Development and evaluation of a rapid direct DNA extraction and real-time PCR assay for early detection of methicillin resistant Staphylococcus aureus (MRSA) in high-risk patients

2007 ◽  
Vol 55 (3) ◽  
pp. e63
Author(s):  
Lucy Elston ◽  
TatShing Yam ◽  
Peter Marsh
Keyword(s):  
Staphylococcus Aureus ◽  
High Risk ◽  
Early Detection ◽  
Real Time ◽  
Dna Extraction ◽  
Real Time Pcr ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Pcr Assay ◽  
Risk Patients ◽  
Direct Dna Extraction

scholarly journals New Real-Time PCR Assay Using Locked Nucleic Acid Probes To Assess Prevalence of ParC Mutations in Fluoroquinolone-Susceptible Streptococcus pneumoniae Isolates from France

2006 ◽  
Vol 50 (4) ◽  
pp. 1594-1598 ◽  
Author(s):  
Jean-Winoc Decousser ◽  
Imen Methlouthi ◽  
Patrick Pina ◽  
Anne Collignon ◽  
Pierre Allouch
Keyword(s):  
Streptococcus Pneumoniae ◽  
Nucleic Acid ◽  
High Risk ◽  
Real Time ◽  
Real Time Pcr ◽  
Locked Nucleic Acid ◽  
Pcr Assay ◽  
Nucleic Acid Probes ◽  
National Surveys ◽  
Risk Patients

ABSTRACT A real-time PCR assay with locked nucleic acid probes was developed to screen mutations at codons 79 and 83 of the Streptococcus pneumoniae parC gene. Only silent mutations were detected among 236 French invasive fluoroquinolone-susceptible strains. This test could be useful for some high-risk patients or in national surveys.

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