Optimization of intracellular cytokine staining for the quantitation of antigen-specific CD4+ T cell responses in rhesus macaques

2004 ◽  
Vol 288 (1-2) ◽  
pp. 61-79 ◽  
Author(s):  
Marie-Claire Gauduin ◽  
Amitinder Kaur ◽  
Shabbir Ahmad ◽  
Tilahun Yilma ◽  
Jeffrey D. Lifson ◽  
...  
Keyword(s):  
T Cell ◽  
Rhesus Macaques ◽  
Intracellular Cytokine Staining ◽  
T Cell Responses ◽  
Cd4 T Cell ◽  
Intracellular Cytokine ◽  
Cell Responses

scholarly journals Optimization and qualification of an 8-color intracellular cytokine staining assay for quantifying T cell responses in rhesus macaques for pre-clinical vaccine studies

2012 ◽  
Vol 386 (1-2) ◽  
pp. 10-21 ◽  
Author(s):  
Mitzi M. Donaldson ◽  
Shing-Fen Kao ◽  
Leila Eslamizar ◽  
Connie Gee ◽  
Gerrit Koopman ◽  
...  
Keyword(s):  
T Cell ◽  
Rhesus Macaques ◽  
Intracellular Cytokine Staining ◽  
T Cell Responses ◽  
Intracellular Cytokine ◽  
Cell Responses

CpG oligonucleotides induce strong humoral but only weak CD4+ T cell responses to protein antigens in rhesus macaques in vivo

Vaccine ◽  
2005 ◽  
Vol 23 (25) ◽  
pp. 3310-3317 ◽  
Author(s):  
Gunther Hartmann ◽  
Anja Marschner ◽  
Pablo Renner Viveros ◽  
Christiane Stahl-Hennig ◽  
Martin Eisenblätter ◽  
...  
Keyword(s):  
T Cell ◽  
Rhesus Macaques ◽  
T Cell Responses ◽  
Cd4 T Cell ◽  
Protein Antigens ◽  
Cell Responses ◽  
Cpg Oligonucleotides

scholarly journals Codelivery of Envelope Protein in Alum with MVA Vaccine Induces CXCR3-Biased CXCR5+and CXCR5−CD4 T Cell Responses in Rhesus Macaques

2015 ◽  
Vol 195 (3) ◽  
pp. 994-1005 ◽  
Author(s):  
Smita S. Iyer ◽  
Sailaja Gangadhara ◽  
Blandine Victor ◽  
Rosy Gomez ◽  
Rahul Basu ◽  
...  
Keyword(s):  
T Cell ◽  
Envelope Protein ◽  
Rhesus Macaques ◽  
T Cell Responses ◽  
Cd4 T Cell ◽  
Cell Responses

scholarly journals Development of a whole blood intracellular cytokine staining assay for mapping CD4+ and CD8+ T-cell responses across the HIV-1 genome

2007 ◽  
Vol 144 (1-2) ◽  
pp. 115-121 ◽  
Author(s):  
Stephen Meddows-Taylor ◽  
Sharon Shalekoff ◽  
Louise Kuhn ◽  
Glenda E. Gray ◽  
Caroline T. Tiemessen
Keyword(s):  
T Cell ◽  
Whole Blood ◽  
Intracellular Cytokine Staining ◽  
T Cell Responses ◽  
Cd8 T Cell ◽  
Intracellular Cytokine ◽  
Cell Responses ◽  
Hiv 1

scholarly journals Broadly directed SARS-CoV-2-specific CD4+ T cell response includes frequently detected peptide specificities within the membrane and nucleoprotein in patients with acute and resolved COVID-19

2021 ◽  
Vol 17 (9) ◽  
pp. e1009842
Author(s):  
Janna Heide ◽  
Sophia Schulte ◽  
Matin Kohsar ◽  
Thomas Theo Brehm ◽  
Marissa Herrmann ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Response ◽  
Ex Vivo ◽  
Intracellular Cytokine Staining ◽  
T Cell Responses ◽  
T Cell Response ◽  
Cd4 T Cell ◽  
Cell Responses ◽  
Single Peptide

The aim of this study was to define the breadth and specificity of dominant SARS-CoV-2-specific T cell epitopes using a comprehensive set of 135 overlapping 15-mer peptides covering the SARS-CoV-2 envelope (E), membrane (M) and nucleoprotein (N) in a cohort of 34 individuals with acute (n = 10) and resolved (n = 24) COVID-19. Following short-term virus-specific in vitro cultivation, the single peptide-specific CD4+ T cell response of each patient was screened using enzyme linked immuno spot assay (ELISpot) and confirmed by single-peptide intracellular cytokine staining (ICS) for interferon-γ (IFN-γ) production. 97% (n = 33) of patients elicited one or more N, M or E-specific CD4+ T cell responses and each patient targeted on average 21.7 (range 0–79) peptide specificities. Overall, we identified 10 N, M or E-specific peptides that showed a response frequency of more than 36% and five of them showed high binding affinity to multiple HLA class II binders in subsequent in vitro HLA binding assays. Three peptides elicited CD4+ T cell responses in more than 55% of all patients, namely Mem_P30 (aa146-160), Mem_P36 (aa176-190), both located within the M protein, and Ncl_P18 (aa86-100) located within the N protein. These peptides were further defined in terms of length and HLA restriction. Based on this epitope and restriction data we developed a novel DRB*11 tetramer (Mem_aa145-164) and examined the ex vivo phenotype of SARS-CoV-2-specific CD4+ T cells in one patient. This detailed characterization of single T cell peptide responses demonstrates that SARS-CoV-2 infection universally primes a broad T cell response directed against multiple specificities located within the N, M and E structural protein.

scholarly journals ELISPOT and intracellular cytokine staining: Novel assays for quantifying T cell responses in the chicken

2008 ◽  
Vol 32 (11) ◽  
pp. 1398-1404 ◽  
Author(s):  
M.P. Ariaans ◽  
P.M. van de Haar ◽  
J.W. Lowenthal ◽  
W. van Eden ◽  
E.J. Hensen ◽  
...  
Keyword(s):  
T Cell ◽  
Intracellular Cytokine Staining ◽  
T Cell Responses ◽  
Intracellular Cytokine ◽  
Cell Responses

Author response for "Naturally processed HLA‐DR3‐restricted HHV‐6b peptides are recognized broadly with polyfunctional and cytotoxic CD4 T cell responses"

2019 ◽  
Author(s):  
Aniuska Becerra‐Artiles ◽  
John Cruz ◽  
John D. Leszyk ◽  
John Sidney ◽  
Alessandro Sette ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Responses ◽  
Cd4 T Cell ◽  
Author Response ◽  
Cell Responses ◽  
Hla Dr3
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