Inflorescence bulbils of tiger lily in vivo and bulbils culture in vitro

Evidence is presented for the presence of precursor to tropoelastin in chick arterial extracts. The precursor is approx. 100 000 daltons in size. It is suggested to be a precursor to tropoelastin (72 000 daltons). This protein may be observed in culture in vitro if appropriate precautions are taken to inhibit proteolysis. Once synthesized, it appears to be converted into tropoelastin within 10–20 min. The protein may also be detected in vivo. When 1-day-old cockerels were fed on a copper-deficient diet (less than 1 p.p.m. to inhibit cross-linking) containing epsilon-aminohexanoic acid (0.2%) to retard proteolysis and then injected wiht [3H]valine, extraction of arterial proteins 12h after injection resulted in detection of two major peaks of [3H]valine-labelled protein with pI values of pH 7.0 and 5.0 respectively. The protein that focused at pH 7.0 was estimated to be about 100 000 daltons in size and could be shown to be converted into a more basic protein with the properties of tropoelastin. It is speculated that the protein with pI 5.0 may be yet another extension peptide. The data appear to be in keeping with similar observations by ourselves and others that a proform of tropoelastin exists, and, in at least one step before conversion into tropoelastin, exists as a 100 000-dalton protein subunit.

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Mouse Periosteal Cell Culture, in vitro Differentiation, and in vivo Transplantation in Tibial Fractures

BIO-PROTOCOL ◽

10.21769/bioprotoc.4107 ◽

2021 ◽

Vol 11 (15) ◽

Author(s):

Simon Perrin ◽

Anais Julien ◽

Oriane Duchamp de Lageneste ◽

Rana Abou-Khalil ◽

Céline Colnot

Keyword(s):

Cell Culture ◽

Tibial Fractures ◽

In Vitro Differentiation ◽

Culture In Vitro ◽

Periosteal Cell

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Morphological and physiological features of the miniature rose cultivar ‘Rise’n’Shine’ under long time culture in vitro and in vivo

Acta Horticulturae ◽

10.17660/actahortic.2018.1224.19 ◽

2018 ◽

pp. 139-144

Author(s):

V.A. Brailko ◽

S.A. Plugatar ◽

T.I. Pilipchuk ◽

Yu.V. Plugatar ◽

I.V. Mitrofanova

Keyword(s):

Culture In Vitro ◽

Long Time ◽

Time Culture

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5 -Reductase Type 2 Is Constitutively Expressed in the Dermal Papilla and Connective Tissue Sheath of the Hair Follicle in Vivo But Not during Culture in Vitro

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jc.86.6.2875 ◽

2001 ◽

Vol 86 (6) ◽

pp. 2875-2880 ◽

Cited By ~ 18

Author(s):

Y. Asada

Keyword(s):

Connective Tissue ◽

Hair Follicle ◽

Dermal Papilla ◽

Culture In Vitro ◽

Connective Tissue Sheath

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Histology of larval eye—antennal disks and cephalic ganglia of Drosophila cultured in vitro

Development ◽

10.1242/dev.15.3.271 ◽

1966 ◽

Vol 15 (3) ◽

pp. 271-279

Author(s):

Imogene Schneider

Keyword(s):

Drosophila Melanogaster ◽

Larval Instar ◽

Prepupal Stage ◽

Culture In Vitro ◽

Histological Studies ◽

The Past

In the past decade, a relatively large number of reports have been published on the culture in vitro of organs of Drosophila melanogaster. The majority of these reports have been concerned with the development of the eye-antennal complex explanted, with or without the attached cephalic ganglia, from the prepupal stage (Demal, 1956) or from late second and third larval instar stages (Kuroda & Yamaguchi, 1956; Gottschewski, 1958, 1960, 1962; Gottschewski & Querner, 1961; Fugio, 1962; Schneider, 1964). With the exception of the work of Demal (1956), the above reports have been confined to descriptions of the developing explants solely in morphological terms and, as such, are not wholly adequate for comparisons to be made between development in vivo and in vitro. If, however, such descriptions are supplemented with histological studies, a more valid appreciation can be gained of the potentialities as well as the limitations of such explants under conditions in vitro.

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157 AGGREGATION AND DEVELOPMENT OF RABBIT CLONED EMBRYOS WITH ZYGOTIC, TETRAPLOID, AND PARTHENOGENETIC EMBRYO IN VITRO

Reproduction Fertility and Development ◽

10.1071/rdv22n1ab157 ◽

2010 ◽

Vol 22 (1) ◽

pp. 237

Author(s):

C.-H. Chen ◽

T.-A. Lin ◽

H.-Y. Su ◽

Y.-S. Sung ◽

L.-J. Sung ◽

...

Keyword(s):

Standard Procedure ◽

Cell Number ◽

Cell Counting ◽

Culture In Vitro ◽

Developmental Potential ◽

Counting Data ◽

Parthenogenetic Embryo ◽

Different Origin

The rabbit compared to other domestic animals, such as cattle and sheep, was a relatively more difficult species to clone. One of the major reasons may be attributed to the low cell number in cloned embryo before implantation. This study was designed to aggregate 2 nuclear transfer (NT) embryos and one with a different origin, determine their developmental potential in vitro, and finally examine the cell number of aggregated embryos. NT was performed with our standard procedure using in vivo derived oocytes and donor cells from adult skin fibroblasts. Zygotes (ZY) were collected from does at 18 h post-hCG and mating. Parthenogenetic (PA) embryos were generated from oocytes with activation protocol, whereas tetraploid embryos (4N) were prepared by fusing fertilized embryos at 2-celled stage into 1-celled stage by electrical pulse. All of the embryos were cultured for 20-24 h into 4-/8-celled stage prior to aggregation. Zona pellucida was then removed and 2 NT embryos were aggregated with 1 embryo originated from ZY, PA, and 4N groups in a depressed droplet containing culture medium. Aggregated embryos were cultured for another 48 h (total 3 days, initiation of activation = Day 0) before being fixed for cell counting. Both single embryo from NT, ZY, PA, and 4N and 3 embryo aggregates (3X) from the same category were used as controls for NT aggregation. The results of 3-day embryo culture in vitro showed that the development of aggregated embryos to blastocyst (BL) stage was 2 NT + ZY, 68.6% (n = 35); 2 NT + 4N, 91.7% (n = 36); and 2 NT+PA, 37.5% (n = 24), whereas 3X aggregates developed to BL at a rate of ZY, 100% (n = 24); 4N, 100% (n = 19); and PA, 100% (n = 14). The BL rates of single embryo control developed into early BL were ZY, 100% (n = 34) and 4N, 93% (n = 36); however, NT and PA developed slower, only 45.4% NT (n = 187) and 79.2% PA (n = 72) to compacted morula/early BL stage. Cell counting data (Table 1) showed that there was no difference in cell number per embryo between NT and PA, whereas ZY and 4N possessed significantly higher cell number than NT and PA (128-162 v. 52-61, P < 0.05) for single embryo category. In the 3X aggregation group, significantly higher cell number per aggregated embryo was found in ZY and 4N compared to that in PA (549-564 v. 196, P < 0.05). More importantly, there was significantly higher cell number found in 2 NT + 4N embryo than that in single 4N (293 v. 162, P < 0.05). This result demonstrated that 2 cloned embryos had propagated and incorporated into 1 tetraploid embryo during pre-implantational development. The next step is to study how NT embryos successfully interact within the aggregated embryo during further development and differentiation, in order to increase the birth rate of clones. Table 1.The cell number of aggragated rabbit embryos between NT and other embryos of different origin after 3 days of culture in vitro Supported by NIH 5R44HL091605-03.

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Nouvelles observations sur les facteurs qui contrôlent l'érythropoïèse in vitro

Development ◽

10.1242/dev.22.1.15 ◽

1969 ◽

Vol 22 (1) ◽

pp. 15-25

Author(s):

Par Germano Salvatorelli

Keyword(s):

Nous Avons ◽

Culture In Vitro ◽

Sont Comparables

Nous avons démontré précédemment que l'association de fragments de foie embryonnaire à des explants de moelle osseuse de poulet s'est montrée très favorable au maintien du processus de l'érythropoïèse médullaire. Dans ces conditions expérimentales en effet, même après 15 jours de culture la structure et la composition cellulaire des explants sont comparables à celles existant in vivo. En particulier, en ce qui concerne la série rouge tous les stades de la différenciation cellulaire depuis la cellule souche jusqu'à la cellule sanguine mûre sont présents dans nos explants et dans des proportions comparables à celles qu'on retrouve dans la moelle osseuse normale. Nous en avons conclu que le tissu hépatique joue un rôle indispensable dans la différenciation et la maturation des éléments de la série rouge en culture in vitro et qu'il doit agir d'une façon spécifique car la culture de la moelle osseuse, seule ou bien en association avec le mésonéphros, ne permet qu'une survie très limitée des différentes lignées sanguines.

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Induction de cartilage in vitro par l'extrait d'otocystes d'embryons de Poulet

Development ◽

10.1242/dev.8.1.33 ◽

1960 ◽

Vol 8 (1) ◽

pp. 33-38

Author(s):

par Jean A. A. Benoit

Keyword(s):

Culture In Vitro ◽

Nous Pensons

En 1937 De Beer, dans The Development of the Vertebrate Skull, posait le problème suivant: ‘Can extracts of auditory vesicles induce the formation of cartilage in vitro? in vivo?’ (p. 514). Nous pensons avoir trouvé une réponse à la première question: l'extrait d'otocystes induit in vitro la formation de cartilage à partir du mésenchyme otique, chez l'embryon de Poulet. Des recherches antérieures nous avaient montré que l'épithélium auditif est l'inducteur normal de la capsule otique cartilagineuse (Benoit, 1955). D'autre part, lorsque l'ébauche otique épithéliale est déplacée au niveau des futures régions méningées et dermiques, le mésenchyme reste indifférent (Benoit, 1956). Certains territoires du mésenchyme céphalique sont done incapables de répondre à l'induction otique. De même le mésenchyme somitique associé à un otocyste in vivo ou in vitro, ne se différencie jamais en cartilage (Benoit, 1960). Nous n'avons done pu éprouver que le mésenchyme otique, en culture in vitro, sur un milieu renfermant l'extrait d'otocystes.

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