2019 ◽  
Vol 42 (1) ◽  
pp. 19 ◽  
Author(s):  
Marianna Portaccio ◽  
Sonia Errico ◽  
Teresa Chioccarelli ◽  
Gilda Cobellis ◽  
Maria Lepore
Keyword(s):  
Fourier Transform ◽  
Fourier Transform Infrared ◽  
Biochemical Changes ◽  
Infrared Microspectroscopy ◽  
Fourier Transform Infrared Microspectroscopy ◽  
In Vitro Investigation ◽  
Ft Ir ◽  
Protein Nucleic Acid ◽  
Ir Study

Fourier-Transform Infrared micro-spectroscopy (µFT-IR) was used for an in vitro investigation on spermatozoa (SPZ) samples separately collected from caput and cauda of mouse epididymis. SPZ are characterized by deep biochemical changes during the transit along the epididymis and they can constitute ideal candidates for a µFT-IR investigation, thanks to the ability of this technique in analyzing cells at a molecular level. Appreciable differences were reported in the infrared spectra from caput and cauda SPZ, and biochemical changes in protein, nucleic acid, lipid, and carbohydrate content of cells were evidenced. The present investigation indicates that µFT-IR can constitute a valuable tool for monitoring, in an easy and fast way, the changes suffered by SPZ during the transit along the epididymis.

Characterising cytotoxic agent action as a function of the cell cycle using fourier transform infrared microspectroscopy

The Analyst ◽  
2015 ◽  
Vol 140 (13) ◽  
pp. 4453-4464 ◽  
Author(s):  
M. Jimenez-Hernandez ◽  
M. D. Brown ◽  
C. Hughes ◽  
N. W. Clarke ◽  
P. Gardner
Keyword(s):  
Cell Cycle ◽  
Fourier Transform ◽  
Cellular Response ◽  
Fourier Transform Infrared ◽  
Cell Interaction ◽  
Cytotoxic Agent ◽  
Spectral Signatures ◽  
Infrared Microspectroscopy ◽  
Fourier Transform Infrared Microspectroscopy ◽  
Infrared Spectral

Infrared spectral signatures of drug–cell interaction, suggest that both the stages of proliferation and the degree of apoptosis need to be taken into account to elucidate the fine biochemical details of the immediate cellular response to the drug.

Simultaneous Orthogonal Drug Detection Using Fully Integrated Gas Chromatography with Fourier Transform Infrared Detection and Mass Spectrometric Detection

2016 ◽  
Vol 71 (5) ◽  
pp. 1050-1059 ◽  
Author(s):  
Adam Lanzarotta ◽  
Travis Falconer ◽  
Heather McCauley ◽  
Lisa Lorenz ◽  
Douglas Albright ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Fourier Transform ◽  
Synthetic Cannabinoids ◽  
Fourier Transform Infrared ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Infrared Detection ◽  
Ir Detector ◽  
Fully Integrated ◽  
Ft Ir

Analytes that co-elute and yield nearly identical electron ionization (EI) mass spectra, as well as analytes that yield non-specific EI fragmentation patterns, have been identified using fully integrated gas chromatography with direct deposit Fourier transform infrared detection and mass spectrometric detection (GC/FT-IR/MS). While the IR detector proved to be more selective for identifying analytes such as synthetic cannabinoids and weight loss drugs, it was limited by a relatively high detection limit of 8.4 parts per million (ppm) for non-targeted identification of sibutramine based on a single injection but was reduced to 840  parts per billion (ppb) for targeted identification of sibutramine by redepositing ten injections along the same track. The MS detector was less selective for identifying these analytes but yielded non-targeted and targeted detection limits of approximately 84 ppb and 8.4 ppb, respectively, which corresponded to a 100-fold advantage compared to the IR detector. Overall, the results of this study demonstrate that the advantages of each detector compensate for the limitations of the other, which allows a wider range of analytes and concentrations to be examined using a fully integrated GC/FT-IR/MS instrument compared to what can be examined using GC/IR or GC/MS independently. Not only does this approach reduce consumption of laboratory resources and time, it provides IR and MS information on the same sample, which is important for forensic analyses that require data from two or more orthogonal techniques to make an identification.

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