Co-digestion of garden waste, food waste, and tofu residue: Effects of mixing ratio on methane production and microbial community structure

Author(s):  
Yingjin Song ◽  
Shuyan Meng ◽  
Guanyi Chen ◽  
Beibei Yan ◽  
Yingxiu Zhang ◽  
...  
2020 ◽  
Vol 96 (2) ◽  
Author(s):  
Kärt Kanger ◽  
Nigel G H Guilford ◽  
HyunWoo Lee ◽  
Camilla L Nesbø ◽  
Jaak Truu ◽  
...  

ABSTRACT Solid organic waste is a significant source of antibiotic resistance genes (ARGs) and effective treatment strategies are urgently required to limit the spread of antimicrobial resistance. Here, we studied ARG diversity and abundance as well as the relationship between antibiotic resistome and microbial community structure within a lab-scale solid-state anaerobic digester treating a mixture of food waste, paper and cardboard. A total of 10 samples from digester feed and digestion products were collected for microbial community analysis including small subunit rRNA gene sequencing, total community metagenome sequencing and high-throughput quantitative PCR. We observed a significant shift in microbial community composition and a reduction in ARG diversity and abundance after 6 weeks of digestion. ARGs were identified in all samples with multidrug resistance being the most abundant ARG type. Thirty-two per cent of ARGs detected in digester feed were located on plasmids indicating potential for horizontal gene transfer. Using metagenomic assembly and binning, we detected potential bacterial hosts of ARGs in digester feed, which included Erwinia, Bifidobacteriaceae, Lactococcus lactis and Lactobacillus. Our results indicate that the process of sequential solid-state anaerobic digestion of food waste, paper and cardboard tested herein provides a significant reduction in the relative abundance of ARGs per 16S rRNA gene.


2020 ◽  
Vol 317 ◽  
pp. 123948
Author(s):  
Yanzeng Li ◽  
Zhou Chen ◽  
Yanyan Peng ◽  
Kaiming Zheng ◽  
Chengsong Ye ◽  
...  

Fuel ◽  
2018 ◽  
Vol 211 ◽  
pp. 600-608 ◽  
Author(s):  
Katherine J. Davis ◽  
Shipeng Lu ◽  
Elliott P. Barnhart ◽  
Albert E. Parker ◽  
Matthew W. Fields ◽  
...  

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