Chlorella vulgaris cultivation in sludge extracts from 2,4,6-TCP wastewater treatment for toxicity removal and utilization

2017 ◽  
Vol 187 ◽  
pp. 146-153 ◽  
Author(s):  
Lu Wang ◽  
Xiurong Chen ◽  
Hualin Wang ◽  
Yuying Zhang ◽  
Qingjie Tang ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Chlorella Vulgaris ◽  
Sludge Extracts

Biosorptio Of Cd2+ By Wildtype And Cadmium Resistant, CdR-99 Cell Line Of Chlorella Vulgaris

2021 ◽  
Vol 14 (14) ◽  
pp. 115-123
Author(s):  
Sudarshana Shakya ◽  
Bindra Devi Shakya ◽  
Amrita Tamrakar ◽  
Bijaya Adhikari ◽  
Sabita Nepal ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Wastewater Treatment ◽  
Cell Line ◽  
Cell Lines ◽  
Chlorella Vulgaris ◽  
Algal Cell ◽  
Resistant Isolate ◽  
Resistant Line ◽  
Wastewater Treatment Process ◽  
Flame Atomic Absorption

Removal of heavy metals is very important in wastewater treatment process, due to their toxic effects on the environment. Biological treatment has attracted researchers for years since it has many advantages over physical and chemical methods for removing heavy metals from wastewater. The purpose of this research was to assess the biosorption of Cd2+ by wildtype (WT) and CdR-99 resistant line of Chlorella vulgaris confirming mechanisms of resistance to Cd2+ toxicity and the effect of the variable concentrations of Cd2+ on their growth. Exposure of both algal cell lines to increasing Cd2+ concentrations resulted in progressive inhibition of growth as revealed by growth experiments. The higher ID50 value (38 μM Cd2+) of CdR-99 resistant line exhibited some degree of resistance to Cd2+ toxicity. Metal content was determined by flame atomic absorption spectrometry (FAAS). When exposed to the growth medium containing 50 μM Cd2+, CdR-99 resistant isolate proved to be efficient cell line compared to the WT, in terms of adsorption and removal of Cd2+ at 15 min and 48 hr interval of time respectively. Extracellular Cd2+ adsorption was found significantly higher than intracellular uptake in both the tested cell lines. Total Cd2+ accumulation and distribution between the external and internal cell fractions of the CdR-99 were significantly higher to the WT. Thus, the CdR-99 cell line appeared more resistant to Cd2+ toxicity and hence may be used for wastewater treatment and remediation of metal contaminated sites.

scholarly journals Municipal Wastewater: A Sustainable Source for the Green Microalgae Chlorella vulgaris Biomass Production

2021 ◽  
Vol 11 (5) ◽  
pp. 2207
Author(s):  
Diana Pacheco ◽  
A. Cristina S. Rocha ◽  
Analie Garcia ◽  
Ana Bóia ◽  
Leonel Pereira ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Biomass Production ◽  
Chlorella Vulgaris ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Basal Medium ◽  
Chlorophyll Synthesis ◽  
Secondary Effluent ◽  
Municipal Wastewater Treatment ◽  
Run Off

The need to reduce the costs associated with microalgae cultivation encouraged scientific research into coupling this process with wastewater treatment. Thus, the aim of this work was to assess the growth of Chlorella vulgaris (Chlorophyta) in different effluents from a municipal wastewater treatment plant (WWTP), namely secondary effluent (SE) and sludge run-off (SR). Assays were performed, under the same conditions, in triplicate with 4 dilution ratios of the wastewaters (25%, 50%, 75% and 100%) with the standard culture medium bold basal medium double nitrated (BBM2N) as a control. The capability of C. vulgaris for biomass production, chlorophyll synthesis and nutrients removal in the SE and SR was evaluated. The 25% SE and 25% SR showed increased specific growth rates (0.47 and 0.55 day−1, respectively) and higher biomass yields (8.64 × 107 and 1.95 × 107 cells/mL, respectively). Regarding the chlorophyll content, the 100% SR promoted the highest concentration of this pigment (2378 µg/L). This green microalga was also able to remove 94.8% of total phosphorus of SE, while in 50% SR, 31.2% was removed. Removal of 73.9% and 65.9% of total nitrogen in 50% and 100% SR, respectively, was also observed. C. vulgaris growth can, therefore, be maximized with the addition of municipal effluents, to optimize biomass production, while cleansing the effluents.

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