scholarly journals 714 Study of the cytotoxic effect of cleanser on human skin keratinocytes culture in vitro and correlation in vivo

2018 ◽  
Vol 138 (5) ◽  
pp. S121
Author(s):  
M. Cornejo-Guerrero ◽  
D. Hernández-Blanco ◽  
D. Cortés-García ◽  
J. Castanedo-Cázares
Keyword(s):  
Human Skin ◽  
Cytotoxic Effect ◽  
Culture In Vitro ◽  
Skin Keratinocytes

scholarly journals Additional evidence for a proform to tropoelastin from chick aorta

1979 ◽  
Vol 177 (2) ◽  
pp. 559-567 ◽  
Author(s):  
C S Heng-Khoo ◽  
R B Rucker ◽  
K W Buckingham
Keyword(s):  
Basic Protein ◽  
Cross Linking ◽  
Protein Subunit ◽  
Deficient Diet ◽  
Culture In Vitro ◽  
One Step

Evidence is presented for the presence of precursor to tropoelastin in chick arterial extracts. The precursor is approx. 100 000 daltons in size. It is suggested to be a precursor to tropoelastin (72 000 daltons). This protein may be observed in culture in vitro if appropriate precautions are taken to inhibit proteolysis. Once synthesized, it appears to be converted into tropoelastin within 10–20 min. The protein may also be detected in vivo. When 1-day-old cockerels were fed on a copper-deficient diet (less than 1 p.p.m. to inhibit cross-linking) containing epsilon-aminohexanoic acid (0.2%) to retard proteolysis and then injected wiht [3H]valine, extraction of arterial proteins 12h after injection resulted in detection of two major peaks of [3H]valine-labelled protein with pI values of pH 7.0 and 5.0 respectively. The protein that focused at pH 7.0 was estimated to be about 100 000 daltons in size and could be shown to be converted into a more basic protein with the properties of tropoelastin. It is speculated that the protein with pI 5.0 may be yet another extension peptide. The data appear to be in keeping with similar observations by ourselves and others that a proform of tropoelastin exists, and, in at least one step before conversion into tropoelastin, exists as a 100 000-dalton protein subunit.

scholarly journals Effects of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans on phagocytic response of Eisenia andrei coelomocytes

2011 ◽  
Vol 1 (1) ◽  
pp. 6 ◽  
Author(s):  
Hayet Belmeskine ◽  
Pauline Brousseau ◽  
Sami Haddad ◽  
Louise Vandelac ◽  
Michel Fournier
Keyword(s):  
Flow Cytometry ◽  
Phagocytic Activity ◽  
Cytotoxic Effect ◽  
Polychlorinated Dibenzofurans ◽  
Eisenia Andrei ◽  
Phagocytic Capacity ◽  
Excessive Secretion ◽  
Phagocytic Response

The immunotoxicological effects of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs) mixtures on <em>Eisenia andrei </em>earthworms have never been studied. In this work we investigated these effects both for <em>in vitro </em>and <em>in vivo</em> exposure, using the viability and the phagocytic activity of coelomocytes as immunological biomarkers and the flow cytometry was used for analysis. The <em>in vitro </em>exposure revealed a cytotoxic effect of PCDD/Fs mixture (C2) containing 50&yen;10-3 ng/mL of 2, 3, 7, 8-TCDD and an induction of the phagocytic capacity at the mixture (C1) containing 25&yen;10-3 ng/mL of 2, 3, 7, 8-TCDD. In the <em>in vivo </em>filter paper exposure, the immunocompetence of earthworms was assessed after 3 h-exposure to mixtures of PCDD/Fs at the levels of C1, C2, C3 and C4 containing about; 0.05, 0.3, 0.5 and 0.83 ng of 2, 3, 7, 8-TCDD/cm&sup2;, respectively. Morphological observations showed an excessive secretion of mucus and body surface lesions in worms exposed to higher concentrations (C3 and C4), which revealed that these organisms were affected by PCDD/Fs either through skin and/or by feeding. The levels of the extruded cell yield decreased significantly at all the concentrations tested. However, the cell viability was shown to be unaffected by PCDD/Fs concentrations. It was also shown, that exposure to the highest PCDD/Fs concentrations; C2, C3 and C4 inhibited both phagocytic activity and efficiency.

Human Skin Binding and Absorption of Contaminants from Ground and Surface Water During Swimming and Bathing

1989 ◽  
Vol 8 (5) ◽  
pp. 853-859 ◽  
Author(s):  
Ronald C. Wester ◽  
Howard I. Maibach
Keyword(s):  
Surface Water ◽  
Stratum Corneum ◽  
Human Skin ◽  
The Body ◽  
Water Soluble ◽  
Exposure Effect ◽  
Human Stratum Corneum ◽  
Water Dilution

Contaminants exist in ground and surface water. Human skin has the capacity to bind and then absorb these contaminants into the body during swimming and bathing. Powdered human stratum corneum will bind both lipid-soluble (alachlor, polychlorinated biphenyls [PCBs], benzene) and water-soluble (nitroaniline) chemicals. In vitro (human skin) and in vivo (Rhesus monkey) studies show that these chemicals readily distribute into skin, and then some of the chemical is absorbed into the body. Linearity in binding and absorption exists for nitroaniline over a 10-fold concentration range. Multiple exposure to benzene is at least cumulative. Binding and absorption can be significant for exposures as short as 30 min, and will increase with time. Absorption with water dilution increased for alachlor, but not for dinoseb. Soap reversed the partitioning of alachlor between human stratum corneum and water. The PCBs could be removed from skin by soap and water (70% efficiency) for up to 3 h and then decontamination potential decreased, due to continuing skin absorption. The model in vitro and in vivo systems used should permit easy estimation of this area of extensive human exposure effect on risk assessment.

Topical stabilized retinol treatment induces the expression of HAS genes and HA production in human skin in vitro and in vivo

2017 ◽  
Vol 309 (4) ◽  
pp. 275-283 ◽  
Author(s):  
Wen-Hwa Li ◽  
Heng-Kuan Wong ◽  
José Serrano ◽  
Manpreet Randhawa ◽  
Simarna Kaur ◽  
...  
Keyword(s):  
Human Skin
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